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Article

Comparison of spatial chromosomal organization between bone marrow and peripheral blood in acute myeloid leukemia

  • Authors:
    • Xueli Tian
    • Yanfang Wang
    • Yu Liu
    • Jun Yin
    • Dieyan Chen
    • Xiaoyan Ke
    • Wanyun Ma
  • View Affiliations / Copyright

    Affiliations: State Key Laboratory of Low‑Dimensional Quantum Physics, Department of Physics, Tsinghua University, Beijing 100084, P.R. China, Department of Hematology, Peking University Third Hospital, Beijing 100191, P.R. China
  • Pages: 4656-4662
    |
    Published online on: July 27, 2018
       https://doi.org/10.3892/ol.2018.9228
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Abstract

Acute myeloid leukemia associated with t(8;21)(q22;q22)/runt related transcription factor (RUNX)1‑RUNX1 translocation partner 1 has been reported to exhibit a favorable outcome. The quantitative polymerase chain reaction is a reliable method for assessing minimal residual disease persistence, and peripheral blood (PB) samples are as informative as bone marrow (BM) samples during follow‑up monitoring. However, few studies have compared the spatial organization of leukemia‑specific chromosomes between BM and PB. In the present study, paired BM and PB samples were extracted from 6 patients with acute myeloid leukaemia‑M2 and compared using three‑dimensional fluorescence in situ hybridization. Cells were classified into three types: Normal, proximal and malignant. Comparisons of proportions (% of all cells) of different cell types revealed no significant difference between BM and PB samples. The relative radial positions (RRPs; d/R) of chromosomes 8 and 21 were consistent for 2/3 of BM and PB samples. The RRPs of chromosomes in proximal pairs were more interior in nuclei compared with chromosomes in normal pairs for BM and PB samples. The consistency of the spatial organization of chromosomes between BM and PB suggests that PB may be an alternative to BM for research and clinical diagnosis.
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Copy and paste a formatted citation
Spandidos Publications style
Tian X, Wang Y, Liu Y, Yin J, Chen D, Ke X and Ma W: Comparison of spatial chromosomal organization between bone marrow and peripheral blood in acute myeloid leukemia. Oncol Lett 16: 4656-4662, 2018.
APA
Tian, X., Wang, Y., Liu, Y., Yin, J., Chen, D., Ke, X., & Ma, W. (2018). Comparison of spatial chromosomal organization between bone marrow and peripheral blood in acute myeloid leukemia. Oncology Letters, 16, 4656-4662. https://doi.org/10.3892/ol.2018.9228
MLA
Tian, X., Wang, Y., Liu, Y., Yin, J., Chen, D., Ke, X., Ma, W."Comparison of spatial chromosomal organization between bone marrow and peripheral blood in acute myeloid leukemia". Oncology Letters 16.4 (2018): 4656-4662.
Chicago
Tian, X., Wang, Y., Liu, Y., Yin, J., Chen, D., Ke, X., Ma, W."Comparison of spatial chromosomal organization between bone marrow and peripheral blood in acute myeloid leukemia". Oncology Letters 16, no. 4 (2018): 4656-4662. https://doi.org/10.3892/ol.2018.9228
Copy and paste a formatted citation
x
Spandidos Publications style
Tian X, Wang Y, Liu Y, Yin J, Chen D, Ke X and Ma W: Comparison of spatial chromosomal organization between bone marrow and peripheral blood in acute myeloid leukemia. Oncol Lett 16: 4656-4662, 2018.
APA
Tian, X., Wang, Y., Liu, Y., Yin, J., Chen, D., Ke, X., & Ma, W. (2018). Comparison of spatial chromosomal organization between bone marrow and peripheral blood in acute myeloid leukemia. Oncology Letters, 16, 4656-4662. https://doi.org/10.3892/ol.2018.9228
MLA
Tian, X., Wang, Y., Liu, Y., Yin, J., Chen, D., Ke, X., Ma, W."Comparison of spatial chromosomal organization between bone marrow and peripheral blood in acute myeloid leukemia". Oncology Letters 16.4 (2018): 4656-4662.
Chicago
Tian, X., Wang, Y., Liu, Y., Yin, J., Chen, D., Ke, X., Ma, W."Comparison of spatial chromosomal organization between bone marrow and peripheral blood in acute myeloid leukemia". Oncology Letters 16, no. 4 (2018): 4656-4662. https://doi.org/10.3892/ol.2018.9228
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