Selective enrichment of CD133+/SOX2+ glioblastoma stem cells via adherent culture

  • Authors:
    • Ke Lv
    • Zhenyu Chen
    • Xiaoqing Zhang
    • Quanbin Zhang
    • Ling Liu
  • View Affiliations

  • Published online on: July 17, 2018     https://doi.org/10.3892/ol.2018.9154
  • Pages: 4567-4576
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Most of the brain tumors are malignant with an extremely poor survival rate. Recent progress in identifying cancer stem cells (CSCs) within the brain tumors is starting to revolutionize our understanding in the imitation and progression of tumors as well as relapse and the development of therapeutic strategies. Suspension spheroid body culture paradigm is a routine method in enriching CSCs. While, it was reported recently that CSCs within the brain tumor may also be enriched through adherent monolayer culture with optimized properties. In the present study, 18 surgically resected brain tumors were used for analyzing the feasibility of adherent enrichment of CSCs. The results indicated that 50% of glioblastomas were able to generate adherent CSCs, which were uniformly positive for Sox2, CD133, GFAP and Nestin. However, adherent culture paradigm failed to yield CSCs in secondary brain tumors, including neurocytomas, ependymomas, germ cell tumors or low‑grade astrocytomas, which is most likely due to a lack of CD133+/Sox2+ cells within the original biopsies. Therefore, it was concluded that the adherent culture paradigm may serve as a reliable method in enriching brain CSCs, but this method is more suitable for enriching CD133+/Sox2+ CSCs in glioblastomas.
View Figures
View References

Related Articles

Journal Cover

October-2018
Volume 16 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Lv K, Chen Z, Zhang X, Zhang Q and Liu L: Selective enrichment of CD133+/SOX2+ glioblastoma stem cells via adherent culture. Oncol Lett 16: 4567-4576, 2018
APA
Lv, K., Chen, Z., Zhang, X., Zhang, Q., & Liu, L. (2018). Selective enrichment of CD133+/SOX2+ glioblastoma stem cells via adherent culture. Oncology Letters, 16, 4567-4576. https://doi.org/10.3892/ol.2018.9154
MLA
Lv, K., Chen, Z., Zhang, X., Zhang, Q., Liu, L."Selective enrichment of CD133+/SOX2+ glioblastoma stem cells via adherent culture". Oncology Letters 16.4 (2018): 4567-4576.
Chicago
Lv, K., Chen, Z., Zhang, X., Zhang, Q., Liu, L."Selective enrichment of CD133+/SOX2+ glioblastoma stem cells via adherent culture". Oncology Letters 16, no. 4 (2018): 4567-4576. https://doi.org/10.3892/ol.2018.9154