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Article

Evaluation of the mRNA expression levels of integrins α3, α5, β1 and β6 as tumor biomarkers of oral squamous cell carcinoma

  • Authors:
    • Hsueh‑Wei Chang
    • Ching‑Yu Yen
    • Chung‑Ho Chen
    • Jun‑Hsu Tsai
    • Jen‑Yang Tang
    • Yung‑Ting Chang
    • Yu‑Hsun Kao
    • Yen‑Yun Wang
    • Shyng‑Shiou F. Yuan
    • Sheng‑Yang Lee
  • View Affiliations / Copyright

    Affiliations: Department of Biomedical Sciences and Environmental Biology, Kaohsiung Medical University, Kaohsiung 80708, Taiwan, R.O.C., Department of Oral and Maxillofacial Surgery, Chi‑Mei Medical Center, Tainan 71004, Taiwan, R.O.C., Department of Dentistry, Kaohsiung Municipal Hsiao Kang Hospital, Kaohsiung 81267, Taiwan, R.O.C., Department of Oral and Maxillofacial Surgery, Kaohsiung Medical University Hospital, Kaohsiung 80708, Taiwan, R.O.C., Department of Radiation Oncology, Faculty of Medicine, College of Medicine, Kaohsiung Medical University Hospital, Kaohsiung 80708, Taiwan, R.O.C., Doctoral Degree Program in Marine Biotechnology, National Sun Yat‑sen University, Kaohsiung 80424, Taiwan, R.O.C., School of Dentistry, College of Dental Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan, R.O.C., Cancer Center, Kaohsiung Medical University Hospital, Kaohsiung 80708, Taiwan, R.O.C., School of Dentistry, Taipei Medical University, Taipei 11031, Taiwan, R.O.C.
  • Pages: 4773-4781
    |
    Published online on: July 18, 2018
       https://doi.org/10.3892/ol.2018.9168
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Abstract

Integrin signaling may modulate several different functions involved in cell migration, invasion, proliferation and motility, and is a potential candidate biomarker for oral cancer. In the present study, a total of four integrin genes were evaluated as potential biomarkers of oral squamous cell carcinoma (OSCC). Gene expression was determined using the reverse transcription‑quantitative polymerase chain reaction in 55 OSCC and 55 matched normal oral tissues. The performance of individual and combined biomarkers was analyzed by receiver operating characteristic (ROC) analysis based on the relative mRNA expression (OSCC vs. matched oral tissue from the tumor‑free margin), which was calculated using the ΔΔCq value (ΔCq of OSCC‑ΔCq of oral tissue from the tumor‑free margin of the same patient). In the individual ROC analysis, the areas under the ROC curve (AUCs) of relative mRNA expression (ΔΔCq) of integrin subunit α3 (ITGA3), integrin subunit α5 (ITGA5), integrin subunit β1 (ITGB1) and integrin subunit β6 (ITGB6) in all tumor locations were 0.724, 0.698, 0.640 and 0.657, respectively. For locations 2 (tongue/mouth part) and 3 (edentulous ridge), their individual AUC values were 0.840, 0.765, 0.725 and 0.763, respectively. In the cumulative ROC analysis, ITGA3, ITGA5 and ITGB1 genes exhibited the highest combined AUC values (0.809 and 0.871 for all locations and locations 2 and 3 combined, respectively) compared with other biomarker combinations. In conclusion, the results of the present study identified that higher mRNA expressions of ITGA3, ITGA5, ITGB1 and ITGB6 genes are suitable for OSCC diagnosis biomarkers. Cumulative ROC analysis indicated an improved overall performance compared with the best individual integrin biomarker of OSCC.
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1 

Shah JP and Gil Z: Current concepts in management of oral cancer-surgery. Oral Oncol. 45:394–401. 2009. View Article : Google Scholar : PubMed/NCBI

2 

Dumache R, Rogobete AF, Andreescu N and Puiu M: Genetic and epigenetic biomarkers of molecular alterations in oral carcinogenesis. Clin Lab. 61:1373–1381. 2015. View Article : Google Scholar : PubMed/NCBI

3 

Nagata M, Noman AA, Suzuki K, Kurita H, Ohnishi M, Ohyama T, Kitamura N, Kobayashi T, Uematsu K, Takahashi K, et al: ITGA3 and ITGB4 expression biomarkers estimate the risks of locoregional and hematogenous dissemination of oral squamous cell carcinoma. BMC Cancer. 13:4102013. View Article : Google Scholar : PubMed/NCBI

4 

Elashoff D, Zhou H, Reiss J, Wang J, Xiao H, Henson B, Hu S, Arellano M, Sinha U, Le A, et al: Prevalidation of salivary biomarkers for oral cancer detection. Cancer Epidemiol Biomarkers Prev. 21:664–672. 2012. View Article : Google Scholar : PubMed/NCBI

5 

Yen CY, Chen CH, Chang CH, Tseng HF, Liu SY, Chuang LY, Wen CH and Chang HW: Matrix metalloproteinases (MMP) 1 and MMP10 but not MMP12 are potential oral cancer markers. Biomarkers. 14:244–249. 2009. View Article : Google Scholar : PubMed/NCBI

6 

Yen CY, Huang CY, Hou MF, Yang YH, Chang CH, Huang HW, Chen CH and Chang HW: Evaluating the performance of fibronectin 1 (FN1), integrin α4β1 (ITGA4), syndecan-2 (SDC2) and glycoprotein CD44 as the potential biomarkers of oral squamous cell carcinoma (OSCC). Biomarkers. 18:63–72. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Wang S, Sun M, Gu C, Wang X, Chen D, Zhao E, Jiao X and Zheng J: Expression of CD163, interleukin-10, and interferon-gamma in oral squamous cell carcinoma: Mutual relationships and prognostic implications. Eur J Oral Sci. 122:202–209. 2014. View Article : Google Scholar : PubMed/NCBI

8 

Barczyk M, Carracedo S and Gullberg D: Integrins. Cell Tissue Res. 339:269–280. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Hood JD and Cheresh DA: Role of integrins in cell invasion and migration. Nat Rev Cancer. 2:91–100. 2002. View Article : Google Scholar : PubMed/NCBI

10 

Desgrosellier JS and Cheresh DA: Integrins in cancer: Biological implications and therapeutic opportunities. Nat Rev Cancer. 10:9–22. 2010. View Article : Google Scholar : PubMed/NCBI

11 

Malinin NL, Pluskota E and Byzova TV: Integrin signaling in vascular function. Curr Opin Hematol. 19:206–211. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Mizejewski GJ: Role of integrins in cancer: Survey of expression patterns. Proc Soc Exp Biol Med. 222:124–138. 1999. View Article : Google Scholar : PubMed/NCBI

13 

Hwang R and Varner J: The role of integrins in tumor angiogenesis. Hematol Oncol Clin North Am. 18:991–1006, vii. 2004. View Article : Google Scholar : PubMed/NCBI

14 

Hynes RO: Integrins: Versatility, modulation, and signaling in cell adhesion. Cell. 69:11–25. 1992. View Article : Google Scholar : PubMed/NCBI

15 

Yamaguchi M, Ebihara N, Shima N, Kimoto M, Funaki T, Yokoo S, Murakami A and Yamagami S: Adhesion, migration, and proliferation of cultured human corneal endothelial cells by laminin-5. Invest Ophthalmol Vis Sci. 52:679–684. 2011. View Article : Google Scholar : PubMed/NCBI

16 

DiPersio CM, Hodivala-Dilke KM, Jaenisch R, Kreidberg JA and Hynes RO: alpha3beta1 Integrin is required for normal development of the epidermal basement membrane. J Cell Biol. 137:729–742. 1997. View Article : Google Scholar : PubMed/NCBI

17 

Kreidberg JA: Functions of alpha3beta1 integrin. Curr Opin Cell Biol. 12:548–553. 2000. View Article : Google Scholar : PubMed/NCBI

18 

Tsuji T: Physiological and pathological roles of alpha3beta1 integrin. J Membr Biol. 200:115–132. 2004. View Article : Google Scholar : PubMed/NCBI

19 

Takenaka K, Shibuya M, Takeda Y, Hibino S, Gemma A, Ono Y and Kudoh S: Altered expression and function of beta1 integrins in a highly metastatic human lung adenocarcinoma cell line. Int J Oncol. 17:1187–1194. 2000.PubMed/NCBI

20 

Coopman PJ, Thomas DM, Gehlsen KR and Mueller SC: Integrin alpha 3 beta 1 participates in the phagocytosis of extracellular matrix molecules by human breast cancer cells. Mol Biol Cell. 7:1789–1804. 1996. View Article : Google Scholar : PubMed/NCBI

21 

Adachi M, Taki T, Higashiyama M, Kohno N, Inufusa H and Miyake M: Significance of integrin alpha5 gene expression as a prognostic factor in node-negative non-small cell lung cancer. Clin Cancer Res. 6:96–101. 2000.PubMed/NCBI

22 

Ryu MH, Park HM, Chung J, Lee CH and Park HR: Hypoxia-inducible factor-1alpha mediates oral squamous cell carcinoma invasion via upregulation of alpha5 integrin and fibronectin. Biochem Biophys Res Commun. 393:11–15. 2010. View Article : Google Scholar : PubMed/NCBI

23 

Bandyopadhyay A and Raghavan S: Defining the role of integrin alphavbeta6 in cancer. Curr Drug Targets. 10:645–652. 2009. View Article : Google Scholar : PubMed/NCBI

24 

Ramos DM, Dang D and Sadler S: The role of the integrin alpha v beta6 in regulating the epithelial to mesenchymal transition in oral cancer. Anticancer Res. 29:125–130. 2009.PubMed/NCBI

25 

Ramos DM, But M, Regezi J, Schmidt BL, Atakilit A, Dang D, Ellis D, Jordan R and Li X: Expression of integrin beta 6 enhances invasive behavior in oral squamous cell carcinoma. Matrix Biol. 21:297–307. 2002. View Article : Google Scholar : PubMed/NCBI

26 

Greene FL, Page DL, Fleming ID, Fritz AG, Balch CM, Haller DG and Morrow M: AJCC cancer staging manual. Springer-Verlag; New York: 2002, View Article : Google Scholar

27 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

28 

Chang HW, Cheng CA, Gu DL, Chang CC, Su SH, Wen CH, Chou YC, Chou TC, Yao CT, Tsai CL and Cheng CC: High-throughput avian molecular sexing by SYBR green-based real-time PCR combined with melting curve analysis. BMC Biotechnol. 8:122008. View Article : Google Scholar : PubMed/NCBI

29 

Shih IeM, Salani R, Fiegl M, Wang TL, Soosaipillai A, Marth C, Müller-Holzner E, Gastl G, Zhang Z and Diamandis EP: Ovarian cancer specific kallikrein profile in effusions. Gynecol Oncol. 105:501–507. 2007. View Article : Google Scholar : PubMed/NCBI

30 

Wong HS and Chang WC: Correlation of clinical features and genetic profiles of stromal interaction molecule 1 (STIM1) in colorectal cancers. Oncotarget. 6:42169–42182. 2015. View Article : Google Scholar : PubMed/NCBI

31 

Castilho LS, Cotta FV, Bueno AC, Moreira AN, Ferreira EF and Magalhães CS: Validation of DIAGNOdent laser fluorescence and the international caries detection and assessment system (ICDAS) in diagnosis of occlusal caries in permanent teeth: An in vivo study. Eur J Oral Sci. 124:188–194. 2016. View Article : Google Scholar : PubMed/NCBI

32 

Zhang B, Zhang Z, Zhang X, Gao X, Kernstine KH and Zhong L: Serological antibodies against LY6K as a diagnostic biomarker in esophageal squamous cell carcinoma. Biomarkers. 17:372–378. 2012. View Article : Google Scholar : PubMed/NCBI

33 

Cai C, Shi R, Gao Y, Zeng J, Wei M, Wang H, Zheng W and Ma W: Reduced expression of sushi domain containing 2 is associated with progression of non-small cell lung cancer. Oncol Lett. 10:3619–3624. 2015. View Article : Google Scholar : PubMed/NCBI

34 

Sun L, Xu S, Liang L, Zhao L and Zhang L: Analysis of ROC: The value of HPV16 E6 protein in the diagnosis of early stage cervical carcinoma and precancerous lesions. Oncol Lett. 12:1769–1772. 2016. View Article : Google Scholar : PubMed/NCBI

35 

Kurozumi A, Goto Y, Matsushita R, Fukumoto I, Kato M, Nishikawa R, Sakamoto S, Enokida H, Nakagawa M, Ichikawa T and Seki N: Tumor-suppressive microRNA-223 inhibits cancer cell migration and invasion by targeting ITGA3/ITGB1 signaling in prostate cancer. Cancer Sci. 107:84–94. 2016. View Article : Google Scholar : PubMed/NCBI

36 

Zheng W, Jiang C and Li R: Integrin and gene network analysis reveals that ITGA5 and ITGB1 are prognostic in non-small-cell lung cancer. Onco Targets Ther. 9:2317–2327. 2016. View Article : Google Scholar : PubMed/NCBI

37 

Desai K, Nair MG, Prabhu JS, Vinod A, Korlimarla A, Rajarajan S, Aiyappa R, Kaluve RS, Alexander A, Hari PS, et al: High expression of integrin β6 in association with the Rho-Rac pathway identifies a poor prognostic subgroup within HER2 amplified breast cancers. Cancer Med. 5:2000–2011. 2016. View Article : Google Scholar : PubMed/NCBI

38 

He M, Zhao Y, Yi H, Sun H, Liu X and Ma S: The combination of TP53INP1, TP53INP2 and AXIN2: Potential biomarkers in papillary thyroid carcinoma. Endocrine. 48:712–717. 2015. View Article : Google Scholar : PubMed/NCBI

39 

Rozalski R, Gackowski D, Siomek-Gorecka A, Starczak M, Modrzejewska M, Banaszkiewicz Z and Olinski R: Urinary 5-hydroxymethyluracil and 8-oxo-7,8-dihydroguanine as potential biomarkers in patients with colorectal cancer. Biomarkers. 20:287–291. 2015. View Article : Google Scholar : PubMed/NCBI

40 

El-mezayen HA, Metwally FM and Darwish H: A novel discriminant score based on tumor-associated trypsin inhibitor for accurate diagnosis of metastasis in patients with breast cancer. Tumour Biol. 35:2759–2767. 2014. View Article : Google Scholar : PubMed/NCBI

41 

Yin MZ, Tan S, Li X, Hou Y, Cao G, Li K, Kou J and Lou G: Identification of phosphatidylcholine and lysophosphatidylcholine as novel biomarkers for cervical cancers in a prospective cohort study. Tumour Biol. 37:5485–5492. 2016. View Article : Google Scholar : PubMed/NCBI

42 

Arcolia V, Journe F, Renaud F, Leteurtre E, Gabius HJ, Remmelink M and Saussez S: Combination of galectin-3, CK19 and HBME-1 immunostaining improves the diagnosis of thyroid cancer. Oncol Lett. 14:4183–4189. 2017. View Article : Google Scholar : PubMed/NCBI

43 

Michailidou E, Tzimagiorgis G, Chatzopoulou F, Vahtsevanos K, Antoniadis K, Kouidou S, Markopoulos A and Antoniades D: Salivary mRNA markers having the potential to detect oral squamous cell carcinoma segregated from oral leukoplakia with dysplasia. Cancer Epidemiol. 43:112–118. 2016. View Article : Google Scholar : PubMed/NCBI

44 

Bu J, Bu X, Liu B, Chen F and Chen P: Increased expression of tissue/salivary transgelin mRNA predicts poor prognosis in patients with oral squamous cell carcinoma (OSCC). Med Sci Monit. 21:2275–2281. 2015. View Article : Google Scholar : PubMed/NCBI

45 

Grady LJ, Campbell WP and North AB: A comparison of several procedures for reducing RNase contamination in preparations of DNase and a particularly successful combination of methods. Anal Biochem. 101:118–122. 1980. View Article : Google Scholar : PubMed/NCBI

46 

Febbraio F, Andolfo A, Tanfani F, Briante R, Gentile F, Formisano S, Vaccaro C, Scirè A, Bertoli E, Pucci P and Nucci R: Thermal stability and aggregation of sulfolobus solfataricus beta-glycosidase are dependent upon the N-epsilon-methylation of specific lysyl residues: Critical role of in vivo post-translational modifications. J Biol Chem. 279:10185–10194. 2004. View Article : Google Scholar : PubMed/NCBI

47 

Ko YC, Huang YL, Lee CH, Chen MJ, Lin LM and Tsai CC: Betel quid chewing, cigarette smoking and alcohol consumption related to oral cancer in Taiwan. J Oral Pathol Med. 24:450–453. 1995. View Article : Google Scholar : PubMed/NCBI

48 

Petti S, Mohd M and Scully C: Revisiting the association between alcohol drinking and oral cancer in nonsmoking and betel quid non-chewing individuals. Cancer Epidemiol. 36:e1–e6. 2012. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Chang HW, Yen CY, Chen CH, Tsai JH, Tang JY, Chang YT, Kao YH, Wang YY, Yuan SSF, Lee SY, Lee SY, et al: Evaluation of the mRNA expression levels of integrins α3, α5, β1 and β6 as tumor biomarkers of oral squamous cell carcinoma. Oncol Lett 16: 4773-4781, 2018.
APA
Chang, H., Yen, C., Chen, C., Tsai, J., Tang, J., Chang, Y. ... Lee, S. (2018). Evaluation of the mRNA expression levels of integrins α3, α5, β1 and β6 as tumor biomarkers of oral squamous cell carcinoma. Oncology Letters, 16, 4773-4781. https://doi.org/10.3892/ol.2018.9168
MLA
Chang, H., Yen, C., Chen, C., Tsai, J., Tang, J., Chang, Y., Kao, Y., Wang, Y., Yuan, S. F., Lee, S."Evaluation of the mRNA expression levels of integrins α3, α5, β1 and β6 as tumor biomarkers of oral squamous cell carcinoma". Oncology Letters 16.4 (2018): 4773-4781.
Chicago
Chang, H., Yen, C., Chen, C., Tsai, J., Tang, J., Chang, Y., Kao, Y., Wang, Y., Yuan, S. F., Lee, S."Evaluation of the mRNA expression levels of integrins α3, α5, β1 and β6 as tumor biomarkers of oral squamous cell carcinoma". Oncology Letters 16, no. 4 (2018): 4773-4781. https://doi.org/10.3892/ol.2018.9168
Copy and paste a formatted citation
x
Spandidos Publications style
Chang HW, Yen CY, Chen CH, Tsai JH, Tang JY, Chang YT, Kao YH, Wang YY, Yuan SSF, Lee SY, Lee SY, et al: Evaluation of the mRNA expression levels of integrins α3, α5, β1 and β6 as tumor biomarkers of oral squamous cell carcinoma. Oncol Lett 16: 4773-4781, 2018.
APA
Chang, H., Yen, C., Chen, C., Tsai, J., Tang, J., Chang, Y. ... Lee, S. (2018). Evaluation of the mRNA expression levels of integrins α3, α5, β1 and β6 as tumor biomarkers of oral squamous cell carcinoma. Oncology Letters, 16, 4773-4781. https://doi.org/10.3892/ol.2018.9168
MLA
Chang, H., Yen, C., Chen, C., Tsai, J., Tang, J., Chang, Y., Kao, Y., Wang, Y., Yuan, S. F., Lee, S."Evaluation of the mRNA expression levels of integrins α3, α5, β1 and β6 as tumor biomarkers of oral squamous cell carcinoma". Oncology Letters 16.4 (2018): 4773-4781.
Chicago
Chang, H., Yen, C., Chen, C., Tsai, J., Tang, J., Chang, Y., Kao, Y., Wang, Y., Yuan, S. F., Lee, S."Evaluation of the mRNA expression levels of integrins α3, α5, β1 and β6 as tumor biomarkers of oral squamous cell carcinoma". Oncology Letters 16, no. 4 (2018): 4773-4781. https://doi.org/10.3892/ol.2018.9168
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