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Article Open Access

An analysis of the expression and function of myeloid differentiation factor 88 in human osteosarcoma

  • Authors:
    • Jun Chen
    • Jian He
    • Yue Yang
    • Jiannong Jiang
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, The Affiliated Yixing Hospital of Jiangsu University, Yixing, Jiangsu 214200, P.R. China, Department of Surgery, The Third Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210001, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4929-4936
    |
    Published online on: August 13, 2018
       https://doi.org/10.3892/ol.2018.9297
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Abstract

The aim of the present study was to investigate the expression and function of myeloid differentiation factor 88 (MyD88) in osteosarcoma. Immunohistochemical staining was used to detect MyD88 protein in osteosarcoma tissues and matched normal bone tissues. The association between MyD88 expression and the clinical characteristics of patients with osteosarcoma was analyzed. Furthermore, survival analysis of patients with osteosarcoma was performed to study the association between MyD88 expression and patient prognosis. Finally, the effect of the MyD88 inhibitor, ST2825, on the proliferation and apoptosis of the human osteosarcoma cell line U2OS was examined. Additionally, cell proliferation, invasion and apoptosis were examined using an MTT assay, Transwell assay and Annexin V‑fluorescein isothiocyanate staining kit, respectively. The expression of proteins associated with the NF‑κB signaling pathway was analyzed by western blotting. The positive expression rate of MyD88 in osteosarcoma and normal bone tissues was 71.4 and 6.1%, respectively. Statistical analysis demonstrated that MyD88 was not associated with gender, age, histological type or tumor location, but that it was associated with Enneking stage and tumor metastasis (P<0.05). According to the survival analysis, patients with osteosarcoma in the high MyD88 expression group displayed a reduced overall survival rate (P<0.05). Furthermore, inhibition of MyD88 by ST2825 in U2OS cells resulted in a marked decrease in cellular proliferation and migration, and an increase in the rate of apoptosis (P<0.05). Notably, ST2825 significantly decreased cyclin D1, matrix metallopeptidase‑9 and nucleus p65 expression, but increased cleaved‑caspase 3 expression in ST2825‑treated U2OS cells (P<0.05). The results of the present study indicated that MyD88 expression is associated with the progression of osteosarcoma and may be a potential therapeutic target for the treatment of osteosarcoma.
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1 

Bishop MW, Janeway KA and Gorlick R: Future directions in the treatment of osteosarcoma. Curr Opin Pediatr. 28:26–33. 2016. View Article : Google Scholar : PubMed/NCBI

2 

Ferrari S and Serra M: An update on chemotherapy for osteosarcoma. Expert Opin Pharmacother. 16:2727–2736. 2015. View Article : Google Scholar : PubMed/NCBI

3 

Xiang F, Ni Z, Zhan Y, Kong Q, Xu J, Jiang J, Wu R and Kang X: Increased expression of MyD88 and association with paclitaxel resistance in breast cancer. Tumour Biol. 37:6017–6025. 2016. View Article : Google Scholar : PubMed/NCBI

4 

Zhu Y, Huang JM, Zhang GN, Zha X and Deng BF: Prognostic significance of MyD88 expression by human epithelial ovarian carcinoma cells. J Transl Med. 10:772012. View Article : Google Scholar : PubMed/NCBI

5 

Liang B, Chen R, Wang T, Cao L, Liu Y, Yin F, Zhu M, Fan X, Liang Y, Zhang L, et al: Myeloid differentiation factor 88 promotes growth and metastasis of human hepatocellular carcinoma. Clin Cancer Res. 19:2905–2916. 2013. View Article : Google Scholar : PubMed/NCBI

6 

Salcedo R, Worschech A, Cardone M, Jones Y, Gyulai Z, Dai RM, Wang E, Ma W, Haines D, O'Huigin C, et al: MyD88-mediated signaling prevents development of adenocarcinomas of the colon: Role of interleukin 18. J Exp Med. 207:1625–1636. 2010. View Article : Google Scholar : PubMed/NCBI

7 

Kuo AH and Scheeren FA: Cell-intrinsic TLR2/MyD88 pathway in breast and colon cancer. Cell Cycle. 13:3785–3786. 2014. View Article : Google Scholar : PubMed/NCBI

8 

Di Padova F, Quesniaux VFJ and Ryffel B: MyD88 as a therapeutic target for inflammatory lung diseases. Expert Opin Ther Targets. 22:401–408. 2018. View Article : Google Scholar : PubMed/NCBI

9 

Tang QL, Xie XB, Wang J, Chen Q, Han AJ, Zou CY, Yin JQ, Liu DW, Liang Y, Zhao ZQ, et al: Glycogen synthase kinase-3β, NF-κB signaling, and tumorigenesis of human osteosarcoma. J Natl Cancer Inst. 104:749–763. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Xu HY, Fang W, Huang ZW, Lu JC, Wang YQ, Tang QL, Song GH, Kang Y, Zhu XJ, Zou CY, et al: Metformin reduces SATB2-mediated osteosarcoma stem cell-like phenotype and tumor growth via inhibition of N-cadherin/NF-kB signaling. Eur Rev Med Pharmacol Sci. 21:4516–4528. 2017.PubMed/NCBI

11 

Lu Y, Li F, Xu T and Sun J: Tetrandrine prevents multidrug resistance in the osteosarcoma cell line, U-2OS, by preventing Pgp overexpression through the inhibition of NF-κB signaling. Int J Mol Med. 39:993–1000. 2017. View Article : Google Scholar : PubMed/NCBI

12 

Loiarro M, Capolunghi F, Fantò N, Gallo G, Campo S, Arseni B, Carsetti R, Carminati P, De Santis R, Ruggiero V and Sette C: Pivotal Advance: Inhibition of MyD88 dimerization and recruitment of IRAK1 and IRAK4 by a novel peptidomimetic compound. J Leukoc Biol. 82:801–810. 2017. View Article : Google Scholar

13 

Zhang Y, Meng W and Cui H: LncRNA CBR3-AS1 predicts unfavorable prognosis and promotes tumorigenesis in osteosarcoma. Biomed Pharmacother. 102:169–174. 2018. View Article : Google Scholar : PubMed/NCBI

14 

Zhou Y, Zhang W, Tang F, Luo Y, Min L, Zhang W, Shi R, Duan H and Tu C: A case report of apatinib in treating osteosarcoma with pulmonary metastases. Medicine (Baltimore). 96:e65782017. View Article : Google Scholar : PubMed/NCBI

15 

Chen X, Zhao F, Zhang H, Zhu Y, Wu K and Tan G: Significance of TLR4/MyD88 expression in breast cancer. Int J Clin Exp Pathol. 8:7034–7039. 2015.PubMed/NCBI

16 

Kfoury A, Virard F, Renno T and Coste I: Dual function of MyD88 in inflammation and oncogenesis: Implications for therapeutic intervention. Curr Opin Oncol. 26:86–91. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Deng Y, Sun J and Zhang LD: Effect of ST2825 on the proliferation and apoptosis of human hepatocellular carcinoma cells. Genet Mol Res. 15:150168262016. View Article : Google Scholar : PubMed/NCBI

18 

Shiratori E, Itoh M and Tohda S: MYD88 inhibitor ST2825 suppresses the growth of lymphoma and leukaemia cells. Anticancer Res. 37:6203–6209. 2017.PubMed/NCBI

19 

Warner N and Núñez G: MyD88: A critical adaptor protein in innate immunity signal transduction. J Immunol. 190:3–4. 2013. View Article : Google Scholar : PubMed/NCBI

20 

Cervantes JL: MyD88 in Mycobacterium tuberculosis infection. Med Microbiol Immunol. 206:187–193. 2017. View Article : Google Scholar : PubMed/NCBI

21 

Deguine J and Barton GM: MyD88: A central player in innate immune signaling. F1000Prime Rep. 6:972014. View Article : Google Scholar : PubMed/NCBI

22 

Chandhanayingyong C, Kim Y, Staples JR, Hahn C and Lee FY: MAPK/ERK signaling in osteosarcomas, ewing sarcomas and chondrosarcomas: Therapeutic implications and future directions. Sarcoma. 2012:4048102012. View Article : Google Scholar : PubMed/NCBI

23 

Meng Q, Zheng M, Liu H, Song C, Zhang W, Yan J, Qin L and Liu X: TRAF6 regulates proliferation, apoptosis, and invasion of osteosarcoma cell. Mol Cell Biochem. 371:177–186. 2012. View Article : Google Scholar : PubMed/NCBI

24 

Miwa S, Sugimoto N, Yamamoto N, Shirai T, Nishida H, Hayashi K, Kimura H, Takeuchi A, Igarashi K, Yachie A and Tsuchiya H: Caffeine induces apoptosis of osteosarcoma cells by inhibiting AKT/mTOR/S6K, NF-κB and MAPK pathways. Anticancer Res. 32:3643–3649. 2012.PubMed/NCBI

25 

Zhao Z, Wu MS, Zou C, Tang Q, Lu J, Liu D, Wu Y, Yin J, Xie X, Shen J, et al: Downregulation of MCT1 inhibits tumor growth, metastasis and enhances chemotherapeutic efficacy in osteosarcoma through regulation of the NF-κB pathway. Cancer Lett. 342:150–158. 2014. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Chen J, He J, Yang Y and Jiang J: An analysis of the expression and function of myeloid differentiation factor 88 in human osteosarcoma. Oncol Lett 16: 4929-4936, 2018.
APA
Chen, J., He, J., Yang, Y., & Jiang, J. (2018). An analysis of the expression and function of myeloid differentiation factor 88 in human osteosarcoma. Oncology Letters, 16, 4929-4936. https://doi.org/10.3892/ol.2018.9297
MLA
Chen, J., He, J., Yang, Y., Jiang, J."An analysis of the expression and function of myeloid differentiation factor 88 in human osteosarcoma". Oncology Letters 16.4 (2018): 4929-4936.
Chicago
Chen, J., He, J., Yang, Y., Jiang, J."An analysis of the expression and function of myeloid differentiation factor 88 in human osteosarcoma". Oncology Letters 16, no. 4 (2018): 4929-4936. https://doi.org/10.3892/ol.2018.9297
Copy and paste a formatted citation
x
Spandidos Publications style
Chen J, He J, Yang Y and Jiang J: An analysis of the expression and function of myeloid differentiation factor 88 in human osteosarcoma. Oncol Lett 16: 4929-4936, 2018.
APA
Chen, J., He, J., Yang, Y., & Jiang, J. (2018). An analysis of the expression and function of myeloid differentiation factor 88 in human osteosarcoma. Oncology Letters, 16, 4929-4936. https://doi.org/10.3892/ol.2018.9297
MLA
Chen, J., He, J., Yang, Y., Jiang, J."An analysis of the expression and function of myeloid differentiation factor 88 in human osteosarcoma". Oncology Letters 16.4 (2018): 4929-4936.
Chicago
Chen, J., He, J., Yang, Y., Jiang, J."An analysis of the expression and function of myeloid differentiation factor 88 in human osteosarcoma". Oncology Letters 16, no. 4 (2018): 4929-4936. https://doi.org/10.3892/ol.2018.9297
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