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Article

Upregulation of gastric adenocarcinoma predictive long intergenic non‑coding RNA promotes progression and predicts poor prognosis in perihilar cholangiocarcinoma

  • Authors:
    • Xin‑Ping Wang
    • Jing Song
    • Gui‑Ting Liu
    • Jian‑Jun Wang
    • Hai‑Feng Guo
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Hongqi Hospital of Mudanjiang Medical College, Mudanjiang, Heilongjiang 157011, P.R. China, Department of Urology, Hongqi Hospital of Mudanjiang Medical College, Mudanjiang, Heilongjiang 157011, P.R. China, Department of Thoracic Surgery, Hongqi Hospital of Mudanjiang Medical College, Mudanjiang, Heilongjiang 157011, P.R. China
  • Pages: 3964-3972
    |
    Published online on: July 11, 2018
       https://doi.org/10.3892/ol.2018.9137
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Abstract

Perihilar cholangiocarcinoma (PHCC) is one of the most aggressive and complex types of cancer with a poor survival. Despite advances in PHCC diagnosis and treatment, the biology of this tumor remains poorly understood. Recent studies have suggested long non‑coding RNAs (lncRNAs) as crucial determinants of cancer progression. However, the role of lncRNAs in PHCC is rarely reported and the function of gastric adenocarcinoma predictive long intergenic non‑coding RNA (GAPLINC) in PHCC has yet to be elucidated. The present study observed a significant upregulation of GAPLINC in PHCC cell lines and clinical specimens (P<0.05). Furthermore, by comparing clinicopathological characteristics with expression data, high GAPLINC expression was revealed to be associated with the T stage (P=0.013), N stage (P<0.001) and Tumor‑Node‑Metastasis stage (P<0.001) of PHCC. Furthermore, Kaplan‑Meier analysis demonstrated that GAPLINC expression was associated with poor overall survival and progression‑free survival rates in PHCC. Furthermore, univariate and multivariate COX regression analyses identified high GAPLINC expression as a risk factor of a poor prognosis in PHCC. GAPLINC upregulation promoted the migration and invasion of PHCC cells in Transwell and Matrigel assays, respectively, while GAPLINC deficiency inhibited PHCC cell metastasis. Furthermore, PHCC cells with GAPLINC overexpression exhibited markedly increased proliferation ability in a Cell Counting kit‑8 assay. However, GAPLINC interference significantly suppressed cell proliferation. In conclusion, GAPLINC may promote PHCC progression and may serve as a potential prognostic marker and therapeutic target of PHCC.
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1 

Razumilava N and Gores GJ: Cholangiocarcinoma. Lancet. 383:2168–2179. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Khan SA, Davidson BR, Goldin RD, Heaton N, Karani J, Pereira SP, Rosenberg WM, Tait P, Taylor-Robinson SD, Thillainayagam AV, et al: Guidelines for the diagnosis and treatment of cholangiocarcinoma: An update. Gut. 61:1657–1669. 2012. View Article : Google Scholar : PubMed/NCBI

3 

Zhimin G, Noor H, Jian-Bo Z, Lin W and Jha RK: Advances in diagnosis and treatment of hilar cholangiocarcinoma-a review. Med Sci Monit. 19:648–656. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Patel T: Cholangiocarcinoma-controversies and challenges. Nat Rev Gastroenterol Hepatol. 8:189–200. 2011. View Article : Google Scholar : PubMed/NCBI

5 

Guttman M, Amit I, Garber M, French C, Lin MF, Feldser D, Huarte M, Zuk O, Carey BW, Cassady JP, et al: Chromatin signature reveals over a thousand highly conserved large non-coding RNAs in mammals. Nature. 458:223–227. 2009. View Article : Google Scholar : PubMed/NCBI

6 

ENCODE Project Consortium, . Birney E, Stamatoyannopoulos JA, Dutta A, Guigó R, Gingeras TR, Margulies EH, Weng Z, Snyder M, Dermitzakis ET, et al: Identification and analysis of functional elements in 1% of the human genome by the ENCODE pilot project. Nature. 447:799–816. 2007. View Article : Google Scholar : PubMed/NCBI

7 

Nagano T and Fraser P: No-nonsense functions for long noncoding RNAs. Cell. 145:178–181. 2011. View Article : Google Scholar : PubMed/NCBI

8 

Ponting CP, Oliver PL and Reik W: Evolution and functions of long noncoding RNAs. Cell. 136:629–641. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Li X, Wu Z, Fu X and Han W: lncRNAs: Insights into their function and mechanics in underlying disorders. Mutat Res Rev Mutat Res. 762:1–21. 2014. View Article : Google Scholar : PubMed/NCBI

10 

Cheetham SW, Gruhl F, Mattick JS and Dinger ME: Long noncoding RNAs and the genetics of cancer. Br J Cancer. 108:2419–2425. 2013. View Article : Google Scholar : PubMed/NCBI

11 

Evans JR, Feng FY and Chinnaiyan AM: The bright side of dark matter: lncRNAs in cancer. J Clin Invest. 126:2775–2782. 2016. View Article : Google Scholar : PubMed/NCBI

12 

Serviss JT, Johnsson P and Grander D: An emerging role for long non-coding RNAs in cancer metastasis. Front Genet. 5:2342014. View Article : Google Scholar : PubMed/NCBI

13 

Hu Y, Wang J, Qian J, Kong X, Tang J, Wang Y, Chen H, Hong J, Zou W, Chen Y, et al: Long noncoding RNA GAPLINC regulates CD44-dependent cell invasiveness and associates with poor prognosis of gastric cancer. Cancer Res. 74:6890–6902. 2014. View Article : Google Scholar : PubMed/NCBI

14 

Yang P, Chen T, Xu Z, Zhu H, Wang J and He Z: Long noncoding RNA GAPLINC promotes invasion in colorectal cancer by targeting SNAI2 through binding with PSF and NONO. Oncotarget. 7:42183–42194. 2016.PubMed/NCBI

15 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

16 

Howell M and Valle JW: The role of adjuvant chemotherapy and radiotherapy for cholangiocarcinoma. Best Pract Res Clin Gastroenterol. 29:333–343. 2015. View Article : Google Scholar : PubMed/NCBI

17 

Mourtada-Maarabouni M, Pickard MR, Hedge VL, Farzaneh F and Williams GT: GAS5, a non-protein-coding RNA, controls apoptosis and is downregulated in breast cancer. Oncogene. 28:195–208. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Xing Z, Lin A, Li C, Liang K, Wang S, Liu Y, Park PK, Qin L, Wei Y, Hawke DH, et al: lncRNA directs cooperative epigenetic regulation downstream of chemokine signals. Cell. 159:1110–1125. 2014. View Article : Google Scholar : PubMed/NCBI

19 

Poliseno L, Salmena L, Zhang J, Carver B, Haveman WJ and Pandolfi PP: A coding-independent function of gene and pseudogene mRNAs regulates tumour biology. Nature. 465:1033–1038. 2010. View Article : Google Scholar : PubMed/NCBI

20 

Gupta RA, Shah N, Wang KC, Kim J, Horlings HM, Wong DJ, Tsai MC, Hung T, Argani P, Rinn JL, et al: Long non-coding RNA HOTAIR reprograms chromatin state to promote cancer metastasis. Nature. 464:1071–1076. 2010. View Article : Google Scholar : PubMed/NCBI

21 

Li L, Liu B, Wapinski OL, Tsai MC, Qu K, Zhang J, Carlson JC, Lin M, Fang F, Gupta RA, et al: Targeted disruption of Hotair leads to homeotic transformation and gene derepression. Cell Rep. 5:3–12. 2013. View Article : Google Scholar : PubMed/NCBI

22 

Ji P, Diederichs S, Wang W, Böing S, Metzger R, Schneider PM, Tidow N, Brandt B, Buerger H, Bulk E, et al: MALAT-1, a novel noncoding RNA, and thymosin beta4 predict metastasis and survival in early-stage non-small cell lung cancer. Oncogene. 22:8031–8041. 2003. View Article : Google Scholar : PubMed/NCBI

23 

Gutschner T, Hämmerle M and Diederichs S: MALAT1-a paradigm for long noncoding RNA function in cancer. J Mol Med (Berl). 91:791–801. 2013. View Article : Google Scholar : PubMed/NCBI

24 

Tripathi V, Ellis JD, Shen Z, Song DY, Pan Q, Watt AT, Freier SM, Bennett CF, Sharma A, Bubulya PA, et al: The nuclear-retained noncoding RNA MALAT1 regulates alternative splicing by modulating SR splicing factor phosphorylation. Mol Cell. 39:925–938. 2010. View Article : Google Scholar : PubMed/NCBI

25 

Prensner JR, Iyer MK, Balbin OA, Dhanasekaran SM, Cao Q, Brenner JC, Laxman B, Asangani IA, Grasso CS, Kominsky HD, et al: Transcriptome sequencing across a prostate cancer cohort identifies PCAT-1, an unannotated lincRNA implicated in disease progression. Nat Biotechnol. 29:742–749. 2011. View Article : Google Scholar : PubMed/NCBI

26 

Prensner JR, Chen W, Han S, Iyer MK, Cao Q, Kothari V, Evans JR, Knudsen KE, Paulsen MT, Ljungman M, et al: The long non-coding RNA PCAT-1 promotes prostate cancer cell proliferation through cMyc. Neoplasia. 16:900–908. 2014. View Article : Google Scholar : PubMed/NCBI

27 

Wang WT, Ye H, Wei PP, Han BW, He B, Chen ZH and Chen YQ: LncRNAs H19 and HULC, activated by oxidative stress, promote cell migration and invasion in cholangiocarcinoma through a ceRNA manner. J Hematol Oncol. 9:1172016. View Article : Google Scholar : PubMed/NCBI

28 

Ma SL, Li AJ, Hu ZY, Shang FS and Wu MC: Co-expression of the carbamoyl-phosphate synthase 1 gene and its long non-coding RNA correlates with poor prognosis of patients with intrahepatic cholangiocarcinoma. Mol Med Rep. 12:7915–7926. 2015. View Article : Google Scholar : PubMed/NCBI

29 

Tan X, Huang Z and Li X: Long non-coding RNA MALAT1 interacted with miR-204 to modulates human Hilar cholangiocarcinoma proliferation, migration and invasion by targeting CXCR4. J Cell Biochem. 118:3643–3653. 2017. View Article : Google Scholar : PubMed/NCBI

30 

Gan L, Xu M, Zhang Y, Zhang X and Guo W: Focusing on long noncoding RNA dysregulation in gastric cancer. Tumour Biol. 36:129–141. 2015. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang XP, Song J, Liu GT, Wang JJ and Guo HF: Upregulation of gastric adenocarcinoma predictive long intergenic non‑coding RNA promotes progression and predicts poor prognosis in perihilar cholangiocarcinoma. Oncol Lett 16: 3964-3972, 2018.
APA
Wang, X., Song, J., Liu, G., Wang, J., & Guo, H. (2018). Upregulation of gastric adenocarcinoma predictive long intergenic non‑coding RNA promotes progression and predicts poor prognosis in perihilar cholangiocarcinoma. Oncology Letters, 16, 3964-3972. https://doi.org/10.3892/ol.2018.9137
MLA
Wang, X., Song, J., Liu, G., Wang, J., Guo, H."Upregulation of gastric adenocarcinoma predictive long intergenic non‑coding RNA promotes progression and predicts poor prognosis in perihilar cholangiocarcinoma". Oncology Letters 16.3 (2018): 3964-3972.
Chicago
Wang, X., Song, J., Liu, G., Wang, J., Guo, H."Upregulation of gastric adenocarcinoma predictive long intergenic non‑coding RNA promotes progression and predicts poor prognosis in perihilar cholangiocarcinoma". Oncology Letters 16, no. 3 (2018): 3964-3972. https://doi.org/10.3892/ol.2018.9137
Copy and paste a formatted citation
x
Spandidos Publications style
Wang XP, Song J, Liu GT, Wang JJ and Guo HF: Upregulation of gastric adenocarcinoma predictive long intergenic non‑coding RNA promotes progression and predicts poor prognosis in perihilar cholangiocarcinoma. Oncol Lett 16: 3964-3972, 2018.
APA
Wang, X., Song, J., Liu, G., Wang, J., & Guo, H. (2018). Upregulation of gastric adenocarcinoma predictive long intergenic non‑coding RNA promotes progression and predicts poor prognosis in perihilar cholangiocarcinoma. Oncology Letters, 16, 3964-3972. https://doi.org/10.3892/ol.2018.9137
MLA
Wang, X., Song, J., Liu, G., Wang, J., Guo, H."Upregulation of gastric adenocarcinoma predictive long intergenic non‑coding RNA promotes progression and predicts poor prognosis in perihilar cholangiocarcinoma". Oncology Letters 16.3 (2018): 3964-3972.
Chicago
Wang, X., Song, J., Liu, G., Wang, J., Guo, H."Upregulation of gastric adenocarcinoma predictive long intergenic non‑coding RNA promotes progression and predicts poor prognosis in perihilar cholangiocarcinoma". Oncology Letters 16, no. 3 (2018): 3964-3972. https://doi.org/10.3892/ol.2018.9137
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