Hsp27 regulates epithelial mesenchymal transition, metastasis and proliferation in colorectal carcinoma

Han,Lu; Jiang,Yong; Han,Dongxing; Tan,Weilin

doi:10.3892/ol.2018.9286

Hsp27 regulates epithelial mesenchymal transition, metastasis and proliferation in colorectal carcinoma

Authors:
- Lu Han
- Yong Jiang
- Dongxing Han
- Weilin Tan
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Affiliations: Department of Surgery, Shanghai Jiangong Hospital, Shanghai 200083, P.R. China
Published online on: August 9, 2018 https://doi.org/10.3892/ol.2018.9286
Pages: 5309-5316

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Abstract

The primary factor associated with poor survival rate in patients with colorectal carcinoma (CRC) is the presence of metastasis. The underlying molecular mechanisms of CRC metastasis are yet to be fully elucidated. The present study investigated the function of heat shock protein 27 (Hsp27) on the invasion and proliferation of CRC cells. The clinical significance of Hsp27 was evaluated using tissue microarray analysis (n=81). Invasion and metastasis assays were used to determine the function of Hsp27 in CRC metastasis in vitro and in vivo using RNA interference and the ectopic expression of Hsp27. The upregulation of Hsp27 has been frequently identified in CRC tissues. Patients with CRC and a high expression level of Hsp27 have a reduced overall survival rate. Silencing Hsp27 inhibited the growth and invasion of CRC cells in vitro and in vivo, whereas ectopic overexpression of Hsp27 promoted the proliferation and invasion of CRC cells in vitro. Furthermore, depletion of Hsp27 expression inhibited the epithelial‑to‑mesenchymal transition (EMT), whilst ectopic overexpression of Hsp27 induced EMT. The results of the present study indicated that Hsp27 serves an important function in the aggressiveness of CRC through inducing EMT. Hsp27 suppression may represent a potential therapeutic option for the suppression of CRC progression.

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