Fanconi anemia in twins with neutropenia: A case report

  • Authors:
    • Wenjun Deng
    • Mingyi Zhao
    • Yingting Liu
    • Lizhi Cao
    • Minghua Yang
  • View Affiliations

  • Published online on: August 14, 2018     https://doi.org/10.3892/ol.2018.9304
  • Pages: 5325-5330
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Abstract

Fanconi anemia (FA) is a rare inherited disease caused by mutations in genes that are primarily involved in DNA damage response or repair. The disease is often characterized by congenital malformations, progressive bone marrow failure, abnormal skin pigmentation patterns and susceptibility to cancer. The present study describes a pair of 4‑year‑old male twins, both of whom had been suffering from upper respiratory tract infections for >2 years. There was no indication of discomfort including fever, coughing, bleeding or fatigue from either child when the upper respiratory tract infection disappeared. Physical examination of the twins did not reveal anything significant, and no external anomalies were observed. In order to obtain additional diagnostic evidence, next‑generation gene sequencing, chromosome breakage analysis and comet assays were performed. The results revealed double heterozygous mutations in the Fanconi Anemia Complementation Group D2 gene of the twins, therefore providing a conclusive diagnosis of FA. The case highlights how difficulties in clinical diagnosis may be overcome by including genetic screening tests into the range of diagnostic tests, which may also reveal unexpected results.

Introduction

Neutropenia is a common abnormality in childhood with an incidence of 12.36% in Beijing from July 2015 to July 2016 (1), and is most often associated with infections, partcularly respiratory tract infections, skin soft tissue infections, and inherited bone marrow failure syndromes (IBMFS) is one of the causes of neutropenia. Fanconi anemia (FA), a rare autosomal recessive condition, is a hereditary IBMFS that was first described by Guido Fanconi in 1927 (2). The mortality rate of FA in the United States and Israel is 1–5 cases for every 1 million people, with a carrier frequency of ~1 in 300 individuals (3). Although it is often characterized by congenital malformations, progressive bone marrow failure, abnormal skin pigmentation patterns and susceptibility to cancer (4), at least 25% of patients with FA have few or no abnormal symptoms that may aid in its early diagnosis (5). As a hereditary disease, at least 16 genes including Fanconi Anemia Complementation Group (FANC)A, FANCC, FANCG, FANCD1 and FANCD2 have been identified to cause FA. Among these, mutations in the FANCD1 and FANCD2 genes are the causative factor in 5% of all cases of this disease (612). The present case report describes a pair of twins whom suffered recurrent upper respiratory tract infections for >2 years prior to the study. They were identified to have FA, caused by a double heterozygous mutation of FANCD2.

Case report

The present study was approved by the Ethical Committee of Xiangya Hospital (Changsha, Hunan, China). Written informed consent was obtained from the parents of the twins.

A pair of 4-year-old male mono-twins who suffered a recurrent upper respiratory tract infection for >2 years presented to the pediatric outpatient ward of Xiangya Hospital, Central South University (Changsha, China) on September 15, 2015, following a routine blood test that had been conducted on the twins (for a recurrent upper respiratory tract infection lasting >2 years) performed on the same day and had demonstrated abnormal results (the routine blood test for the elder brother revealed a white blood cell count (WBC) of 2.6× 109/l (normal range, 4–9.5×109/l), hemoglobin (HGB) of 118 g/l (normal range, 120–175 g/l), platelet count (PLT) of 340×1012/l (normal range, 125–350×109/l), and neutrophil granulocyte count (NE) of 0.7×109/l (normal range, 1.8–6.3×109/l); the younger brother demonstrated a WBC of 2.2×109/l, HGB of 110 g/l, PLT of 269×1012/l, and NE of 0.8×109/l). There was no indication of discomfort from either child. The two patients were of normal height and weight according to age (13) and had no observable physical deformities, and physical examination did not reveal anything significant. Blood samples from the twins were tested repeatedly (>10 times) every 1 to 2 months over the past 2 years, revealing WBC of 2.2×109 to 4.3×109/l, HGB from 100 to 120 g/l, PLT from 210×1012 to 314×1012/l and NE from 0.7×109 to 1×109/l. Other laboratory test results, including those for immunoglobulins (IgG, IgA, IgM and IgE), lymphocyte subsets, virus antibodies (herpes simplex virus, respiratory syncytial virus, adenovirus, Epstein-Barr virus, coxsackievirus and cytomegalovirus), random blood glucose and endocrines (sex, thyroid and growth hormones), were not abnormal. Imaging analysis, including chest radiography, and cardiac and abdominal ultrasounds, did not reveal any areas of concern. Bone marrow cell morphology was assessed as follows: The punctured bone marrow fluid was placed at one end of a slide glass, and one end of a pushing piece with a flat edge was placed in front of the bone marrow fluid, and the bone marrow fluid was smoothly pushed forward to the other end of the slide glass, so that a thin layer of bone marrow fluid remained on the slide glass, then the slide was air-dried and fixed with methanol for 5 min at 25°C. Next, the slide was dyed with Wright-Giemsa stain (Wright stain consisted of 0.1 g Wright's dye and 60 ml methanol, and the Giemsa stain consisted of 0.5 g Giemsa's dye, 33 ml methanol and 33 ml pure glycerin, purchased from Beijing Solarbio Science & Technology Co., Ltd., Beijing, China) for 5 min at 25°C prior to observation with optical microscope (magnification, ×100) to determine the bone marrow morphology and the degree of bone marrow hyperplasia. The bone marrow cell morphology test revealed a normal result. In order to obtain a more accurate diagnosis, next-generation sequencing (NGS) was performed. Genomic DNA samples obtained from 2 ml peripheral blood were sheared by sonication. The sheared genomic DNA was then hybridized using the NimbleGen 2.0 probe sequence capture array (Roche Molecular Diagnostics, Pleasanton, CA, USA) to enrich exonic DNA (Joy Orient Translational Medicine Research Centre, Co., Ltd., Beijing, China). A 1 µg DNA library was mixed with Buffer BL and GenCap gene panel probe (MyGenostics, Inc., Medford, MA, USA), heated at 95°C for 7 min and 65°C for 2 min on a polymerase chain reaction (PCR) machine. Subsequently, 23 µl of the 65°C pre-warmed Buffer HY (MyGenostics, Inc.) was added to the mix, and the mixture was held at 65°C for 22 h for hybridization. MyOne beads (50 µl; Life Technology; Thermo Fisher Scientific, Inc., Waltham, MA, USA) were washed in 500 µl 1 X binding buffer 3 times and resuspended in 80 µl 1 X binding buffer. A total of 64 µl 2 X binding buffer was added to the hybrid mix and transferred to the tube with 80 µl MyOne beads. The mix was rotated for 1 h at room temperature on a rotator. The beads were then washed with WB1 buffer at room temperature for 15 min once and WB3 buffer at 65°C for 15 min 3 times. The bound DNA was then eluted using elution buffer (1 mol/l Tris-HCl 1 ml, and 0.5 m EDTA 0.2 ml and 100 ml distilled water). The eluted DNA was amplified for 15 cycles using the following program: 98°C for 30 sec (1 cycle); 98°C for 25 sec, 65°CC for 30 sec, 72°C for 30 sec (15 cycles); and 72°C for 5 min (1 cycle). The PCR product was purified using SPRI beads (Beckman Coulter, Inc., Brea, CA, USA) according to manufacturer's protocol. The libraries were first tested for size distribution and concentration using the Agilent Bioanalyzer 2100 with the 2100 Bioanalyzer Expert software (Agilent Technologies, Inc., Santa Clara, CA, USA). The samples were then sequenced on an Illumina Hiseq2500 with HiSeq Control software (HCS2.0; Illumina, Inc., San Diego, CA, USA) and analyzed using DNASTAR software (version 5.0; DNASTAR, Inc., Madison, WI, USA). A total of 2 parallel reactions were performed for each sample, and Protein structure prediction software (v1.1, downloaded from J. Craig Venter Institute, La Jolla, CA, USA) was used to predict the functional effect of amino acid substitutions and indels. NGS revealed double heterozygous mutations in the FCND2 gene in the two patients (Tables IIII; Fig. 1). Additional diagnostic evidence obtained by chromosome breakage analysis. Chromosome culture was performed using the standardized method of the International Atomic Energy Agency 405 technical report (14). Mitomycin C (0, 50 and 100 µg/l; Kyowa Hakko Bio Co., Ltd, Tokyo, Japan) was added after 24 h and cells were cultured for 48 h at 37°C, harvested, added a potassium chloride hypotonic (0.188%) solution for 20 min at 37°C, pre-fixed with glacial acetic acid-methanol fixed fluid (consisting of glacial acetic acid and methanol at a ratio of 1:3) for 15 min at room temperature. A total of 1–2 drops was dropped onto slides from 40–50 cm and dried naturally prior to observation using a light microscope (magnification, ×100). The number of chromosome aberration cells and the chromosome breakage number were counted under the microscope, and the chromosome aberration rate and breakage rate was analyzed. The breakage rate in 100 cells was defined as (the number of broken chromosomes/100) ×100%, aberration rate was defined as (the number of cells which appeared to exhibit broken chromosomes/100) ×100%. The chromosome aberration rate and breakage rate of the experimental group were considered to be positive if they were higher compared with that of the control group. A total of 100 mitotic images were analyzed per specimen. The results are presented in Table IV. A comet assay was also performed. Cytochalasin B (6 µg/ml, at 37°C for 28 h), used to induce cell DNA damage, and ethidium bromide were purchased from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany). A Nikon 90i fluorescence microscope was purchased from Nikon Corporation (Tokyo, Japan). The chromosome image analysis system GK-1303 was purchased from Leica Microsystems GmbH (Wetzlar, Germany) and the Sanyo MCO-20AIC CO2 incubator was purchased from SANYO Semiconductor Manufacturing Co., Ltd. (Sakata, Japan). The normal-melting-point agarose was obtained from Biowest USA (Riverside, MO, USA) and the low-melting-point agarose was from Promega Corporation (Madison, WI, USA). Tris-HCl, dimethyl sulfoxide (DMSO) and Triton X-100 were purchased from Sigma-Aldrich, Merck KGaA. Lymphocyte-separated medium (lymphoprep) was purchased from Axis Shield Diagnostics, Ltd. (Dundee, UK). The horizontal-strip electrophoresis apparatus was from Bio-Rad Laboratories, Inc. (Hercules, CA, USA). The comet slides were from Bio-comet (Institute for Surgical Research and Hospital Management, Basel, Switzerland). The digital imaging system DMS300 was purchased from Leica Microsystems GmbH.

Table I.

Mutation of FANCD2 in the patients.

Table I.

Mutation of FANCD2 in the patients.

Mutation information

GeneChromosome locationBase changeAA changeMAFPredictionDisease
FANCD2Chr3:10106532 c.2141C>T(E23)p.714, P>L0.085HarmfulFanconi anemia
Chr3:10130159p.3493>T(E35)p.1165, R>W0Harmful

[i] FANCD2, Fanconi anemia complementation group 2; AA, amino acid; MAF, minor allele frequency.

Table III.

Hereditary information of a FANCD2 allele (allele 2) of the twins.

Table III.

Hereditary information of a FANCD2 allele (allele 2) of the twins.

Family memberGeneMutation typeNA changesAA changesPrediction
TwinsFCND2Heterozygous c.3493C>T(E35)p.R1165WHarmful
FatherFCND2Heterozygous c.3493C>T(E35)p.R1165WHarmful
MotherFCND2NormalC.3493C (wild-type) Normal

[i] NA, nucleic acid; AA, amino acid; FANCD2, Fanconi anemia complementation group 2.

Table IV.

Chromosome breakage test.

Table IV.

Chromosome breakage test.

A, Number of abberant cells per 100 cells.

MMC (ng/ml)

Sample050100
Twin 10.072.0±3.690.0±1.7
Twin 20.070.5±1.887.0±3.6
Control3.0±1.018.6±2.165.9±6.9

B, Number of breakage chromosomes per 100 cells.

MMC (ng/ml)

Sample050100

Twin 10.0172.0±6.0990.0±30.8
Twin 20.0188.0±3.6964.0±15.1
Control3.0±0.619.0±1.7114.8±5.3

[i] MMC, mitomycin C.

The comet assay was performed under neutral conditions, as described by Banath et al (15), but with a slight modification; specifically, special comet slides were used rather than general slides. There are gaps in comet slides to contain the agarose. Furthermore, less agarose was used in the procedure than originally described by Banath et al yielding a thinner gel agarose to enable clearer viewing under a fluorescence microscope. First, the comet slides was coated with 100 µl normal-melting-point agarose (0.075%); then, once the first agarose layer was coagulated, a mixture of 75 µl low-melting-point agarose (0.075%) and 25 µl lymphocyte suspension was applied as the second layer. The comet slides were immersed in cold fresh lysis solution (2.5 M NaCl, 1% N-sodium lauryl sarcosinate, 30 mM Na2EDTA, 10 mM Tris, 1% Triton X-100 and 10% DMSO) for 1.5 h at 4°C. Following lysis, slides were placed in a horizontal electrophoresis tank pre-filled with cold fresh Tris-borate-EDTA (TBE, Medicago, Inc., Durham, NC, USA) buffer for 20 min at 4°C to loosen the tight double-helical structure of DNA for electrophoresis. Electrophoresis was then performed at 200 mA for 20 min in TBE buffer at room temperature. The slides were then rinsed twice with distilled water and were stained with ethidium bromide (2 µg/ml) for 10 min at 25°C. All of the above procedures were performed in the dark to avoid supernumerary DNA damage. The comet slides were viewed using a Nikon 90i fluorescence microscope (magnification, ×400) and images of 100 comets were collected for each group using a digital imaging system (DMS300; Leica Microsystems GmbH). Cells that overlapped were not counted. All of the comet images were analyzed using Comet Assay Software Pect (cat. no., CASP 1.2.3 beta 1; CASPLab, University of Wroclaw, Institute of Theoretical Physics, Wroclaw, Poland), and the percentage of DNA in the comet tail, the tail length, the tail moment and the olive tail moment were recorded to characterize the lymphocytic DNA damage. Statistical analyses were performed using one-way analysis of variance and Dunnett's test with SPSS13.0 (SPSS Inc., Chicago, IL, USA), and P<0.05 was considered to indicate a statistically significant difference. The results, presented in Table V, revealed the Tail Moment of the comet experiment for twins were higher than that of the control group, which was statistically significant and therefore considered a positive result. Therefore, on the basis of these data, the twins were definitively diagnosed with FA.

Table V.

Comet assay.

Table V.

Comet assay.

GroupHead DNA, %Tail DNA, %Tail length (pixel)Comet length (pixel)Tail momentOlive tail moment
Twin 194.395.6113.3365.110.790.51
Twin 294.035.9714.0265.950.970.66
Control98.021.988.9264.030.500.60
P-value for Twin 1<0.001<0.001<0.001<0.001<0.0010.135
P-value for Twin 2<0.001<0.001<0.001<0.001<0.0010.112

[i] All comet images were analyzed using Comet Assay Software Project (cat. no., CASP 1.2.3 beta 1; CASPLab, University of Wroclaw, Institute of Theoretical Physics, Wroclaw, Poland) (29). Statistical analyses were performed using one-way analysis of variance by SPSS 13.0 (SPSS, Inc., Chicago, IL, USA), and P<0.05 was considered to indicate a statistically significant difference.

Discussion

FA is a rare hereditary autosomal recessive disease caused by mutations in FA genes that are primarily involved in DNA damage response or repair (16). It is characterized by congenital malformations, progressive bone marrow failure, skin lesions and susceptibility to cancer (17). Early symptoms of FA include multiple malformations, including skeletal and ear deformities, renal malformation and abnormal skin pigmentation patterns, including café au lait spots and chromatosis (18). These are followed by progressive bone marrow failure, which begins to occur, on average, at 7 years of age (19), with the majority of patients eventually suffering from cancer (20). As these clinical manifestations lack specificity, it is often difficult to diagnose FA, particularly in patients who do not exhibit surface deformities or skin lesions. For this reason, tests including chromosome breakage analysis and comet assays are often performed to reach a definitive diagnosis. FA diagnosis is also confirmed by the subtyping of FA (i.e., determination of the complementation group) (21,22). At present, sequencing of the FA genes has also become an important and accurate method of diagnosing the disease.

In the present case of the twins, the physical examination, necessary laboratory and imaging tests and lack of abnormal skin pigmentation patterns gave no indication of FA. Only changes in the levels of certain blood components were observed, without abnormal bone marrow cell morphology. Therefore, it was difficult to diagnose the cause of the abnormal blood results and even to select follow-up tests. Fortunately, their symptoms were similar to those of IBMFS, thereby providing a clue for the direction to follow. Next-generation gene sequencing was then performed and revealed that the two patients exhibited double heterozygous mutations in the FANCD2 gene, confirming that each of the parents was an obligate carrier. With this information, the twins were conclusively diagnosed with FA. In addition, the chromosome breakage experiment and comet assays demonstrated positive results, verifying the diagnosis.

The FANCD2 gene, located on chromosome 3 at position 3p25.3 (including 44 exons), codes for 2 isoforms of proteins (FANCD2-S and FANCD2-L) consisting of 1,451 amino acids, with molecular masses of 155 and 162 kDa, respectively. Ubiquitination of the FANCD2-encoded protein is of crucial importance in the pathogenesis of FA, as the ubiquitinated protein normally binds with Fanconi Anemia Complementation Group I and BRCA2, DNA Repair Associated, to repair damaged DNA. In the case of the two patients, next-generation sequencing revealed a c.2141C>T mutation in one of the alleles of FANCD2, termed Allele 1, which changes the 714th amino acid of this protein from a proline to a leucine. Protein structure prediction software (23) identified this change to be harmful. In addition, by testing this same FANCD2 site in the genome of the parents of the twins, it was identified that this mutation was passed on through the mother. In the other allele of FANCD2, termed Allele 2, a c.3493C>T mutation was identified, which changes the 1,165th amino acid of this protein from an arginine to a tryptophan. Protein structure prediction software also identified this change to be harmful. Testing of this site on the genomes of the parents revealed that this mutation originated from the father. On the basis of these results, it was concluded that harmful mutations in the two alleles of FANCD2 had been passed on to the twins, and they were definitively diagnosed with FA.

In summary, the following were the characteristics of the twins: Firstly, the two boys presented with the same symptoms during the early stages and appeared to be suffering from chronic illnesses for >2 years. Secondly, the twins indicated no discomfort upon hospital presentation and no positive results from the physical examination. Thirdly, specific abnormal blood test results and a positive comet assay result were observed, and double heterozygous mutations of FANCD2 were revealed. Fourthly, there were no other diseases aside from FA that accurately matched the symptoms and disease presentation of the twins. Therefore, on the basis of these results, the twins were diagnosed with FA. Analysis of the study may cause the following to be questioned: Firstly, although the twins had abnormal routine blood test results, physical and imaging examinations revealed no malformations, and no abnormal skin pigmentation patterns were identified. The results of bone marrow morphology analysis were also identified to be normal, whereas the majority of cases of FA demonstrate all of the above symptoms. A possible explanation for this inconsistency is that the twins were in the early stage, or suffered from a mild subtype, of FA. Secondly, next-generation gene sequencing of FANCD2 does not appear to be sufficient to support the diagnosis of FA, as in the case of Allele 1. Although the protein structure prediction software predicted the change to be harmful, the minor allele frequency (MAF) for this mutation is 5.9% according to the Exome Aggregation Consortium (24). However, as the MAF for this mutation is as high as 5.9%, this means that the homozygous mutation may be as high as 0.35%, but as is not consistent with the incidence of FA, whether this type of mutation leads to FA is questionable. Our interpretation is that the present MAF value of Allele 1 is not representative of all existing data. An additional possible explanation is that these types of homozygous mutations, including that of Allele 1 in FA, result in mild cases FA or are not pathogenic, but when they exist together with a second mutation, for example a double heterozygous mutation, the symptoms become more marked. In addition, as the mutation of Allele 2 has not been identified previously for this gene, its MAF value is unknown and it may be a novel mutation. Nevertheless, based on protein structure prediction software, it was deduced that this mutation was also harmful. Unfortunately, the present study was not able to examine the protein structure and its mechanism.

It is well-known that hematopoietic stem cell transplantation (HSCT) is presently the only way to improve the hematopoietic environment and the long-term disease-free survival rate of patients with FA (25). Considering the fact that an early diagnosis of FA is difficult, and alongside the known problems of transplantation failure, graft-versus-host disease and numerous other transplantation-associated issues, there is no unified consensus on when to initiate HSCT for patients with FA (26). In a retrospective analysis of the prognosis of patients with FA who had undergone allogeneic HSCT, the European Group for Blood Marrow Transplantation identified that a patient age of >10 years with a history of >20 blood transfusions and hematopoietic system failure (absolute neutrophil count <500×109/l, platelet count <20×109/l, hemoglobin <80 g/l) would be not conducive to the early reconstruction of the hematopoietic system (27). In the present case of the twin boys, due to their mild symptoms of tolerance according to the guidelines of FA (28), no additional tests or treatments were performed. The last out-patient follow-up time was December 28th, 2017, the twins had no symptoms at that time. The final routine blood tests revealed a WBC of 3.6×109/l, HGB of 116 g/l, PLT 310×1012/l, and NE of 0.9×109/l for the elder brother, and a WBC of 3.0×1109/l, HGB of 112 g/l, PLT of 258×1012/l and NE of 0.8×109/l for the younger brother. However, follow-up will be continued, and HSCT will be a viable treatment option for these patients in the future. Next-generation gene sequencing was performed for the parents, and they were provided with complete genetic information concerning FA, which may provide support in pre-implantation genetic diagnosis for future pregnancies if the couple wants to have another child.

Finally, it is necessary to state the reason the case of the present study was described, which was to share the results obtained. Firstly, the case was very rare, as each parent was a carrier for FA. Unfortunately, the illnesses of the twins were caused as a result of their double heterozygosity; however, it was notable that neither of them exhibited any typical symptoms of FA. Secondly, one novel mutation in the FADCN2 gene that may lead to FA was identified. Thirdly, the case demonstrated that difficulties in clinical diagnosis may be overcome by including genetic screening tests into the range of available diagnostic tests, which may also reveal unexpected results.

Acknowledgements

Not applicable.

Funding

The present study was supported by the National Natural Sciences Foundation of China (grant nos. 81570154, 81100359 and 81500231).

Availability of data and materials

The datasets generated and analyzed in the present study are included in this published article.

Authors' contributions

WD and MZ performed the data analysis and wrote the manuscript. LC contributed to the conception of the study. YL contributed significantly to data analysis and manuscript preparation. MY helped perform the data analysis and conducted constructive discussions. All authors approved the final manuscript.

Ethics and consent to participate

The present study was approved by the Ethical Committee of Xiangya Hospital and written informed consent was obtained from the patient, or from the parent or guardians, as appropriate.

Consent for publication

Written informed consent was obtained for the publication of data and materials.

Competing interests

The authors declare that they have no competing interests.

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October-2018
Volume 16 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Deng W, Zhao M, Liu Y, Cao L and Yang M: Fanconi anemia in twins with neutropenia: A case report. Oncol Lett 16: 5325-5330, 2018
APA
Deng, W., Zhao, M., Liu, Y., Cao, L., & Yang, M. (2018). Fanconi anemia in twins with neutropenia: A case report. Oncology Letters, 16, 5325-5330. https://doi.org/10.3892/ol.2018.9304
MLA
Deng, W., Zhao, M., Liu, Y., Cao, L., Yang, M."Fanconi anemia in twins with neutropenia: A case report". Oncology Letters 16.4 (2018): 5325-5330.
Chicago
Deng, W., Zhao, M., Liu, Y., Cao, L., Yang, M."Fanconi anemia in twins with neutropenia: A case report". Oncology Letters 16, no. 4 (2018): 5325-5330. https://doi.org/10.3892/ol.2018.9304