Open Access

β-Bourbonene attenuates proliferation and induces apoptosis of prostate cancer cells

  • Authors:
    • Zhong Wang
    • Feng Liu
    • Jian-Jun Yu
    • Ji-Zhong Jin
  • View Affiliations

  • Published online on: July 20, 2018     https://doi.org/10.3892/ol.2018.9183
  • Pages: 4519-4525
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Abstract

Sesquiterpenes have antitumor, anti-inflammation, and anti-fungal effects. β-bourbonene is a kind of sesquiterpene, but its pharmacological effect has not been studied. The present study was conducted in order to investigate the potential anticancer effects of β-bourbonene on human prostate cancer PC-3M cells. PC-3M cells were incubated with 0, 25, 50, 100 µg/ml of β-bourbonene. Cell Counting Kit-8 (CCK-8) detection showed that compared with the control group, β-bourbonene inhibited the growth of PC-3M cells in a dose-dependent manner. G0/G1 phase arrest was observed by β-bourbonene by using flow cytometry. TUNEL staining and Annexin V/PI dual-staining method revealed that apoptosis was found in cells with β-bourbonene treatment, and the quantity of apoptotic cells was increased with the elevation in concentration. The mRNA and protein expression levels of Fas and FasL in the drug-treatment group were significantly elevated. Furthermore, the western blot assay also indicated that with an increase in the concentration of β-bourbonene, the protein expression of Bax in the drug-treatment group was significantly elevated, while a decrease was identified in the protein expression of Bcl-2. Taken together, β-bourbonene can inhibit the proliferation and simultaneously, induce apoptosis and G0/G1 arrest of prostate cancer PC-3M cells, which may be realized by upregulation of mRNA expression of Fas and FasL, increase of Bax protein expression and decrease of Bcl-2 protein expression.
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October-2018
Volume 16 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Wang Z, Liu F, Yu J and Jin J: β-Bourbonene attenuates proliferation and induces apoptosis of prostate cancer cells. Oncol Lett 16: 4519-4525, 2018
APA
Wang, Z., Liu, F., Yu, J., & Jin, J. (2018). β-Bourbonene attenuates proliferation and induces apoptosis of prostate cancer cells. Oncology Letters, 16, 4519-4525. https://doi.org/10.3892/ol.2018.9183
MLA
Wang, Z., Liu, F., Yu, J., Jin, J."β-Bourbonene attenuates proliferation and induces apoptosis of prostate cancer cells". Oncology Letters 16.4 (2018): 4519-4525.
Chicago
Wang, Z., Liu, F., Yu, J., Jin, J."β-Bourbonene attenuates proliferation and induces apoptosis of prostate cancer cells". Oncology Letters 16, no. 4 (2018): 4519-4525. https://doi.org/10.3892/ol.2018.9183