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Article Open Access

miR‑204 enhances p27 mRNA stability by targeting Brd4 in head and neck squamous cell carcinoma

  • Authors:
    • Caihua Wang
    • Yonghong Zhang
    • Dayong Zhou
    • Guanjie Cao
    • Yungang Wu
  • View Affiliations / Copyright

    Affiliations: Department of Ear, Nose and Throat, The Affiliated Hospital of Jining Medical College, Jining, Shandong 272000, P.R. China, Department of Orthopedics, Hengkang Hospital, Jining, Shandong 272000, P.R. China, Department of Imaging, The Affiliated Hospital of Jining Medical College, Jining, Shandong 272000, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4179-4184
    |
    Published online on: July 19, 2018
       https://doi.org/10.3892/ol.2018.9180
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Abstract

The present study aimed to explore the function of microRNA (miR)‑204 in modulating cyclin‑dependent kinase inhibitor 1B (p27) mRNA stability in head and neck squamous cell carcinoma (HNSCC). Briefly, reverse transcription quantitative polymerase chain reaction and western blot analysis were used to detect miR‑204 and Brd4 level. Cell viability, cell cycle and cell apoptosis were used to investigate the effects of miR‑204. Additional luciferase reporter and mRNA stability assays were used to explore the mechanisms contributing to miR‑204 effects. Here, miR‑204 was downregulated in HNSCC tissues compared with the adjacent normal tissues. The expression levels of miR‑204 and bromodomain‑containing protein 4 (Brd4) were negatively associated in HNSCC tissues. Ectopic expression of miR‑204 inhibited HNSCC cell proliferation, promoted cell cycle arrest at the G1/S phase and promoted cell apoptosis compared with control cells. Additionally, upregulation of miR‑204 expression levels enhanced p27 mRNA stability. Notably, Brd4 was identified as a target of miR‑204, and the co‑expression of Brd4 with miR‑204 mimics attenuated the inhibitory effects of miR‑204 on cell proliferation and enhanced p27 mRNA stability compared with control cells. Thus, it was concluded that miR‑204 functions as a tumor suppressor by enhancing p27 mRNA stability through targeting Brd4 in HNSCC.
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Copy and paste a formatted citation
Spandidos Publications style
Wang C, Zhang Y, Zhou D, Cao G and Wu Y: miR‑204 enhances p27 mRNA stability by targeting Brd4 in head and neck squamous cell carcinoma. Oncol Lett 16: 4179-4184, 2018.
APA
Wang, C., Zhang, Y., Zhou, D., Cao, G., & Wu, Y. (2018). miR‑204 enhances p27 mRNA stability by targeting Brd4 in head and neck squamous cell carcinoma. Oncology Letters, 16, 4179-4184. https://doi.org/10.3892/ol.2018.9180
MLA
Wang, C., Zhang, Y., Zhou, D., Cao, G., Wu, Y."miR‑204 enhances p27 mRNA stability by targeting Brd4 in head and neck squamous cell carcinoma". Oncology Letters 16.4 (2018): 4179-4184.
Chicago
Wang, C., Zhang, Y., Zhou, D., Cao, G., Wu, Y."miR‑204 enhances p27 mRNA stability by targeting Brd4 in head and neck squamous cell carcinoma". Oncology Letters 16, no. 4 (2018): 4179-4184. https://doi.org/10.3892/ol.2018.9180
Copy and paste a formatted citation
x
Spandidos Publications style
Wang C, Zhang Y, Zhou D, Cao G and Wu Y: miR‑204 enhances p27 mRNA stability by targeting Brd4 in head and neck squamous cell carcinoma. Oncol Lett 16: 4179-4184, 2018.
APA
Wang, C., Zhang, Y., Zhou, D., Cao, G., & Wu, Y. (2018). miR‑204 enhances p27 mRNA stability by targeting Brd4 in head and neck squamous cell carcinoma. Oncology Letters, 16, 4179-4184. https://doi.org/10.3892/ol.2018.9180
MLA
Wang, C., Zhang, Y., Zhou, D., Cao, G., Wu, Y."miR‑204 enhances p27 mRNA stability by targeting Brd4 in head and neck squamous cell carcinoma". Oncology Letters 16.4 (2018): 4179-4184.
Chicago
Wang, C., Zhang, Y., Zhou, D., Cao, G., Wu, Y."miR‑204 enhances p27 mRNA stability by targeting Brd4 in head and neck squamous cell carcinoma". Oncology Letters 16, no. 4 (2018): 4179-4184. https://doi.org/10.3892/ol.2018.9180
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