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Article

ATPase inhibitory factor 1 inhibition improves the antitumor of YC‑1 against hepatocellular carcinoma

  • Authors:
    • Xuemei Ding
    • Jian Kong
    • Wenlei Xu
    • Shuying Dong
    • Yingrui Du
    • Changyu Yao
    • Jun Gao
    • Shan Ke
    • Shaohong Wang
    • Wenbing Sun
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary Surgery, Beijing Chao‑yang Hospital, Capital Medical University, Beijing 100043, P.R. China
    Copyright: © Ding et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5230-5236
    |
    Published online on: August 3, 2018
       https://doi.org/10.3892/ol.2018.9266
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Abstract

YC‑1 is a synthetic compound, which serves as a hypoxia‑inducible factor 1‑α inhibitor or sensitizer to enhance the effect of chemotherapy. Previous studies have revealed the anti‑cancer effects of YC‑1 in various types of cancer, including hepatocellular carcinoma (HCC). ATPase inhibitory factor 1 (IF1) is upregulated in a number of human carcinomas and regulates mitochondrial bioenergetics and structure. However, whether IF1 is involved in the antitumor effects of YC‑1 against HCC remains unclear. The present study examined the function of IF1 in HCC and its potential role in YC‑1 effects within HCC cells. MTT, colony formation and Transwell assays revealed that IF1 overexpression promoted proliferation, colony formation and invasion of HCC cells, while IF1 downregulation had the opposite effects. Overexpression of IF1 reversed the inhibitory effects of YC‑1 on Huh7 cell growth and invasion activities, while downregulation of IF1 increased the sensitivity of HCCLM3 cells to YC‑1. YC‑1 treatment of HCCLM3 and Huh7 cells reduced the levels of phosphorylated (p‑) signal transducer and activator of transcription 3 (STAT3) and IF1, and increased the expression of E‑cadherin. IF1 knockdown resulted in decreased p‑STAT3 levels and increased E‑cadherin expression, while IF1 overexpression increased p‑STAT3 levels and reduced the expression of E‑cadherin. The present study demonstrated that the inhibition of IF1 improves the antitumor effects of YC‑1 in HCC cells. These findings support the clinical strategy of combining YC‑1 and an IF1 inhibitor for the treatment of HCC.
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Copy and paste a formatted citation
Spandidos Publications style
Ding X, Kong J, Xu W, Dong S, Du Y, Yao C, Gao J, Ke S, Wang S, Sun W, Sun W, et al: ATPase inhibitory factor 1 inhibition improves the antitumor of YC‑1 against hepatocellular carcinoma. Oncol Lett 16: 5230-5236, 2018.
APA
Ding, X., Kong, J., Xu, W., Dong, S., Du, Y., Yao, C. ... Sun, W. (2018). ATPase inhibitory factor 1 inhibition improves the antitumor of YC‑1 against hepatocellular carcinoma. Oncology Letters, 16, 5230-5236. https://doi.org/10.3892/ol.2018.9266
MLA
Ding, X., Kong, J., Xu, W., Dong, S., Du, Y., Yao, C., Gao, J., Ke, S., Wang, S., Sun, W."ATPase inhibitory factor 1 inhibition improves the antitumor of YC‑1 against hepatocellular carcinoma". Oncology Letters 16.4 (2018): 5230-5236.
Chicago
Ding, X., Kong, J., Xu, W., Dong, S., Du, Y., Yao, C., Gao, J., Ke, S., Wang, S., Sun, W."ATPase inhibitory factor 1 inhibition improves the antitumor of YC‑1 against hepatocellular carcinoma". Oncology Letters 16, no. 4 (2018): 5230-5236. https://doi.org/10.3892/ol.2018.9266
Copy and paste a formatted citation
x
Spandidos Publications style
Ding X, Kong J, Xu W, Dong S, Du Y, Yao C, Gao J, Ke S, Wang S, Sun W, Sun W, et al: ATPase inhibitory factor 1 inhibition improves the antitumor of YC‑1 against hepatocellular carcinoma. Oncol Lett 16: 5230-5236, 2018.
APA
Ding, X., Kong, J., Xu, W., Dong, S., Du, Y., Yao, C. ... Sun, W. (2018). ATPase inhibitory factor 1 inhibition improves the antitumor of YC‑1 against hepatocellular carcinoma. Oncology Letters, 16, 5230-5236. https://doi.org/10.3892/ol.2018.9266
MLA
Ding, X., Kong, J., Xu, W., Dong, S., Du, Y., Yao, C., Gao, J., Ke, S., Wang, S., Sun, W."ATPase inhibitory factor 1 inhibition improves the antitumor of YC‑1 against hepatocellular carcinoma". Oncology Letters 16.4 (2018): 5230-5236.
Chicago
Ding, X., Kong, J., Xu, W., Dong, S., Du, Y., Yao, C., Gao, J., Ke, S., Wang, S., Sun, W."ATPase inhibitory factor 1 inhibition improves the antitumor of YC‑1 against hepatocellular carcinoma". Oncology Letters 16, no. 4 (2018): 5230-5236. https://doi.org/10.3892/ol.2018.9266
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