Open Access

CREB1, a direct target of miR-122, promotes cell proliferation and invasion in bladder cancer

  • Authors:
    • Lihua Guo
    • Min Yin
    • Yixuan Wang
  • View Affiliations

  • Published online on: July 10, 2018     https://doi.org/10.3892/ol.2018.9118
  • Pages: 3842-3848
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Abstract

Bladder cancer (BC) is a prevalent cancer, which arises from the epithelial lining of the urinary bladder. CAMP-response element binding protein (CREB1) acts as a transcription factor, which regulates cell transcription through phosphorylation and dephosphorylation. The purpose of this study was to explore how miR-122 worked in BC on cell proliferation and invasion. RT-qPCR was applied to evaluate the mRNA levels of CREB1 and miR-122 in BC. CCK-8 and Transwell assays were employed to determine the migratory and invasive abilities. Dual luciferase reporter assay was applied to verify miR-122 targeting CREB1 in BC. CREB1 was upregulated in bladder tissues and T24, UM-UC-3 and J82 cells, while miR-122 upregulated and had negative correlation with CREB1. Moreover, knockdown of CREB1 inhibited cell proliferative and invasive capacities. In addition, CREB1 was directly targeted by miR-122 in BC and regulated its expression. We discovered that CREB1 could reverse partially the function of miR-122 on cell proliferation and invasion. CREB1 was mediated by miR-122, and regulated cell proliferation and invasiveness. The newly identified miR-122/CREB1 axis provides novel insight into the pathogenesis of BC.
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September-2018
Volume 16 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Guo L, Yin M and Wang Y: CREB1, a direct target of miR-122, promotes cell proliferation and invasion in bladder cancer. Oncol Lett 16: 3842-3848, 2018
APA
Guo, L., Yin, M., & Wang, Y. (2018). CREB1, a direct target of miR-122, promotes cell proliferation and invasion in bladder cancer. Oncology Letters, 16, 3842-3848. https://doi.org/10.3892/ol.2018.9118
MLA
Guo, L., Yin, M., Wang, Y."CREB1, a direct target of miR-122, promotes cell proliferation and invasion in bladder cancer". Oncology Letters 16.3 (2018): 3842-3848.
Chicago
Guo, L., Yin, M., Wang, Y."CREB1, a direct target of miR-122, promotes cell proliferation and invasion in bladder cancer". Oncology Letters 16, no. 3 (2018): 3842-3848. https://doi.org/10.3892/ol.2018.9118