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Article Open Access

A quantitative analysis of the potential biomarkers of non-small cell lung cancer by circulating cell-free DNA

  • Authors:
    • Lirong Wei
    • Wangxi Wu
    • Liming Han
    • Weimo Yu
    • Yuzhen Du
  • View Affiliations / Copyright

    Affiliations: Department of Laboratory Medicine, Shanghai Sixth People's Hospital East Affiliated to Shanghai University of Medicine & Health Sciences, Shanghai 201306, P.R. China, Department of Laboratory Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, East Campus, Shanghai 201306, P.R. China
  • Pages: 4353-4360
    |
    Published online on: July 24, 2018
       https://doi.org/10.3892/ol.2018.9198
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Abstract

The study was conducted to ascertain whether the quantification of circulating cell-free DNA (cfDNA) in serum has value as a diagnostic or for monitoring the progression of non-small cell lung cancer (NSCLC). The serum/plasma cfDNA concentration was quantified by absolute qPCR of long interspersed nuclear element-1 (LINE1) in 60 NSCLC patients and 68 controls in good health. Receiver operating characteristic (ROC) curve analysis was performed to determine the diagnostic utility and cut-off levels of cfDNA, CEA, and CYFRA21-1 in NSCLC patients. Correlations between cfDNA and age, sex, tumour stage and progression-free survival (PFS) were analysed. A follow-up study was conducted on 4 NSCLC patients, and serum cfDNA, CEA, and CYFRA21-1 were quantified throughout disease progression. Serum cfDNA levels were significantly higher in NSCLC patients than those in normal controls. Elevated serum cfDNA concentration was also significantly associated with advanced tumour stage. Serum cfDNA had a ROC area under the curve comparable to that of CEA and CYFRA21-1 for the diagnosis of NSCLC, and the combined cfDNA/CEA/CYFRA21-1 indicator had the highest diagnostic efficiency. Moreover, increased serum cfDNA levels were strongly correlated with tumour progression and poor PFS. This study preliminarily confirmed that cfDNA can monitor disease progression in NSCLC patients, and the lead time was 1-7 months compared with clinical medical imaging. Serum cfDNA may be useful in monitoring NSCLC progression, suggesting that the non-invasive quantification of serum cfDNA by LINE1 qPCR is a viable option for predicting progression and disease severity when repeated invasive tissue biopsy is not possible.
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1 

Ettinger DS, Akerley W, Bepler G, Blum MG, Chang A, Cheney RT, Chirieac LR, D'Amico TA, Demmy TL, Ganti AK, et al; NCCN Non-Small Cell Lung Cancer Panel Members, . Non-small cell lung cancer. J Natl Compr Canc Netw. 8:740–801. 2010. View Article : Google Scholar : PubMed/NCBI

2 

Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J and Jemal A: Global cancer statistics, 2012. CA Cancer J Clin. 65:87–108. 2015. View Article : Google Scholar : PubMed/NCBI

3 

Schiller JH, Harrington D, Belani CP, Langer C, Sandler A, Krook J, Zhu J and Johnson DH; Eastern Cooperative Oncology Group, : Comparison of four chemotherapy regimens for advanced non-small-cell lung cancer. N Engl J Med. 346:92–98. 2002. View Article : Google Scholar : PubMed/NCBI

4 

Stroun M, Lyautey J, Lederrey C, Olson-Sand A and Anker P: About the possible origin and mechanism of circulating DNA apoptosis and active DNA release. Clin Chim Acta. 313:139–142. 2001. View Article : Google Scholar : PubMed/NCBI

5 

Volik S, Alcaide M, Morin RD and Collins C: Cell-free DNA (cfDNA): Clinical significance and utility in cancer shaped by emerging technologies. Mol Cancer Res. 14:898–908. 2016. View Article : Google Scholar : PubMed/NCBI

6 

Jung K, Fleischhacker M and Rabien A: Cell-free DNA in the blood as a solid tumor biomarker—a critical appraisal of the literature. Clin Chim Acta. 411:1611–1624. 2010. View Article : Google Scholar : PubMed/NCBI

7 

Chiappetta C, Anile M, Leopizzi M, Venuta F and Della Rocca C: Use of a new generation of capillary electrophoresis to quantify circulating free DNA in non-small cell lung cancer. Clin Chim Acta. 425:93–96. 2013. View Article : Google Scholar : PubMed/NCBI

8 

Ulivi P, Mercatali L, Casoni GL, Scarpi E, Bucchi L, Silvestrini R, Sanna S, Monteverde M, Amadori D, Poletti V, et al: Multiple marker detection in peripheral blood for NSCLC diagnosis. PLoS One. 8:e574012013. View Article : Google Scholar : PubMed/NCBI

9 

Cargnin S, Canonico PL, Genazzani AA and Terrazzino S: Quantitative analysis of circulating cell-free DNA for correlation with lung cancer survival: A systematic review and meta-analysis. J Thorac Oncol. 12:43–53. 2017. View Article : Google Scholar : PubMed/NCBI

10 

Jiang T, Zhai C, Su C, Ren S and Zhou C: The diagnostic value of circulating cell free DNA quantification in non-small cell lung cancer: A systematic review with meta-analysis. Lung Cancer. 100:63–70. 2016. View Article : Google Scholar : PubMed/NCBI

11 

Sozzi G, Conte D, Leon M, Ciricione R, Roz L, Ratcliffe C, Roz E, Cirenei N, Bellomi M, Pelosi G, et al: Quantification of free circulating DNA as a diagnostic marker in lung cancer. J Clin Oncol. 21:3902–3908. 2003. View Article : Google Scholar : PubMed/NCBI

12 

Devonshire AS, Whale AS, Gutteridge A, Jones G, Cowen S, Foy CA and Huggett JF: Towards standardisation of cell-free DNA measurement in plasma: Controls for extraction efficiency, fragment size bias and quantification. Anal Bioanal Chem. 406:6499–6512. 2014. View Article : Google Scholar : PubMed/NCBI

13 

Jen J, Wu L and Sidransky D: An overview on the isolation and analysis of circulating tumor DNA in plasma and serum. Ann NY Acad Sci. 906:8–12. 2000. View Article : Google Scholar : PubMed/NCBI

14 

Lui YY, Chik KW and Lo YM: Does centrifugation cause the ex vivo release of DNA from blood cells? Clin Chem. 48:2074–2076. 2002.PubMed/NCBI

15 

Jung M, Klotzek S, Lewandowski M, Fleischhacker M and Jung K: Changes in concentration of DNA in serum and plasma during storage of blood samples. Clin Chem. 49:1028–1029. 2003. View Article : Google Scholar : PubMed/NCBI

16 

Eisenhauer EA, Therasse P, Bogaerts J, Schwartz LH, Sargent D, Ford R, Dancey J, Arbuck S, Gwyther S, Mooney M, et al: New response evaluation criteria in solid tumours: revised RECIST guideline (version 1.1). Eur J Cancer. 45:228–247. 2009. View Article : Google Scholar : PubMed/NCBI

17 

Holdhoff M, Schmidt K, Diehl F, Aggrawal N, Angenendt P, Romans K, Edelstein DL, Torbenson M, Kinzler KW, Vogelstein B, et al: Detection of tumor DNA at the margins of colorectal cancer liver metastasis. Clin Cancer Res. 17:3551–3557. 2011. View Article : Google Scholar : PubMed/NCBI

18 

Wang Q, Zheng H, Hu F, Zhang H, Hu Y, Li J, Zhang T, Liu Z, Lu B, Hu A, et al: Serum CYFRA21-1 is correlated with the efficacy of epidermal growth factor receptor-tyrosine kinase inhibitor in non-small cell lung cancer patients harboring EGFR mutations. Zhongguo Fei Ai Za Zhi. 19:550–558. 2016.(In Chinese). PubMed/NCBI

19 

Molina-Vila MA, de-Las-Casas CM, Bertran-Alamillo J, Jordana-Ariza N, González-Cao M and Rosell R: cfDNA analysis from blood in melanoma. Ann Transl Med. 3:3092015.PubMed/NCBI

20 

Chen K, Zhang H, Zhang LN, Ju SQ, Qi J, Huang DF, Li F, Wei Q and Zhang J: Value of circulating cell-free DNA in diagnosis of hepatocelluar carcinoma. World J Gastroenterol. 19:3143–3149. 2013. View Article : Google Scholar : PubMed/NCBI

21 

Cicchillitti L, Corrado G, De Angeli M, Mancini E, Baiocco E, Patrizi L, Zampa A, Merola R, Martayan A, Conti L, et al: Circulating cell-free DNA content as blood based biomarker in endometrial cancer. Oncotarget. 8:115230–115243. 2017. View Article : Google Scholar : PubMed/NCBI

22 

Gormally E, Caboux E, Vineis P and Hainaut P: Circulating free DNA in plasma or serum as biomarker of carcinogenesis: Practical aspects and biological significance. Mutat Res. 635:105–117. 2007. View Article : Google Scholar : PubMed/NCBI

23 

Fawzy A, Sweify KM, El-Fayoumy HM and Nofal N: Quantitative analysis of plasma cell-free DNA and its DNA integrity in patients with metastatic prostate cancer using ALU sequence. J Egypt Natl Cancer Inst. 28:235–242. 2016. View Article : Google Scholar

24 

Lo YM, Tein MS, Lau TK, Haines CJ, Leung TN, Poon PM, Wainscoat JS, Johnson PJ, Chang AM and Hjelm NM: Quantitative analysis of fetal DNA in maternal plasma and serum: Implications for noninvasive prenatal diagnosis. Am J Hum Genet. 62:768–775. 1998. View Article : Google Scholar : PubMed/NCBI

25 

Lee TH, Montalvo L, Chrebtow V and Busch MP: Quantitation of genomic DNA in plasma and serum samples: Higher concentrations of genomic DNA found in serum than in plasma. Transfusion. 41:276–282. 2001. View Article : Google Scholar : PubMed/NCBI

26 

Ma S, Shen L, Qian N and Chen K: The prognostic values of CA125, CA19.9, NSE, AND SCC for stage I NSCLC are limited. Cancer Biomark. 10:155–162. 2011-2012. View Article : Google Scholar

27 

Rosell R, Carcereny E, Gervais R, Vergnenegre A, Massuti B, Felip E, Palmero R, Garcia-Gomez R, Pallares C, Sanchez JM, et al Spanish Lung Cancer Group in collaboration with Groupe Français de Pneumo-Cancérologie and Associazione Italiana Oncologia Toracica, : Erlotinib versus standard chemotherapy as first-line treatment for European patients with advanced EGFR mutation-positive non-small-cell lung cancer (EURTAC): A multicentre, open-label, randomised phase 3 trial. Lancet Oncol. 13:239–246. 2012-2012. View Article : Google Scholar

28 

Schwarzenbach H, Müller V, Stahmann N and Pantel K: Detection and characterization of circulating microsatellite-DNA in blood of patients with breast cancer. Ann NY Acad Sci. 1022:25–32. 2004. View Article : Google Scholar : PubMed/NCBI

29 

Ren N, Qin LX, Tu H, Liu YK, Zhang BH and Tang ZY: The prognostic value of circulating plasma DNA level and its allelic imbalance on chromosome 8p in patients with hepatocellular carcinoma. J Cancer Res Clin Oncol. 132:399–407. 2006. View Article : Google Scholar : PubMed/NCBI

30 

Yoon KA, Park S, Lee SH, Kim JH and Lee JS: Comparison of circulating plasma DNA levels between lung cancer patients and healthy controls. J Mol Diagn. 11:182–185. 2009. View Article : Google Scholar : PubMed/NCBI

31 

Ludovini V, Pistola L, Gregorc V, Floriani I, Rulli E, Piattoni S, Di Carlo L, Semeraro A, Darwish S, Tofanetti FR, et al: Plasma DNA, microsatellite alterations, and p53 tumor mutations are associated with disease-free survival in radically resected non-small cell lung cancer patients: a study of the perugia multidisciplinary team for thoracic oncology. J Thorac Oncol. 3:365–373. 2008. View Article : Google Scholar : PubMed/NCBI

32 

Kamat AA, Baldwin M, Urbauer D, Dang D, Han LY, Godwin A, Karlan BY, Simpson JL, Gershenson DM, Coleman RL, et al: Plasma cell-free DNA in ovarian cancer: An independent prognostic biomarker. Cancer. 116:1918–1925. 2010. View Article : Google Scholar : PubMed/NCBI

33 

Jung K, Stephan C, Lewandowski M, Klotzek S, Jung M, Kristiansen G, Lein M, Loening SA and Schnorr D: Increased cell-free DNA in plasma of patients with metastatic spread in prostate cancer. Cancer Lett. 205:173–180. 2004. View Article : Google Scholar : PubMed/NCBI

34 

Gautschi O, Bigosch C, Huegli B, Jermann M, Marx A, Chassé E, Ratschiller D, Weder W, Joerger M, Betticher DC, et al: Circulating deoxyribonucleic Acid as prognostic marker in non-small-cell lung cancer patients undergoing chemotherapy. J Clin Oncol. 22:4157–4164. 2004. View Article : Google Scholar : PubMed/NCBI

35 

Kumar S, Guleria R, Singh V, Bharti AC, Mohan A and Das BC: Efficacy of circulating plasma DNA as a diagnostic tool for advanced non-small cell lung cancer and its predictive utility for survival and response to chemotherapy. Lung Cancer. 70:211–217. 2010. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wei L, Wu W, Han L, Yu W and Du Y: A quantitative analysis of the potential biomarkers of non-small cell lung cancer by circulating cell-free DNA. Oncol Lett 16: 4353-4360, 2018.
APA
Wei, L., Wu, W., Han, L., Yu, W., & Du, Y. (2018). A quantitative analysis of the potential biomarkers of non-small cell lung cancer by circulating cell-free DNA. Oncology Letters, 16, 4353-4360. https://doi.org/10.3892/ol.2018.9198
MLA
Wei, L., Wu, W., Han, L., Yu, W., Du, Y."A quantitative analysis of the potential biomarkers of non-small cell lung cancer by circulating cell-free DNA". Oncology Letters 16.4 (2018): 4353-4360.
Chicago
Wei, L., Wu, W., Han, L., Yu, W., Du, Y."A quantitative analysis of the potential biomarkers of non-small cell lung cancer by circulating cell-free DNA". Oncology Letters 16, no. 4 (2018): 4353-4360. https://doi.org/10.3892/ol.2018.9198
Copy and paste a formatted citation
x
Spandidos Publications style
Wei L, Wu W, Han L, Yu W and Du Y: A quantitative analysis of the potential biomarkers of non-small cell lung cancer by circulating cell-free DNA. Oncol Lett 16: 4353-4360, 2018.
APA
Wei, L., Wu, W., Han, L., Yu, W., & Du, Y. (2018). A quantitative analysis of the potential biomarkers of non-small cell lung cancer by circulating cell-free DNA. Oncology Letters, 16, 4353-4360. https://doi.org/10.3892/ol.2018.9198
MLA
Wei, L., Wu, W., Han, L., Yu, W., Du, Y."A quantitative analysis of the potential biomarkers of non-small cell lung cancer by circulating cell-free DNA". Oncology Letters 16.4 (2018): 4353-4360.
Chicago
Wei, L., Wu, W., Han, L., Yu, W., Du, Y."A quantitative analysis of the potential biomarkers of non-small cell lung cancer by circulating cell-free DNA". Oncology Letters 16, no. 4 (2018): 4353-4360. https://doi.org/10.3892/ol.2018.9198
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