Identification of a novel splice variant of the human musashi‑1 gene

  • Authors:
    • Lin Ma
    • Yating Shan
    • Heliang Ma
    • Ihab Elshoura
    • Muhammad Nafees
    • Kaiyong Yang
    • Wu Yin
  • View Affiliations

  • Published online on: August 13, 2018     https://doi.org/10.3892/ol.2018.9300
  • Pages: 5441-5448
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Abstract

Musashi‑1 (Msi1) is an evolutionarily conserved RNA‑binding protein that has been reported to be the key regulator in malignancies and with involvement in cancer stemness. In the present study, a novel Msi1 transcript variant generated by alternative splicing was identified and termed Msi1 variant 2. This variant was observed to be ubiquitously expressed in cancerous and non‑cancerous cells compared with its wild‑type variant, which is preferentially expressed in cancer cells. Notably, the expression levels of Msi1 variant 2 were inversely associated with the protein expression levels of Msi1 in various cancer cells. This naturally truncated variant contains 899 nucleotides and a skipping event of exons 3 and 4, which leads to the emergence of a premature TGA stop codon in exon 5. The present results also demonstrated that hypoxia increased the resistance of H460 cells to cisplatin by suppressing the exon 3 and 4 skipping event of Msi1. In summary, the present study identified a novel splice variant of Msi1 lacking two complete RNA recognition motifs, and revealed the role of exon 3 and 4 skipping of Msi1 pre‑mRNA in regulating cisplatin resistance under hypoxia. These observations indicate that targeting Msi1 alternative splicing could represent a valuable strategy to repress Msi1 signaling in tumors overexpressing this RNA‑binding protein.
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October-2018
Volume 16 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Ma L, Shan Y, Ma H, Elshoura I, Nafees M, Yang K and Yin W: Identification of a novel splice variant of the human musashi‑1 gene. Oncol Lett 16: 5441-5448, 2018
APA
Ma, L., Shan, Y., Ma, H., Elshoura, I., Nafees, M., Yang, K., & Yin, W. (2018). Identification of a novel splice variant of the human musashi‑1 gene. Oncology Letters, 16, 5441-5448. https://doi.org/10.3892/ol.2018.9300
MLA
Ma, L., Shan, Y., Ma, H., Elshoura, I., Nafees, M., Yang, K., Yin, W."Identification of a novel splice variant of the human musashi‑1 gene". Oncology Letters 16.4 (2018): 5441-5448.
Chicago
Ma, L., Shan, Y., Ma, H., Elshoura, I., Nafees, M., Yang, K., Yin, W."Identification of a novel splice variant of the human musashi‑1 gene". Oncology Letters 16, no. 4 (2018): 5441-5448. https://doi.org/10.3892/ol.2018.9300