Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
September-2018 Volume 16 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
September-2018 Volume 16 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Ailanthone induces autophagic and apoptotic cell death in human promyelocytic leukemia HL‑60 cells

  • Authors:
    • Cheng Wei
    • Chuanrong Chen
    • Yuxin Cheng
    • Lin Zhu
    • Yu Wang
    • Can Luo
    • Yang He
    • Zhiming Yang
    • Zhaoning Ji
  • View Affiliations / Copyright

    Affiliations: The Cancer Center, Yijishan Hospital of Wannan Medical College, Wuhu, Anhui 241001, P.R. China, Department of Oncology, Wuhu No. 2 People's Hospital, Wuhu, Anhui 241001, P.R. China, Department of Oncology, Wannan Medical College, Wuhu, Anhui 241003, P.R. China
    Copyright: © Wei et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3569-3576
    |
    Published online on: July 6, 2018
       https://doi.org/10.3892/ol.2018.9101
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Ailanthone, which is extracted from the traditional Chinese medicinal plant Ailanthus altissima, has been thoroughly demonstrated to have anti‑tumor, anti‑HIV, anti‑inflammatory, anti‑malarial, anti‑allergic and anti‑microbial activities. However, the anti‑proliferative effects of ailanthone on HL‑60 cells and potential mechanisms underlying those effects have not been reported. In the present study, we demonstrated the potent cytotoxicity of ailanthone against HL‑60 cells. Annexin V‑APC/7‑ADD staining assay indicated that ailanthone increased the number of apoptotic cells in a dose‑dependent manner. PI staining showed that ailanthone increased the percentage of G0/G1‑phase cells in a dose‑dependent manner. Acridine orange staining suggested that ailanthone induced the formation of acidic vesicular organelles in HL‑60 cells and pretreatment with BaF‑A1 could attenuate this process. Western blotting showed that ailanthone up‑regulated the protein expression levels of beclin‑1 and LC3‑II and down‑regulated those of LC3‑I and p62 in a dose‑dependent manner. Use of BaF‑A1 showed that the anti‑proliferative effects of ailanthone on HL‑60 cells may be partly attributable to the induction of autophagy‑mediated apoptosis by MTT assay and annexin V‑APC/7‑ADD staining assay.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Vardiman JW, Thiele J, Arber DA, Brunning RD, Borowitz MJ, Porwit A, Harris NL, Le Beau MM, Hellström-Lindberg E, Tefferi A and Bloomfield CD: The 2008 revision of the World Health Organization (WHO) classification of myeloid neoplasms and acute leukemia: Rationale and important changes. Blood. 114:937–951. 2009. View Article : Google Scholar : PubMed/NCBI

2 

Routledge DJ and Bloor AJ: Recent advances in therapy of chronic lymphocytic leukaemia. Br J Haematol. 174:351–367. 2016. View Article : Google Scholar : PubMed/NCBI

3 

Wang Y, Wang WJ, Su C, Zhang DM, Xu LP, He RR, Wang L, Zhang J, Zhang XQ and Ye WC: Cytotoxic quassinoids from Ailanthus altissima. Bioorg Med Chem Lett. 23:654–657. 2013. View Article : Google Scholar : PubMed/NCBI

4 

kundu P and Laskar S: A brief resume on the genus Ailanthus: Chemical and pharmacological aspects. Phytochem Rev. 9:pp379–412. 2010. View Article : Google Scholar

5 

Okunade AL, Bikoff RE, Casper SJ, Oksman A, Goldberg DE and Lewis WH: Antiplasmodial activity of extracts and quassinoids isolated from seedlings of Ailanthus altissima (Simaroubaceae). Phytother Res. 17:675–677. 2003. View Article : Google Scholar : PubMed/NCBI

6 

Fukamiya N, Lee KH, Muhammad I, Murakami C, Okano M, Harvey I and Pelletier J: Structure-activity relationships of quassinoids for eukaryotic protein synthesis. Cancer Lett. 220:37–48. 2005. View Article : Google Scholar : PubMed/NCBI

7 

Rosati A, Quaranta E, Ammirante M, Turco MC, Leone A and De Feo V: Quassinoids can induce mitochondrial membrane depolarisation and caspase 3 activation in human cells. Cell Death Differ. 11 Suppl 2:S216–S218. 2004. View Article : Google Scholar : PubMed/NCBI

8 

Yang XL, Yuan YL, Zhang DM, Li F and Ye WC: Shinjulactone O, a new quassinoid from the root bark of Ailanthus altissima. Nat Prod Res. 28:1432–1437. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Zhuo Z, Hu J, Yang X, Chen M, Lei X, Deng L, Yao N, Peng Q, Chen Z, Ye W and Zhang D: Ailanthone inhibits Huh7 cancer cell growth via cell cycle arrest and apoptosis in vitro and in vivo. Sci Rep. 5:161852015. View Article : Google Scholar : PubMed/NCBI

10 

Evan GI and Vousden KH: Proliferation, cell cycle and apoptosis in cancer. Nature. 411:342–348. 2001. View Article : Google Scholar : PubMed/NCBI

11 

Kondo S, Tanaka Y, Kondo Y, Hitomi M, Barnett GH, Ishizaka Y, Liu J, Haqqi T, Nishiyama A, Villeponteau B, et al: Antisense telomerase treatment: Induction of two distinct pathways, apoptosis and differentiation. FASEB J. 12:801–811. 1998. View Article : Google Scholar : PubMed/NCBI

12 

Portt L, Norman G, Clapp C, Greenwood M and Greenwood MT: Anti-apoptosis and cell survival: A review. Biochim Biophys Acta. 1813:238–259. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Klionsky DJ, Abdelmohsen K, Abe A, Abedin MJ, Abeliovich H, Arozena Acevedo A, Adachi H, Adams CM, Adams PD, Adeli K, et al: Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy. 12:1–222. 2016. View Article : Google Scholar : PubMed/NCBI

14 

Paglin S, Hollister T, Delohery T, Hackett N, McMahill M, Sphicas E, Domingo D and Yahalom J: A novel response of cancer cells to radiation involves autophagy and formation of acidic vesicles. Cancer Res. 61:439–444. 2001.PubMed/NCBI

15 

Kondo Y, Kanzawa T, Sawaya R and Kondo S: The role of autophagy in cancer development and response to therapy. Nat Rev Cancer. 5:726–734. 2005. View Article : Google Scholar : PubMed/NCBI

16 

Klionsky DJ and Emr SD: Autophagy as a regulated pathway of cellular degradation. Science. 290:1717–1721. 2000. View Article : Google Scholar : PubMed/NCBI

17 

Kanzawa T, Kondo Y, Ito H, Kondo S and Germano I: Induction of autophagic cell death in malignant glioma cells by arsenic trioxide. Cancer Res. 63:2103–2108. 2003.PubMed/NCBI

18 

Tsang CK, Qi H, Liu LF and Zheng XF: Targeting mammalian target of rapamycin (mTOR) for health and diseases. Drug Discov Today. 12:112–124. 2007. View Article : Google Scholar : PubMed/NCBI

19 

Xia Z, Zhang H, Xu D, Lao Y, Fu W, Tan H, Cao P, Yang L and Xu H: Xanthones from the leaves of garcinia cowa induce cell cycle arrest, apoptosis, and autophagy in cancer cells. Molecules. 20:11387–11399. 2015. View Article : Google Scholar : PubMed/NCBI

20 

Schwartz GK and Shah MA: Targeting the cell cycle: A new approach to cancer therapy. J Clin Oncol. 23:9408–9421. 2005. View Article : Google Scholar : PubMed/NCBI

21 

Pardee AB: A restriction point for control of normal animal cell proliferation. Proc Natl Acad Sci USA. 71:1286–1290. 1974. View Article : Google Scholar : PubMed/NCBI

22 

Sherr CJ: The Pezcoller lecture: Cancer cell cycles revisited. Cancer Res. 60:3689–3695. 2000.PubMed/NCBI

23 

Watson NC, Di YM, Orr MS, Fornari FA Jr, Randolph JK, Magnet KJ, Jain PT and Gewirtz DA: Influence of ionizing radiation on proliferation, c-myc expression and the induction of apoptotic cell death in two breast tumour cell lines differing in p53 status. Int J Radiat Biol. 72:547–559. 1997. View Article : Google Scholar : PubMed/NCBI

24 

Lavhale MS, Kumar S, Mishra SH and Sitasawad SL: A novel triterpenoid isolated from the root bark of Ailanthus excelsa Roxb (Tree of Heaven), AECHL-1 as a potential anti-cancer agent. PLoS One. 4:e53652009. View Article : Google Scholar : PubMed/NCBI

25 

Wang ZH, Peng ZL, Duan ZL and Liu H: Expression and clinical significance of autophagy gene Beclin 1 in cervical squamous cell carcinoma. Sichuan Da Xue Xue Bao Yi Xue Ban. 37:860–863. 2006.(In Chinese). PubMed/NCBI

26 

Xu MY, Lee DH, Joo EJ, Son KH and Kim YS: Akebia saponin PA induces autophagic and apoptotic cell death in AGS human gastric cancer cells. Food Chem Toxicol. 59:703–708. 2013. View Article : Google Scholar : PubMed/NCBI

27 

Mizushima N, Ohsumi Y and Yoshimori T: Autophagosome formation in mammalian cells. Cell Struct Funct. 27:421–429. 2002. View Article : Google Scholar : PubMed/NCBI

28 

Česen MH, Pegan K, Spes A and Turk B: Lysosomal pathways to cell death and their therapeutic applications. Exp Cell Res. 318:1245–1251. 2012. View Article : Google Scholar : PubMed/NCBI

29 

Yamamoto A, Tagawa Y, Yoshimori T, Moriyama Y, Masaki R and Tashiro Y: Bafilomycin A1 prevents maturation of autophagic vacuoles by inhibiting fusion between autophagosomes and lysosomes in rat hepatoma cell line, H-4-II-E cells. Cell Struct Funct. 23:33–42. 1998. View Article : Google Scholar : PubMed/NCBI

30 

Klionsky DJ, Elazar Z, Seglen PO and Rubinsztein DC: Does bafilomycin A1 block the fusion of autophagosomes with lysosomes? Autophagy. 4:849–850. 2008. View Article : Google Scholar : PubMed/NCBI

31 

Mehdi S: Cell-penetrating inhibitors of calpain. Trends Biochem Sci. 16:150–153. 1991. View Article : Google Scholar : PubMed/NCBI

32 

Seglen PO, Grinde B and Solheim AE: Inhibition of the lysosomal pathway of protein degradation in isolated rat hepatocytes by ammonia, methylamine, chloroquine and leupeptin. Eur J Biochem. 95:215–225. 1979. View Article : Google Scholar : PubMed/NCBI

33 

Phadwal K, Watson AS and Simon AK: Tightrope act: Autophagy in stem cell renewal, differentiation, proliferation, and aging. Cell Mol Life Sci. 70:89–103. 2013. View Article : Google Scholar : PubMed/NCBI

34 

Gozuacik D and Kimchi A: Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 23:2891–2906. 2004. View Article : Google Scholar : PubMed/NCBI

35 

Tanida I, Ueno T and Kominami E: LC3 conjugation system in mammalian autophagy. Int J Biochem Cell Biol. 36:2503–2518. 2004. View Article : Google Scholar : PubMed/NCBI

36 

Seaman MN, Marcusson EG, Cereghino JL and Emr SD: Endosome to Golgi retrieval of the vacuolar protein sorting receptor, Vps10p, requires the function of the VPS29, VPS30, and VPS35 gene products. J Cell Biol. 137:79–92. 1997. View Article : Google Scholar : PubMed/NCBI

37 

Pattingre S, Tassa A, Qu X, Garuti R, Liang XH, Mizushima N, Packer M, Schneider MD and Levine B: Bcl-2 antiapoptotic proteins inhibit Beclin 1-dependent autophagy. Cell. 122:927–939. 2005. View Article : Google Scholar : PubMed/NCBI

38 

Mizushima N and Komatsu M: Autophagy: Renovation of cells and tissues. Cell. 147:728–741. 2011. View Article : Google Scholar : PubMed/NCBI

39 

Yang P, Sun D and Jiang F: Ailanthone promotes human vestibular schwannoma cells apoptosis and autophagy by down-regulation of miR-21. Oncol Res. Jan 3–2018.(Epub ahead of print). View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Wei C, Chen C, Cheng Y, Zhu L, Wang Y, Luo C, He Y, Yang Z and Ji Z: Ailanthone induces autophagic and apoptotic cell death in human promyelocytic leukemia HL‑60 cells. Oncol Lett 16: 3569-3576, 2018.
APA
Wei, C., Chen, C., Cheng, Y., Zhu, L., Wang, Y., Luo, C. ... Ji, Z. (2018). Ailanthone induces autophagic and apoptotic cell death in human promyelocytic leukemia HL‑60 cells. Oncology Letters, 16, 3569-3576. https://doi.org/10.3892/ol.2018.9101
MLA
Wei, C., Chen, C., Cheng, Y., Zhu, L., Wang, Y., Luo, C., He, Y., Yang, Z., Ji, Z."Ailanthone induces autophagic and apoptotic cell death in human promyelocytic leukemia HL‑60 cells". Oncology Letters 16.3 (2018): 3569-3576.
Chicago
Wei, C., Chen, C., Cheng, Y., Zhu, L., Wang, Y., Luo, C., He, Y., Yang, Z., Ji, Z."Ailanthone induces autophagic and apoptotic cell death in human promyelocytic leukemia HL‑60 cells". Oncology Letters 16, no. 3 (2018): 3569-3576. https://doi.org/10.3892/ol.2018.9101
Copy and paste a formatted citation
x
Spandidos Publications style
Wei C, Chen C, Cheng Y, Zhu L, Wang Y, Luo C, He Y, Yang Z and Ji Z: Ailanthone induces autophagic and apoptotic cell death in human promyelocytic leukemia HL‑60 cells. Oncol Lett 16: 3569-3576, 2018.
APA
Wei, C., Chen, C., Cheng, Y., Zhu, L., Wang, Y., Luo, C. ... Ji, Z. (2018). Ailanthone induces autophagic and apoptotic cell death in human promyelocytic leukemia HL‑60 cells. Oncology Letters, 16, 3569-3576. https://doi.org/10.3892/ol.2018.9101
MLA
Wei, C., Chen, C., Cheng, Y., Zhu, L., Wang, Y., Luo, C., He, Y., Yang, Z., Ji, Z."Ailanthone induces autophagic and apoptotic cell death in human promyelocytic leukemia HL‑60 cells". Oncology Letters 16.3 (2018): 3569-3576.
Chicago
Wei, C., Chen, C., Cheng, Y., Zhu, L., Wang, Y., Luo, C., He, Y., Yang, Z., Ji, Z."Ailanthone induces autophagic and apoptotic cell death in human promyelocytic leukemia HL‑60 cells". Oncology Letters 16, no. 3 (2018): 3569-3576. https://doi.org/10.3892/ol.2018.9101
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team