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Sorbus commixta water extract induces apoptotic cell death via a ROS‑dependent pathway

  • Authors:
    • Seong‑Cheol Moon
    • Hee‑Jung Choi
    • Tae‑Wook Chung
    • Jung‑Hee Lee
    • Syng‑Ook Lee
    • Myeong Ho Jung
    • Byung Joo Kim
    • Jun‑Yong Choi
    • Ki‑Tae Ha
  • View Affiliations / Copyright

    Affiliations: Department of Korean Medical Science, School of Korean Medicine, Pusan National University, Yangsan, Gyeongnam 50612, Republic of Korea, Korean Medical Research Center for Healthy Aging, Pusan National University, Yangsan, Gyeongnam 50612, Republic of Korea, Department of Food Science and Technology, Keimyung University, Daegu 42601, Republic of Korea
    Copyright: © Moon et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4193-4200
    |
    Published online on: July 25, 2018
       https://doi.org/10.3892/ol.2018.9217
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Abstract

The stembark of Sorbus commixta Hedl. has been used for treating asthma, bronchitis, gastritis and edema. However, the anticancer and proapoptotic effects of the water extract of the stembark of S. commixta (SCE) remain unknown. In the present study, it was shown that SCE inhibited the cell viability of the hepatocellular carcinoma cell lines Hep3B and HepG2, and of the colon carcinoma cell line HCT116. DNA content analysis indicated that SCE increased the sub‑G1 population of HCT116 cells. In addition, degradation of nuclear DNA and levels of proapoptotic cascade components, including caspase‑9, caspase‑3 and poly ADP‑ribose polymerase, were augmented by SCE treatment. Mitochondrial membrane potential and the ratio of B‑cell lymphoma‑2 (Bcl‑2)/Bcl‑2‑associated X protein (Bax) were also reduced. Furthermore, SCE increased the expression of proapoptotic proteins, including p21, p27 and p53. Mouse double minute 2 homology, a negative regulator of p53, was cleaved by SCE treatment. Intracellular reactive oxygen species (ROS) production was also increased by SCE treatment. However, the SCE‑induced cytotoxic effects and the increased expression of proapoptotic proteins, including p53 and p21, and reduced Bcl‑2/Bax ratio, could be attenuated by N‑acetyl cysteine, an ROS inhibitor. Taken together, these results indicate that SCE is a potent proapoptotic herbal medicine, which exerts its effects via the ROS‑mediated mitochondrial pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Moon SC, Choi HJ, Chung TW, Lee JH, Lee SO, Jung MH, Kim BJ, Choi JY and Ha KT: Sorbus commixta water extract induces apoptotic cell death via a ROS‑dependent pathway. Oncol Lett 16: 4193-4200, 2018.
APA
Moon, S., Choi, H., Chung, T., Lee, J., Lee, S., Jung, M.H. ... Ha, K. (2018). Sorbus commixta water extract induces apoptotic cell death via a ROS‑dependent pathway. Oncology Letters, 16, 4193-4200. https://doi.org/10.3892/ol.2018.9217
MLA
Moon, S., Choi, H., Chung, T., Lee, J., Lee, S., Jung, M. H., Kim, B. J., Choi, J., Ha, K."Sorbus commixta water extract induces apoptotic cell death via a ROS‑dependent pathway". Oncology Letters 16.4 (2018): 4193-4200.
Chicago
Moon, S., Choi, H., Chung, T., Lee, J., Lee, S., Jung, M. H., Kim, B. J., Choi, J., Ha, K."Sorbus commixta water extract induces apoptotic cell death via a ROS‑dependent pathway". Oncology Letters 16, no. 4 (2018): 4193-4200. https://doi.org/10.3892/ol.2018.9217
Copy and paste a formatted citation
x
Spandidos Publications style
Moon SC, Choi HJ, Chung TW, Lee JH, Lee SO, Jung MH, Kim BJ, Choi JY and Ha KT: Sorbus commixta water extract induces apoptotic cell death via a ROS‑dependent pathway. Oncol Lett 16: 4193-4200, 2018.
APA
Moon, S., Choi, H., Chung, T., Lee, J., Lee, S., Jung, M.H. ... Ha, K. (2018). Sorbus commixta water extract induces apoptotic cell death via a ROS‑dependent pathway. Oncology Letters, 16, 4193-4200. https://doi.org/10.3892/ol.2018.9217
MLA
Moon, S., Choi, H., Chung, T., Lee, J., Lee, S., Jung, M. H., Kim, B. J., Choi, J., Ha, K."Sorbus commixta water extract induces apoptotic cell death via a ROS‑dependent pathway". Oncology Letters 16.4 (2018): 4193-4200.
Chicago
Moon, S., Choi, H., Chung, T., Lee, J., Lee, S., Jung, M. H., Kim, B. J., Choi, J., Ha, K."Sorbus commixta water extract induces apoptotic cell death via a ROS‑dependent pathway". Oncology Letters 16, no. 4 (2018): 4193-4200. https://doi.org/10.3892/ol.2018.9217
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