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Article Open Access

miR-329 regulates the sensitivity of 5-FU in chemotherapy of colorectal cancer by targeting E2F1

  • Authors:
    • Jie Yin
    • Xiping Shen
    • Mei Li
    • Fangying Ni
    • Li Xu
    • Hua Lu
  • View Affiliations / Copyright

    Affiliations: Department of Anorectal Surgery, The First People's Hospital of Wujiang District, Suzhou, Jiangsu 215200, P.R. China, Department of Anorectal Surgery, The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410000, P.R. China, Scientific Education Section, The First People's Hospital of Wujiang District, Suzhou, Jiangsu 215200, P.R. China
    Copyright: © Yin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3587-3592
    |
    Published online on: July 11, 2018
       https://doi.org/10.3892/ol.2018.9121
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Abstract

Colorectal cancer (CRC) is a common digestive system malignancy with high morbidity and mortality. Accumulating studies have shown that miRNAs play a critical role in the progression of CRC. Here, we explored the effect of miR-329 and its target gene on the sensitivity of 5-fluorouracil (5-FU) in the chemotherapy of CRC. RT-qPCR was utilized to determine the expression of miR-329 in cancer tissues, adjacent tissues and cells. CCK-8 and Transwell assays were introduced to detect the role of miR-329 overexpression in cell viability and invasion. Luciferase reporter assay was performed to verify that E2F1 was a direct target of miR-329. Protein expression of E2F1 was accessed by western blot analysis. The expression level of miR-329 was decreased in CRC tissues and tumor tissues at stage III+IV with lymph node metastasis, and the patients' total survival time was positively associated with the expression of miR-329. Overexpression of miR-329 significantly attenuated the viability and invasiveness of tumor cells. The viability of drug-resistant cells was markedly higher than that of non-resistant cells under the same dose of 5-FU treatment. The expression of miR-329 in tumor cells was negatively associated with drug sensitivity. Luciferase reporter assay showed that E2F1 was the direct target of miR-329. Besides, the expression of E2F1 protein in drug-resistant cells was remarkably higher than that in the non-resistant cells, while the overexpression of miR-329 significantly decreased the expression of E2F1 protein. E2F1 overexpression increased cell viability, but overexpression of both E2F1 and miR-329 in turn decreased cell viability. miR-329 expression is reduced in CRC, and overexpression of miR-329 promotes the sensitivity of 5-FU in the chemotherapy of CRC by degrading the target gene E2F1.
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Copy and paste a formatted citation
Spandidos Publications style
Yin J, Shen X, Li M, Ni F, Xu L and Lu H: miR-329 regulates the sensitivity of 5-FU in chemotherapy of colorectal cancer by targeting E2F1. Oncol Lett 16: 3587-3592, 2018.
APA
Yin, J., Shen, X., Li, M., Ni, F., Xu, L., & Lu, H. (2018). miR-329 regulates the sensitivity of 5-FU in chemotherapy of colorectal cancer by targeting E2F1. Oncology Letters, 16, 3587-3592. https://doi.org/10.3892/ol.2018.9121
MLA
Yin, J., Shen, X., Li, M., Ni, F., Xu, L., Lu, H."miR-329 regulates the sensitivity of 5-FU in chemotherapy of colorectal cancer by targeting E2F1". Oncology Letters 16.3 (2018): 3587-3592.
Chicago
Yin, J., Shen, X., Li, M., Ni, F., Xu, L., Lu, H."miR-329 regulates the sensitivity of 5-FU in chemotherapy of colorectal cancer by targeting E2F1". Oncology Letters 16, no. 3 (2018): 3587-3592. https://doi.org/10.3892/ol.2018.9121
Copy and paste a formatted citation
x
Spandidos Publications style
Yin J, Shen X, Li M, Ni F, Xu L and Lu H: miR-329 regulates the sensitivity of 5-FU in chemotherapy of colorectal cancer by targeting E2F1. Oncol Lett 16: 3587-3592, 2018.
APA
Yin, J., Shen, X., Li, M., Ni, F., Xu, L., & Lu, H. (2018). miR-329 regulates the sensitivity of 5-FU in chemotherapy of colorectal cancer by targeting E2F1. Oncology Letters, 16, 3587-3592. https://doi.org/10.3892/ol.2018.9121
MLA
Yin, J., Shen, X., Li, M., Ni, F., Xu, L., Lu, H."miR-329 regulates the sensitivity of 5-FU in chemotherapy of colorectal cancer by targeting E2F1". Oncology Letters 16.3 (2018): 3587-3592.
Chicago
Yin, J., Shen, X., Li, M., Ni, F., Xu, L., Lu, H."miR-329 regulates the sensitivity of 5-FU in chemotherapy of colorectal cancer by targeting E2F1". Oncology Letters 16, no. 3 (2018): 3587-3592. https://doi.org/10.3892/ol.2018.9121
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