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Overexpression of SMARCA2 or CAMK2D is associated with cisplatin resistance in human epithelial ovarian cancer

  • Authors:
    • Xiaoli Xu
    • Zhiguo Zheng
    • Lanlan Jia
    • Shasha Suo
    • Bowen Liu
    • Tianning Shao
    • Qinqing Tu
    • Yuejin Hua
    • Hong Xu
  • View Affiliations / Copyright

    Affiliations: Institute of Nuclear‑Agricultural Sciences, Zhejiang University, Hangzhou, Zhejiang 310029, P.R. China, Institute of Zhejiang Cancer Research, Zhejiang Cancer Hospital, Hangzhou, Zhejiang 310022, P.R. China
    Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3796-3804
    |
    Published online on: July 10, 2018
       https://doi.org/10.3892/ol.2018.9109
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Abstract

Ovarian cancer is one of the most lethal types of gynecological cancer. Drug resistance is a major underlying cause of treatment failure, which has lead to continued poor mortality and morbidity rates in patients. In the present study, highly sensitive transcriptome sequencing was performed to systematically identify differentially expressed mRNAs in cisplatin‑sensitive (A2780) and ‑resistant (A2780‑DR) cells. Calcium/calmodulin dependent protein kinase IIδ (CAMK2D) and SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily A member 2 (SMARCA2) were identified as exhibiting increased expression in cisplatin‑resistant cells. Overexpression of either SMARCA2 or CAMK2D led to a significant increase in the survival rates of A2780 and SKVO3 cells following cisplatin treatment. To further verify the contribution of these two genes in the development of drug resistance, the RNA levels in tissues with different recurrence‑free survival (RFS) rates were compared. An increased mRNA level of CAMK2D was detected in samples with shorter RFS rates. An apoptosis assay revealed that overexpression of SMARCA2 or CAMK2D increased the resistance of ovarian cancer cells to cisplatin, as indicated by the decreased apoptotic cell populations. The levels of these two genes also affected the cell cycle and apoptosis‑associated protein expression. Quantitative proteomic analyses revealed that overexpression of SMARCA2 or CAMK2D influences multiple metabolism and cancer‑associated signaling pathways, which are critical for responses to cisplatin treatment and drug resistance development.
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Copy and paste a formatted citation
Spandidos Publications style
Xu X, Zheng Z, Jia L, Suo S, Liu B, Shao T, Tu Q, Hua Y and Xu H: Overexpression of SMARCA2 or CAMK2D is associated with cisplatin resistance in human epithelial ovarian cancer. Oncol Lett 16: 3796-3804, 2018.
APA
Xu, X., Zheng, Z., Jia, L., Suo, S., Liu, B., Shao, T. ... Xu, H. (2018). Overexpression of SMARCA2 or CAMK2D is associated with cisplatin resistance in human epithelial ovarian cancer. Oncology Letters, 16, 3796-3804. https://doi.org/10.3892/ol.2018.9109
MLA
Xu, X., Zheng, Z., Jia, L., Suo, S., Liu, B., Shao, T., Tu, Q., Hua, Y., Xu, H."Overexpression of SMARCA2 or CAMK2D is associated with cisplatin resistance in human epithelial ovarian cancer". Oncology Letters 16.3 (2018): 3796-3804.
Chicago
Xu, X., Zheng, Z., Jia, L., Suo, S., Liu, B., Shao, T., Tu, Q., Hua, Y., Xu, H."Overexpression of SMARCA2 or CAMK2D is associated with cisplatin resistance in human epithelial ovarian cancer". Oncology Letters 16, no. 3 (2018): 3796-3804. https://doi.org/10.3892/ol.2018.9109
Copy and paste a formatted citation
x
Spandidos Publications style
Xu X, Zheng Z, Jia L, Suo S, Liu B, Shao T, Tu Q, Hua Y and Xu H: Overexpression of SMARCA2 or CAMK2D is associated with cisplatin resistance in human epithelial ovarian cancer. Oncol Lett 16: 3796-3804, 2018.
APA
Xu, X., Zheng, Z., Jia, L., Suo, S., Liu, B., Shao, T. ... Xu, H. (2018). Overexpression of SMARCA2 or CAMK2D is associated with cisplatin resistance in human epithelial ovarian cancer. Oncology Letters, 16, 3796-3804. https://doi.org/10.3892/ol.2018.9109
MLA
Xu, X., Zheng, Z., Jia, L., Suo, S., Liu, B., Shao, T., Tu, Q., Hua, Y., Xu, H."Overexpression of SMARCA2 or CAMK2D is associated with cisplatin resistance in human epithelial ovarian cancer". Oncology Letters 16.3 (2018): 3796-3804.
Chicago
Xu, X., Zheng, Z., Jia, L., Suo, S., Liu, B., Shao, T., Tu, Q., Hua, Y., Xu, H."Overexpression of SMARCA2 or CAMK2D is associated with cisplatin resistance in human epithelial ovarian cancer". Oncology Letters 16, no. 3 (2018): 3796-3804. https://doi.org/10.3892/ol.2018.9109
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