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Exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin increases the activation of aryl hydrocarbon receptor and is associated with the aggressiveness of osteosarcoma MG-63 osteoblast-like cells

  • Authors:
    • Shih-Chieh Yang
    • Chin-Hsien Wu
    • Yuan-Kun Tu
    • Shin-Yu Huang
    • Pai-Chien Chou
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedic Surgery, E-Da Hospital, I-Shou University, Kaohsiung 82445, Taiwan, R.O.C., Department of Thoracic Medicine, Chang Gung Medical Foundation, Chang Gung University, College of Medicine, Taoyuan 33305, Taiwan, R.O.C., Department of Thoracic Medicine, Saint Paul's Hospital, Taoyuan 33069, Taiwan, R.O.C.
    Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3849-3857
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    Published online on: July 6, 2018
       https://doi.org/10.3892/ol.2018.9098
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Abstract

The aryl hydrocarbon receptor (AhR) is a ligand-dependent transcription factor whose activity is modulated by xenobiotics and physiological ligands. Activation of the AhR by environmental xenobiotics may induce a conformational change in AhR and has been implicated in a variety of cellular processes, including inflammation and tumorigenesis. It is unknown whether the activation of AhR serves a role in modulating the progression of osteosarcoma. The osteosarcoma cell line MG-63, was treated with AhR ligand, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). TCDD treatment degrades AhR expression through activation of the AhR signaling pathway, however there were no survival differences observed in MG-63 cells. There were concomitant elevations of cyclooxygenase-2 and receptor activator of nuclear factor-κB ligand secretion from MG-63 cells upon TCDD treatment on a protein and mRNA level at 24 and 72 h. In addition, TCDD treatment also increases the production of prostaglandin E2 on MG-63 cells, and induces the expression of chemokine receptor CXCR4. However, CXCL12 production was not altered in MG-63 cells when stimulated with TCDD. The AhR antagonist CH-223191, blocks the effects on TCDD-induced RANKL, COX-2, PGE2 and CXCR4 changes. In conclusion, these findings suggest that AhR signal therapy should be further explored as a therapeutic option for the treatment of osteosarcoma.
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Copy and paste a formatted citation
Spandidos Publications style
Yang S, Wu C, Tu Y, Huang S and Chou P: Exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin increases the activation of aryl hydrocarbon receptor and is associated with the aggressiveness of osteosarcoma MG-63 osteoblast-like cells. Oncol Lett 16: 3849-3857, 2018.
APA
Yang, S., Wu, C., Tu, Y., Huang, S., & Chou, P. (2018). Exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin increases the activation of aryl hydrocarbon receptor and is associated with the aggressiveness of osteosarcoma MG-63 osteoblast-like cells. Oncology Letters, 16, 3849-3857. https://doi.org/10.3892/ol.2018.9098
MLA
Yang, S., Wu, C., Tu, Y., Huang, S., Chou, P."Exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin increases the activation of aryl hydrocarbon receptor and is associated with the aggressiveness of osteosarcoma MG-63 osteoblast-like cells". Oncology Letters 16.3 (2018): 3849-3857.
Chicago
Yang, S., Wu, C., Tu, Y., Huang, S., Chou, P."Exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin increases the activation of aryl hydrocarbon receptor and is associated with the aggressiveness of osteosarcoma MG-63 osteoblast-like cells". Oncology Letters 16, no. 3 (2018): 3849-3857. https://doi.org/10.3892/ol.2018.9098
Copy and paste a formatted citation
x
Spandidos Publications style
Yang S, Wu C, Tu Y, Huang S and Chou P: Exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin increases the activation of aryl hydrocarbon receptor and is associated with the aggressiveness of osteosarcoma MG-63 osteoblast-like cells. Oncol Lett 16: 3849-3857, 2018.
APA
Yang, S., Wu, C., Tu, Y., Huang, S., & Chou, P. (2018). Exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin increases the activation of aryl hydrocarbon receptor and is associated with the aggressiveness of osteosarcoma MG-63 osteoblast-like cells. Oncology Letters, 16, 3849-3857. https://doi.org/10.3892/ol.2018.9098
MLA
Yang, S., Wu, C., Tu, Y., Huang, S., Chou, P."Exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin increases the activation of aryl hydrocarbon receptor and is associated with the aggressiveness of osteosarcoma MG-63 osteoblast-like cells". Oncology Letters 16.3 (2018): 3849-3857.
Chicago
Yang, S., Wu, C., Tu, Y., Huang, S., Chou, P."Exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin increases the activation of aryl hydrocarbon receptor and is associated with the aggressiveness of osteosarcoma MG-63 osteoblast-like cells". Oncology Letters 16, no. 3 (2018): 3849-3857. https://doi.org/10.3892/ol.2018.9098
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