Effect of As2O3 on colorectal CSCs stained with ALDH1 in primary cell culture in vitro

Ning,Ke; Ning,Wenlong; Ning,Xiaoting; Wang,Xueyan; Zhou,Fei

doi:10.3892/ol.2018.9110

September-2018 Volume 16 Issue 3

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September-2018 Volume 16 Issue 3

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Article

Effect of As2O3 on colorectal CSCs stained with ALDH1 in primary cell culture in vitro

Authors:
- Ke Ning
- Wenlong Ning
- Xiaoting Ning
- Xueyan Wang
- Fei Zhou
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Affiliations: Department of Internal Neurology, Baotou Medical School, Inner Mongolia Medical University, Inner Mongolia 014000, P.R. China, Department of Emergency, The First Hospital of Qiqihar, Qiqihar, Heilongjiang 161005, P.R. China, Department of ECG Room of Second Ward, The First Hospital of Qiqihar, Qiqihar, Heilongjiang 161005, P.R. China, Department of Public Health, The First Hospital of Qiqihar, Qiqihar, Heilongjiang 161005, P.R. China, Second Department of Breast Surgery, The First Hospital of Qiqihar, Qiqihar, Heilongjiang 161005, P.R. China
Pages: 4008-4012
|
Published online on: July 10, 2018

https://doi.org/10.3892/ol.2018.9110
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Abstract

Colorectal carcinoma (CRC) is one of the most common types of malignant tumor in humans, and its morbidity is on the increase in economically transitioning countries. Due to its high toxicity, the use of the Chinese Traditional Medicine arsenic (As2O3) is limited. However, certain studies have reported that As2O3 induces differentiation of tumor cells, promotes tumor cell apoptosis and inhibits tumor cell proliferation, although the number of studies on the effects of As2O3 on cancer stem cells (CSCs) of CRC is limited. In order to research the effects of different concentrations of As2O3 on CRC cells and colorectal CSCs in vitro, aldehyde dehydrogenase 1 (ALDH1) was sued to stain the cytoplasm of colorectal CSCs and DAPI was used to stain the nuclei of all tumor cells. Through observing the effect of different concentrations of As2O3 on CRC cells and colorectal CSCs, it was demonstrated that a sufficient concentration of As2O3 clearly inhibited the conversion from colorectal CSCs to CRC cells and increased the density of CSCs.

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