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Article Open Access

D Rhamnose β‑Hederin against human breast cancer by reducing tumor‑derived exosomes

  • Authors:
    • Wei‑Xian Chen
    • Lin Cheng
    • Meng Pan
    • Qi Qian
    • Yu‑Lan Zhu
    • Ling‑Yun Xu
    • Qiang Ding
  • View Affiliations / Copyright

    Affiliations: Department of Breast Surgery, The Affiliated Changzhou No. 2 People's Hospital with Nanjing Medical University, Changzhou, Jiangsu 213000, P.R. China, Department of Pediatrics, The Affiliated Changzhou No. 1 People's Hospital with Suzhou University, Changzhou, Jiangsu 213000, P.R. China, Department of Breast Surgery, The First Affiliated Hospital with Nanjing Medical University, Nanjing, Jiangsu 210000, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5172-5178
    |
    Published online on: August 2, 2018
       https://doi.org/10.3892/ol.2018.9254
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Abstract

D Rhamnose β‑hederin (DRβ‑H), a novel oleanane‑type triterpenoid saponin isolated from the traditional Chinese medicinal plant Clematis ganpiniana, has been demonstrated to be effective against various types of tumor. However, the exact role of DRβ‑H on breast cancer remains largely unresolved. In the present study, it was observed that DRβ‑H exhibited anti‑proliferative and pro‑apoptotic activity in human breast cancer cells (MCF‑7/S). DRβ‑H was able to inhibit exosome secretion, and the level of exosomes was positively associated with cell growth after absorption and internalization by target breast cancer cells. By analyzing the miRNA profiles of exosomes and MCF‑7/S, it was identified that several miRNAs were detected exclusively in exosomes. Knockdown of the top five exosomal miRNAs and an MCF‑7/S proliferation assay indicated that exosomal miR‑130a and miR‑425 may enhance MCF‑7/S cell viability. Target gene prediction and pathway analysis revealed the involvement of miR‑130a and miR‑425 in pathways associated with malignant cell proliferation. These results demonstrated that DRβ‑H inhibited MCF‑7/S cell growth through reducing exosome release.
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Copy and paste a formatted citation
Spandidos Publications style
Chen WX, Cheng L, Pan M, Qian Q, Zhu YL, Xu LY and Ding Q: D Rhamnose β‑Hederin against human breast cancer by reducing tumor‑derived exosomes. Oncol Lett 16: 5172-5178, 2018.
APA
Chen, W., Cheng, L., Pan, M., Qian, Q., Zhu, Y., Xu, L., & Ding, Q. (2018). D Rhamnose β‑Hederin against human breast cancer by reducing tumor‑derived exosomes. Oncology Letters, 16, 5172-5178. https://doi.org/10.3892/ol.2018.9254
MLA
Chen, W., Cheng, L., Pan, M., Qian, Q., Zhu, Y., Xu, L., Ding, Q."D Rhamnose β‑Hederin against human breast cancer by reducing tumor‑derived exosomes". Oncology Letters 16.4 (2018): 5172-5178.
Chicago
Chen, W., Cheng, L., Pan, M., Qian, Q., Zhu, Y., Xu, L., Ding, Q."D Rhamnose β‑Hederin against human breast cancer by reducing tumor‑derived exosomes". Oncology Letters 16, no. 4 (2018): 5172-5178. https://doi.org/10.3892/ol.2018.9254
Copy and paste a formatted citation
x
Spandidos Publications style
Chen WX, Cheng L, Pan M, Qian Q, Zhu YL, Xu LY and Ding Q: D Rhamnose β‑Hederin against human breast cancer by reducing tumor‑derived exosomes. Oncol Lett 16: 5172-5178, 2018.
APA
Chen, W., Cheng, L., Pan, M., Qian, Q., Zhu, Y., Xu, L., & Ding, Q. (2018). D Rhamnose β‑Hederin against human breast cancer by reducing tumor‑derived exosomes. Oncology Letters, 16, 5172-5178. https://doi.org/10.3892/ol.2018.9254
MLA
Chen, W., Cheng, L., Pan, M., Qian, Q., Zhu, Y., Xu, L., Ding, Q."D Rhamnose β‑Hederin against human breast cancer by reducing tumor‑derived exosomes". Oncology Letters 16.4 (2018): 5172-5178.
Chicago
Chen, W., Cheng, L., Pan, M., Qian, Q., Zhu, Y., Xu, L., Ding, Q."D Rhamnose β‑Hederin against human breast cancer by reducing tumor‑derived exosomes". Oncology Letters 16, no. 4 (2018): 5172-5178. https://doi.org/10.3892/ol.2018.9254
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