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Article Open Access

H19 contributes to poor clinical features in NSCLC patients and leads to enhanced invasion in A549 cells through regulating miRNA‑203‑mediated epithelial‑mesenchymal transition

  • Authors:
    • Xiao‑Jun Ge
    • Li‑Mei Zheng
    • Zhong‑Xin Feng
    • Mei‑Yong Li
    • Lan Liu
    • Yu‑Jie Zhao
    • Jun‑Yao Jiang
  • View Affiliations / Copyright

    Affiliations: Department of Laboratory Medicine, Affiliated Hospital of Zunyi Medical College, Zunyi, Guizhou 563003, P.R. China
    Copyright: © Ge et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4480-4488
    |
    Published online on: July 23, 2018
       https://doi.org/10.3892/ol.2018.9187
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Abstract

Recent studies have demonstrated that the overexpression of H19 may contribute towards development of tumorigenesis in various types of cancer. To investigate the role of H19 in the development of non‑small cell lung cancer (NSCLC), 76 NSCLC tissues samples and their adjacent normal tissue samples were collected. Expression level of H19, and its association with clinicopathological features and overall survival was analyzed. It was found that compared with normal adjacent tissues, H19 expression was elevated in NSCLC tissues along with a decreased miR‑203 expression level. It was also found that patients who were in advanced clinical stages had a higher H19 and a lower miR‑203 expression compared to normal tissues. The overall survival time of patients with higher H19 expression was shorter compared with the lower H19 expression group. Upregulation of A549 enhanced cell proliferation and promoted invasion. Overexpression of H19 stimulated the epithelial‑mesenchymal transition (EMT) process in lung cancer cells and demonstrated typical morphological characteristics of EMT. The level of mesenchymal marker protein, such as Vimentin and SNAI1 increased; while CDH1 protein level decreased. Also, H19 negatively regulated miR‑203. Inhibition of H19 attenuated miR‑203 induced EMT process. Upregulation of H19 contributes to poor clinical features in patients with NSCLC, induces occurrence of EMT, promotes proliferation and stimulates cell invasion in NSCLC cell line through regulating miRNA‑203 mediated EMT.
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Copy and paste a formatted citation
Spandidos Publications style
Ge XJ, Zheng LM, Feng ZX, Li MY, Liu L, Zhao YJ and Jiang JY: H19 contributes to poor clinical features in NSCLC patients and leads to enhanced invasion in A549 cells through regulating miRNA‑203‑mediated epithelial‑mesenchymal transition. Oncol Lett 16: 4480-4488, 2018.
APA
Ge, X., Zheng, L., Feng, Z., Li, M., Liu, L., Zhao, Y., & Jiang, J. (2018). H19 contributes to poor clinical features in NSCLC patients and leads to enhanced invasion in A549 cells through regulating miRNA‑203‑mediated epithelial‑mesenchymal transition. Oncology Letters, 16, 4480-4488. https://doi.org/10.3892/ol.2018.9187
MLA
Ge, X., Zheng, L., Feng, Z., Li, M., Liu, L., Zhao, Y., Jiang, J."H19 contributes to poor clinical features in NSCLC patients and leads to enhanced invasion in A549 cells through regulating miRNA‑203‑mediated epithelial‑mesenchymal transition". Oncology Letters 16.4 (2018): 4480-4488.
Chicago
Ge, X., Zheng, L., Feng, Z., Li, M., Liu, L., Zhao, Y., Jiang, J."H19 contributes to poor clinical features in NSCLC patients and leads to enhanced invasion in A549 cells through regulating miRNA‑203‑mediated epithelial‑mesenchymal transition". Oncology Letters 16, no. 4 (2018): 4480-4488. https://doi.org/10.3892/ol.2018.9187
Copy and paste a formatted citation
x
Spandidos Publications style
Ge XJ, Zheng LM, Feng ZX, Li MY, Liu L, Zhao YJ and Jiang JY: H19 contributes to poor clinical features in NSCLC patients and leads to enhanced invasion in A549 cells through regulating miRNA‑203‑mediated epithelial‑mesenchymal transition. Oncol Lett 16: 4480-4488, 2018.
APA
Ge, X., Zheng, L., Feng, Z., Li, M., Liu, L., Zhao, Y., & Jiang, J. (2018). H19 contributes to poor clinical features in NSCLC patients and leads to enhanced invasion in A549 cells through regulating miRNA‑203‑mediated epithelial‑mesenchymal transition. Oncology Letters, 16, 4480-4488. https://doi.org/10.3892/ol.2018.9187
MLA
Ge, X., Zheng, L., Feng, Z., Li, M., Liu, L., Zhao, Y., Jiang, J."H19 contributes to poor clinical features in NSCLC patients and leads to enhanced invasion in A549 cells through regulating miRNA‑203‑mediated epithelial‑mesenchymal transition". Oncology Letters 16.4 (2018): 4480-4488.
Chicago
Ge, X., Zheng, L., Feng, Z., Li, M., Liu, L., Zhao, Y., Jiang, J."H19 contributes to poor clinical features in NSCLC patients and leads to enhanced invasion in A549 cells through regulating miRNA‑203‑mediated epithelial‑mesenchymal transition". Oncology Letters 16, no. 4 (2018): 4480-4488. https://doi.org/10.3892/ol.2018.9187
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