Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
October-2018 Volume 16 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
October-2018 Volume 16 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Chaetominine induces cell cycle arrest in human leukemia K562 and colon cancer SW1116 cells

  • Authors:
    • Jingyun Yao
    • Jianbo Xiao
    • Xing Wei
    • Yanhua Lu
  • View Affiliations / Copyright

    Affiliations: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, P.R. China, Institute of Chinese Medical Sciences, State Key Laboratory of Quality Research in Chinese Medicine, University of Macau, Taipa, Macau 999078, P.R. China
  • Pages: 4671-4678
    |
    Published online on: July 17, 2018
       https://doi.org/10.3892/ol.2018.9161
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Chaetominine is a cytotoxic alkaloid that has been demonstrated to promote apoptotic cell death in human leukemia K562 cells. In the present study, chaetominine inhibited K562 (IC50 34 nM) and SW1116 (IC50 46 nM) cell growth. However, it remains unclear whether the inhibition of cell growth is associated with the cell cycle. To assess this potential relationship, the effect of chaetominine on the cell cycle of K562 and SW1116 cells was examined. Chaetominine treatment caused cell apoptosis and G1‑phase arrest in SW1116 cells. Conversely, K562 cells underwent S‑phase arrest according to flow cytometric analysis. The present study also aimed to elucidate the molecular mechanisms underpinning cell cycle regulation following the incubation of the associated cells with chaetominine. Western blot and reverse transcription‑quantitative polymerase chain reaction analyses suggested that chaetominine treatment facilitated the expression of p53, p21, checkpoint kinase 2 (Chk2) and phosphorylated ataxia telangiectasia mutated (p‑ATM) and caused a reduction in the mRNA levels of cyclin E and cyclin‑dependent kinases (CDKs) 2 and 4. These results suggest that chaetominine may be involved in the regulation of p53/p21 and ATM and Rad3‑related (ATM)/Chk2 signaling in SW1116 cells. Previous studies have demonstrated that these signaling pathways are responsible for G1‑phase arrest. Results of the present study demonstrated that the expression of p‑ATR and Chk1 were increased in K562 cells. Additionally, cdc25A levels were decreased, while protein and gene expression levels of cyclin A and CDK2 were repressed. These results elucidated the role of chaetominine in in the regulation of ATR/cdc25A/Chk1 expression in K562 cells. These proteins are thus important determinants in the initiation of S‑phase arrest. These data support the hypothesis that chaetominine is a potential anti‑cancer therapeutic agent that targets the cell cycle.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Hussain S, Fareed S, Ansari S and Khan S: Marine natural products: A lead for anti-cancer. Indian J Geo Mar Sci. 41:27–39. 2012.

2 

Imperatore C, Aiello A, D'Aniello F, Senese M and Menna M: Alkaloids from marine invertebrates as important leads for anticancer drugs discovery and development. Molecules. 19:20391–20423. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Lu YH, Zhu YX, Jiao RH, Tan RX, Yao LY and Hu WW: Method and medium for producing Fumigaclavine C by Aspergillus fumigatus Fermentation. China patent, CN 103849663 B. Filed January 10, 2014; issued May 4. 2016.

4 

Yao J, Jiao R, Liu C, Zhang Y, Yu W, Lu Y and Tan R: Assessment of the cytotoxic and apoptotic effects of chaetominine in a human leukemia cell line. Biomol Ther (Seoul). 24:147–155. 2016. View Article : Google Scholar : PubMed/NCBI

5 

Mahata S, Bharti AC, Shukla S, Tyagi A, Husain SA and Das BC: Berberine modulates AP-1 activity to suppress HPV transcription and downstream signaling to induce growth arrest and apoptosis in cervical cancer cells. Mol Cancer. 10:392011. View Article : Google Scholar : PubMed/NCBI

6 

Li S, Lei Y, Jia Y, Li N, Wink M and Ma Y: Piperine, a piperidine alkaloid from Piper nigrum re-sensitizes P-gp, MRP1 and BCRP dependent multidrug resistant cancer cells. Phytomedicine. 19:83–87. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Kastan MB and Bartek J: Cell-cycle checkpoints and cancer. Nature. 432:316–323. 2004. View Article : Google Scholar : PubMed/NCBI

8 

Kerzendorfer C and O'Driscoll M: Human DNA damage response and repair deficiency syndromes: Linking genomic instability and cell cycle checkpoint proficiency. DNA Repair (Amst). 8:1139–1152. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Sánchez-Martínez C, Gelbert LM, Lallena MJ and de Dios A: Cyclin dependent kinase (CDK) inhibitors as anticancer drugs. Bioorg Med Chem Lett. 25:3420–3435. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Poi MJ, Knobloch TJ, Sears MT, Uhrig LK, Warner BM, Weghorst CM and Li J: Coordinated expression of cyclin-dependent kinase-4 and its regulators in human oral tumors. Anticancer Res. 34:3285–3292. 2014.PubMed/NCBI

11 

Jiao RH, Xu S, Liu JY, Ge HM, Ding H, Xu C, Zhu HL and Tan RX: Chaetominine, a cytotoxic alkaloid produced by endophytic Chaetomium sp. IFB-E015. Org Lett. 8:5709–5712. 2006. View Article : Google Scholar : PubMed/NCBI

12 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

13 

Smith J, Tho LM, Xu N and Gillespie DA: The ATM-Chk2 and ATR-Chk1 pathways in DNA damage signaling and cancer. Adv Cancer Res. 108:73–112. 2010. View Article : Google Scholar : PubMed/NCBI

14 

Yam CH, Fung TK and Poon RY: Cyclin A in cell cycle control and cancer. Cell Mol Life Sci. 59:1317–1326. 2002. View Article : Google Scholar : PubMed/NCBI

15 

Berthet C, Raj K, Saudan P and Beard P: How adeno-associated virus Rep78 protein arrests cells completely in S phase. Proc Natl Acad Sci USA. 102:13634–13639. 2005. View Article : Google Scholar : PubMed/NCBI

16 

Kshirsagar UA: Recent developments in the chemistry of quinazolinone alkaloids. Org Biomol Chem. 13:9336–9352. 2015. View Article : Google Scholar : PubMed/NCBI

17 

Wahl GM and Carr AM: The evolution of diverse biological responses to DNA damage: Insights from yeast and p53. Nat Cell Biol. 3:E277–E286. 2001. View Article : Google Scholar : PubMed/NCBI

18 

Zhang J, Ghio AJ, Gao M, Wei K, Rosen GD and Upadhyay D: Ambient particulate matter induces alveolar epithelial cell cycle arrest: Role of G1 cyclins. FEBS Lett. 581:5315–5320. 2007. View Article : Google Scholar : PubMed/NCBI

19 

Bartek J, Lukas C and Lukas J: Checking on DNA damage in S phase. Nat Rev Mol Cell Biol. 5:792–804. 2004. View Article : Google Scholar : PubMed/NCBI

20 

Zuco V, Benedetti V and Zunino F: ATM- and ATR-mediated response to DNA damage induced by a novel camptothecin, ST1968. Cancer Lett. 292:186–196. 2010. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Yao J, Xiao J, Wei X and Lu Y: Chaetominine induces cell cycle arrest in human leukemia K562 and colon cancer SW1116 cells. Oncol Lett 16: 4671-4678, 2018.
APA
Yao, J., Xiao, J., Wei, X., & Lu, Y. (2018). Chaetominine induces cell cycle arrest in human leukemia K562 and colon cancer SW1116 cells. Oncology Letters, 16, 4671-4678. https://doi.org/10.3892/ol.2018.9161
MLA
Yao, J., Xiao, J., Wei, X., Lu, Y."Chaetominine induces cell cycle arrest in human leukemia K562 and colon cancer SW1116 cells". Oncology Letters 16.4 (2018): 4671-4678.
Chicago
Yao, J., Xiao, J., Wei, X., Lu, Y."Chaetominine induces cell cycle arrest in human leukemia K562 and colon cancer SW1116 cells". Oncology Letters 16, no. 4 (2018): 4671-4678. https://doi.org/10.3892/ol.2018.9161
Copy and paste a formatted citation
x
Spandidos Publications style
Yao J, Xiao J, Wei X and Lu Y: Chaetominine induces cell cycle arrest in human leukemia K562 and colon cancer SW1116 cells. Oncol Lett 16: 4671-4678, 2018.
APA
Yao, J., Xiao, J., Wei, X., & Lu, Y. (2018). Chaetominine induces cell cycle arrest in human leukemia K562 and colon cancer SW1116 cells. Oncology Letters, 16, 4671-4678. https://doi.org/10.3892/ol.2018.9161
MLA
Yao, J., Xiao, J., Wei, X., Lu, Y."Chaetominine induces cell cycle arrest in human leukemia K562 and colon cancer SW1116 cells". Oncology Letters 16.4 (2018): 4671-4678.
Chicago
Yao, J., Xiao, J., Wei, X., Lu, Y."Chaetominine induces cell cycle arrest in human leukemia K562 and colon cancer SW1116 cells". Oncology Letters 16, no. 4 (2018): 4671-4678. https://doi.org/10.3892/ol.2018.9161
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team