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Dextran sulfate inhibition on human gastric cancer cells invasion, migration and epithelial‑mesenchymal transformation

  • Authors:
    • Yuanyi Xu
    • Xiu Jin
    • Yunning Huang
    • Juan Wang
    • Xiaofei Wang
    • Honghong Wang
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region 750001, P.R. China, Department of Pathology, Affiliated Hospital of Jining Medical University, Jining, Shandong 272029, P.R. China, Department of Gastrointestinal Surgery, Ningxia People's Hospital, Yinchuan, Ningxia Hui Autonomous Region 750021, P.R. China, Department of Pathology, Ningxia People's Hospital, Yinchuan, Ningxia Hui Autonomous Region 750021, P.R. China
    Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5041-5049
    |
    Published online on: August 2, 2018
       https://doi.org/10.3892/ol.2018.9251
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Abstract

The objective of the present study was to observe the influence of dextran sulfate (DS) on the proliferation, invasion and migration of AGS, BGC‑23, GES‑1, MGC‑803 and SGC‑7901 cells. Additionally, the possible inhibition mechanism of DS on BGC‑823 cells epithelial‑mesenchymal transition (EMT) was explored. The cells in the control and experimental group were treated with PBS and DS respectively. The effect of DS on the invasion and migration of these five types of cells were investigated using Transwell invasion and migration assays. Immunocytochemistry, western blotting and reverse transcription‑polymerase chain reaction (RT‑PCR) assays were used to measure gene and protein expression of hypoxia‑inducible factor 1α (HIF1‑a) and EMT associated factors [Twist, E‑cadherin, N‑cadherin and β‑catenin] of BGC‑823 cells. According to the results of CCK‑8, DS significantly decreased the proliferation of AGS, SGC‑7901 and BGC‑823 cells to different extents, but there were no notable differences for MGC‑803 cells. Transwell migration and invasion results demonstrated that, compared with the control group, DS reduced the migration and invasion of every types of cells to different extents, and the inhibition to BGC‑823 cells invasion is the most notably. Immunofluorescence, RT‑PCR and western blot analysis results indicated that HIF‑1α, Twist and N‑cad expressions levels had different degrees of reduction in the experimental group following DS treatment; however, the expression level of E‑cad had increased. In conclusion, DS inhibited the proliferation of AGS, BGC‑823, SGC‑7901 and GES‑1 cells, the inhibition degree may be associated with the differentiation degree of every cancer cell, the higher the differentiation degree, the stronger the inhibition. DS inhibited migration and invasion of the five types of gastric cancer cells in different degree. DS may inhibit EMT of BGC‑823 by inhibiting Wnt signaling.
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View References

1 

Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA-Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar : PubMed/NCBI

2 

Shen L, Shan YS, Hu HM, Price TJ, Sirohi B, Yeh KH, Yang YH, Sano T, Yang HK, Zhang X, et al: Management of gastric cancer in Asia: Resource-stratified guidelines. Lancet Oncol. 14:e535–e547. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Takagi T, Sakakura C, Kin S, Nakase Y, Fukuda K, Shimomura K, Ito T, Fujiyama J, Yamasaki J, Tsujimoto H, et al: Dextran sulfate suppresses cell adhesion and cell cycle progression of melanoma cells. Anticancer Res. 25:895–902. 2005.PubMed/NCBI

4 

Xu YY, Huang YN, Wang HH and Liu Y: Inhibition of the peritoneal metastasis of human gastric cancer cells by dextran sulphate in vivo and in vitro. Oncol Lett. 11:2384–2390. 2016. View Article : Google Scholar : PubMed/NCBI

5 

Xu Y, Jin X, Huang Y, Dong J, Wang H, Wang X and Cao X: Inhibition of peritoneal metastasis of human gastric cancer cells by dextran sulphate through the reduction in HIF-1α and ITGβ1 expression. Oncol Rep. 35:2624–2634. 2016. View Article : Google Scholar : PubMed/NCBI

6 

Gurzu S, Turdean S, Kovecsi A, Contac AO and Jung I: Epithelial-mesenchymal, mesenchymal-epithelial, and endothelial-mesenchymal transitions in malignant tumors: An update. World J Clin Cases. 3:393–404. 2015. View Article : Google Scholar : PubMed/NCBI

7 

Daly CS, Flemban A, Shafei M, Conway ME, Qualtrough D and Dean SJ: Hypoxia modulates the stem cell population and induces EMT in the MCF-10A breast epithelial cell line. Oncol Rep. 39:483–490. 2018.PubMed/NCBI

8 

Noman MZ, Messai Y, Carré T, Akalay I, Méron M, Janji B, Hasmim M and Chouaib S: Microenvironmental hypoxia orchestrating the cell stroma cross talk, tumor progression and antitumor response. Crit Rev Immunol. 31:357–377. 2011. View Article : Google Scholar : PubMed/NCBI

9 

Bao B, Wang Z, Ali S, Kong D, Li Y, Ahmad A, Banerjee S, Azmi AS, Miele L and Sarkar FH: Notch-1 induces epithelial-mesenchymal transition consistent with cancer stem cell phenotype in pancreatic cancer cells. Cancer Lett. 307:26–36. 2011. View Article : Google Scholar : PubMed/NCBI

10 

Goggins BJ, Chaney C, Radford-Smith GL, Horvat JC and Keely S: Hypoxia and integrin-mediated epithelial restitution during mucosal inflammation. Front Immunol. 4:2722013. View Article : Google Scholar : PubMed/NCBI

11 

Peng Z, Wang CX, Fang EH, Wang GB and Tong Q: Role of epithelial-mesenchymal transition in gastric cancer initiation and progression. World J Gastroenterol. 20:5403–5410. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Fu F, Hu H, Yang S and Liang X: Effects of TIN2 on telomeres and chromosomes in the human gastric epithelial cell line GES-1. Oncol Lett. 15:5161–5166. 2018.PubMed/NCBI

13 

Goto A, Nishikawa J, Hideura E, Ogawa R, Nagao M, Sasaki S, Kawasato R, Hashimoto S, Okamoto T, Ogihara H, et al: Lymph node metastasis can be determined by just tumor depth and lymphovascular invasion in early gastric cancer patients after endoscopic submucosal dissection. Eur J Gastroenterol Hepatol. 29:1346–1350. 2017. View Article : Google Scholar : PubMed/NCBI

14 

Hou XH: The expression of HORMAD2 and the effects of it on proliferation and apoptosis on gastric cancer cell (D). Lanzhou Univ. 2015.

15 

Weili Xu: The effect of lysine (K)-specific demethylase 1A on invasion and metastasis of gastric cancer and its associated mechanism (D). Central South Univ. 2014.

16 

Zheng L, Hu X, Huang Y, Xu G, Yang J and Li L: In vivo bioengineered ovarian tumors based on collagen, matrigel, alginate and agarose hydrogels: A comparative study. Biomed Mater. 10:0150162015. View Article : Google Scholar : PubMed/NCBI

17 

Cheng XX, Wang ZC, Chen XY, Sun Y, Kong QY, Liu J, Gao X, Guan HW and Li H: Frequent loss of membranous E-cadherin in gastric cancers: A cross-talk with Wnt in determining the fate of beta-catenin. Clin Exp Metastasis. 22:85–93. 2005. View Article : Google Scholar : PubMed/NCBI

18 

Dong Li: Expression of β-catenin-the Central Regulation Molecule of the Wnt Signaling Pathways in gastric cancer and its significance (D). Nanchang Univ. 2014.

19 

Zhang Z, Bu X, Chen H, Wang Q and Sha W: Bmi-1 promotes the invasion and migration of colon cancer stem cells through the downregulation of E-cadherin. Int J Mol Med. 38:1199–1207. 2016. View Article : Google Scholar : PubMed/NCBI

20 

Huelsken J and Behrens J: The Wnt signalling pathway. J Cell Sci. 115:3977–3978. 2002. View Article : Google Scholar : PubMed/NCBI

21 

Kalluri R and Weinberg R: The basics of epithelial-mesenchymal transition. J Clin Invest. 119:1420–1428. 2009. View Article : Google Scholar : PubMed/NCBI

22 

Ashaie MA and Chowdhury EH: Cadherins: The superfamily critically involved in breast cancer. Curr Pharm Des. 22:616–638. 2016. View Article : Google Scholar : PubMed/NCBI

23 

Zhang X, Liu G, Kang Y, Dong Z, Qian Q and Ma X: N-cadherin expression is associated with acquisition of EMT phenotype and with enhanced invasion in erlotinib-resistant lung cancer cell lines. PLoS One. 8:e576922013. View Article : Google Scholar : PubMed/NCBI

24 

Wu S, Luo Z, Yu PJ, Xie H and He YW: Suberoylanilide hydroxamic acid (SAHA) promotes the epithelial mesenchymal transition of triple negative breast cancer cells via HDAC8/FOXA1 signals. Biol Chem. 397:75–83. 2016. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Xu Y, Jin X, Huang Y, Wang J, Wang X and Wang H: Dextran sulfate inhibition on human gastric cancer cells invasion, migration and epithelial‑mesenchymal transformation. Oncol Lett 16: 5041-5049, 2018.
APA
Xu, Y., Jin, X., Huang, Y., Wang, J., Wang, X., & Wang, H. (2018). Dextran sulfate inhibition on human gastric cancer cells invasion, migration and epithelial‑mesenchymal transformation. Oncology Letters, 16, 5041-5049. https://doi.org/10.3892/ol.2018.9251
MLA
Xu, Y., Jin, X., Huang, Y., Wang, J., Wang, X., Wang, H."Dextran sulfate inhibition on human gastric cancer cells invasion, migration and epithelial‑mesenchymal transformation". Oncology Letters 16.4 (2018): 5041-5049.
Chicago
Xu, Y., Jin, X., Huang, Y., Wang, J., Wang, X., Wang, H."Dextran sulfate inhibition on human gastric cancer cells invasion, migration and epithelial‑mesenchymal transformation". Oncology Letters 16, no. 4 (2018): 5041-5049. https://doi.org/10.3892/ol.2018.9251
Copy and paste a formatted citation
x
Spandidos Publications style
Xu Y, Jin X, Huang Y, Wang J, Wang X and Wang H: Dextran sulfate inhibition on human gastric cancer cells invasion, migration and epithelial‑mesenchymal transformation. Oncol Lett 16: 5041-5049, 2018.
APA
Xu, Y., Jin, X., Huang, Y., Wang, J., Wang, X., & Wang, H. (2018). Dextran sulfate inhibition on human gastric cancer cells invasion, migration and epithelial‑mesenchymal transformation. Oncology Letters, 16, 5041-5049. https://doi.org/10.3892/ol.2018.9251
MLA
Xu, Y., Jin, X., Huang, Y., Wang, J., Wang, X., Wang, H."Dextran sulfate inhibition on human gastric cancer cells invasion, migration and epithelial‑mesenchymal transformation". Oncology Letters 16.4 (2018): 5041-5049.
Chicago
Xu, Y., Jin, X., Huang, Y., Wang, J., Wang, X., Wang, H."Dextran sulfate inhibition on human gastric cancer cells invasion, migration and epithelial‑mesenchymal transformation". Oncology Letters 16, no. 4 (2018): 5041-5049. https://doi.org/10.3892/ol.2018.9251
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