Combined effects of octreotide and cisplatin on the proliferation of side population cells from anaplastic thyroid cancer cell lines

  • Authors:
    • Zhilan Li
    • Xiudi Jiang
    • Peihong Chen
    • Xuebing Wu
    • Aihua Duan
    • Yiyu Qin
  • View Affiliations

  • Published online on: July 10, 2018     https://doi.org/10.3892/ol.2018.9105
  • Pages: 4033-4042
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Anaplastic thyroid cancer (ATC) represents the most aggressive subtype of thyroid cancer and has a poor prognosis. In addition to surgery, chemotherapy is an important treatment for ATC; however, the therapeutic effects of current chemotherapies for ATC are not particularly promising. There is a high proportion of side population (SP) cells in ATC, which may be a reason for its drug resistance. In the present study, the antitumor activities of combined octreotide (OCT) and cisplatin (DDP) on the proliferation and apoptosis of ATC SP cells were evaluated. First, SP cells from 8305C and BHT101 cell lines were detected and sorted. Following in vitro culture for 1 week, cluster of differentiation (CD)44, CD133, ATP‑binding cassette (ABC) subfamily B member 1 (ABCB1), ABC subfamily G member 2 (ABCG2) and somatostatin receptor expression was detected to characterize the SP cells. An MTT assay was performed to investigate the combined effects on 8305C‑SP cell proliferation in vitro, and a mouse model was used to investigate the combined effects on 8305C‑SP cell proliferation in vivo. Annexin V/propidium iodide staining was used to investigate the combined effects on 8305C‑SP cell apoptosis. Chemotherapeutic drug resistance‑associated protein expression and apoptosis‑associated protein expression were also detected following combined treatment. As a result, SP cells were identified in 8305C and BHT101 cells, and the proportion of 8305C‑SP cells was increased compared with that of BTH101‑SP cells. SP cells have enhanced proliferation, tumorigenicity and drug resistance compared with main population cells. The combined treatment of OCT with DDP suppressed the proliferation of 8305C‑SP cells in vitro and in vivo, and induced 8305C‑SP cell apoptosis. Combined treatment decreased the ABCB1 and ABCG2 expression by SP cells and activated mitochondrial apoptotic signaling, resulting in cell apoptosis. In conclusion, these data support the hypothesis that combined treatment with OCT and DDP induces ATC cell apoptosis and suppresses cell proliferation. These data provide a theoretical basis for further combined chemotherapy clinical applications.
View Figures
View References

Related Articles

Journal Cover

September-2018
Volume 16 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Li Z, Jiang X, Chen P, Wu X, Duan A and Qin Y: Combined effects of octreotide and cisplatin on the proliferation of side population cells from anaplastic thyroid cancer cell lines. Oncol Lett 16: 4033-4042, 2018
APA
Li, Z., Jiang, X., Chen, P., Wu, X., Duan, A., & Qin, Y. (2018). Combined effects of octreotide and cisplatin on the proliferation of side population cells from anaplastic thyroid cancer cell lines. Oncology Letters, 16, 4033-4042. https://doi.org/10.3892/ol.2018.9105
MLA
Li, Z., Jiang, X., Chen, P., Wu, X., Duan, A., Qin, Y."Combined effects of octreotide and cisplatin on the proliferation of side population cells from anaplastic thyroid cancer cell lines". Oncology Letters 16.3 (2018): 4033-4042.
Chicago
Li, Z., Jiang, X., Chen, P., Wu, X., Duan, A., Qin, Y."Combined effects of octreotide and cisplatin on the proliferation of side population cells from anaplastic thyroid cancer cell lines". Oncology Letters 16, no. 3 (2018): 4033-4042. https://doi.org/10.3892/ol.2018.9105