Photodynamic therapy reduces the inhibitory effect of osteosarcoma cells on dendritic cells by upregulating HSP70

Zhang,Fan; Zhu,Yanjie; Fan,Guoxin; Hu,Shuo

doi:10.3892/ol.2018.9322

Photodynamic therapy reduces the inhibitory effect of osteosarcoma cells on dendritic cells by upregulating HSP70

Authors:
- Fan Zhang
- Yanjie Zhu
- Guoxin Fan
- Shuo Hu
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Affiliations: Department of Orthopedics, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, P.R. China
Published online on: August 17, 2018 https://doi.org/10.3892/ol.2018.9322
Pages: 5034-5040
Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Osteosarcoma is the most common primary bone tumor and predominantly affects children and adolescents. The prognosis for patients with osteosarcoma is poor. Therefore, the development of novel treatments for osteosarcoma is required. Photodynamic therapy (PDT) is a disease site‑specific treatment that utilizes a photosensitizing agent along with light to kill cancer cells. This agent only works following activation by certain wavelengths of light. After the agent is absorbed by the cancer cells, light is then applied to the area to be treated. The light causes the drug to react with oxygen, which produces radical and reactive oxygen species that kill the cells. However, the immune reaction that occurs following PDT remains unknown. The present study demonstrated that the necrosis of osteosarcoma cells inhibited the function of dendritic cells. However, treatment of osteosarcoma cells with PDT restored the function of dendritic cells by upregulating heat shock protein 70. Taken together, the results of the present study provided insight into the subsequent molecular reaction following PDT treatment of osteosarcoma at the molecular level.

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1	Mirabello L, Troisi RJ and Savage SA: Osteosarcoma incidence and survival rates from 1973 to 2004: Data from the surveillance, epidemiology, and end results program. Cancer. 115:1531–1543. 2009. View Article : Google Scholar : PubMed/NCBI
2	Kansara M and Thomas DM: Molecular pathogenesis of osteosarcoma. DNA Cell Biol. 26:1–18. 2007. View Article : Google Scholar : PubMed/NCBI
3	Ottaviani G and Jaffe N: The epidemiology of osteosarcoma. Cancer Treat Res. 152:3–13. 2009. View Article : Google Scholar : PubMed/NCBI
4	Miao J, Wu S, Peng Z, Tania M and Zhang C: MicroRNAs in osteosarcoma: Diagnostic and therapeutic aspects. Tumor Biol. 34:2093–2098. 2013. View Article : Google Scholar
5	Huang Z: A review of progress in clinical photodynamic therapy. Technol Cancer Res Treat. 4:283–293. 2005. View Article : Google Scholar : PubMed/NCBI
6	Gong HY, Sun MX, Hu S, Tao YY, Gao B, Li GD, Cai ZD and Yao JZ: Benzochloroporphyrin derivative induced cytotoxicity and inhibition of tumor recurrence during photodynamic therapy for osteosarcoma. Asian Pac J Cancer Prev. 14:3351–3355. 2013. View Article : Google Scholar : PubMed/NCBI
7	Zeng H, Sun M, Zhou C, Yin F, Wang Z, Hua Y and Cai Z: Hematoporphyrin monomethyl ether-mediated photodynamic therapy selectively kills sarcomas by inducing apoptosis. PloS One. 8:e777272013. View Article : Google Scholar : PubMed/NCBI
8	Castano AP, Mroz P and Hamblin MR: Photodynamic therapy and anti-tumour immunity. Nat Rev Cancer. 6:535–545. 2006. View Article : Google Scholar : PubMed/NCBI
9	Merad M: Dendritic cells: Controllers of adaptive immunity. Nat Rev Immunol. 11:1–2. 2011.
10	Schuler G, Schuler-Thurner B and Steinman RM: The use of dendritic cells in cancer immunotherapy. Curr Opin Immunol. 15:138–147. 2003. View Article : Google Scholar : PubMed/NCBI
11	Merad M, Sathe P, Helft J, Miller J and Mortha A: The dendritic cell lineage: Ontogeny and function of dendritic cells and their subsets in the steady state and the inflamed setting. Annu Rev Immunol. 31:563–604. 2013. View Article : Google Scholar : PubMed/NCBI
12	Steinman RM: Decisions about dendritic cells: Past, present, and future. Annu Rev Immunol. 30:1–22. 2012. View Article : Google Scholar : PubMed/NCBI
13	Chen R, Deng X, Wu H, Peng P, Wen B and Li F and Li F: Combined immunotherapy with dendritic cells and cytokine-induced killer cells for malignant tumors: A systematic review and meta-analysis. Int Immunopharmacol. 22:451–464. 2014. View Article : Google Scholar : PubMed/NCBI
14	Guilliams M, Ginhoux F, Jakubzick C, Naik SH, Onai N, Schraml BU, Segura E, Tussiwand R and Yona S: Dendritic cells, monocytes and macrophages: A unified nomenclature based on ontogeny. Nat Rev Immunol. 14:571–578. 2014. View Article : Google Scholar : PubMed/NCBI
15	Anguille S, Smits EL, Lion E, van Tendeloo VF and Berneman ZN: Clinical use of dendritic cells for cancer therapy. Lancet Oncol. 15:e257–e267. 2014. View Article : Google Scholar : PubMed/NCBI
16	Pizova K, Tomankova K, Daskova A, Binder S, Bajgar R and Kolarova H: Photodynamic therapy for enhancing antitumour immunity. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 156:93–102. 2012. View Article : Google Scholar : PubMed/NCBI
17	Jalili A, Makowski M, Switaj T, Nowis D, Wilczynski GM, Wilczek E, Chorazy-Massalska M, Radzikowska A, Maslinski W, Biały L, et al: Effective photoimmunotherapy of murine colon carcinoma induced by the combination of photodynamic therapy and dendritic cells. Clin Cancer Res. 10:4498–4508. 2004. View Article : Google Scholar : PubMed/NCBI
18	Saczko J, Nowak M, Skolucka N, Kulbacka J and Kotulska M: The effects of the electro-photodynamic in vitro treatment on human lung adenocarcinoma cells. Bioelectrochemistry. 79:90–94. 2010. View Article : Google Scholar : PubMed/NCBI
19	Zhang M, Tang H, Guo Z, An H, Zhu X, Song W, Guo J, Huang X, Chen T, Wang J and Cao X: Splenic stroma drives mature dendritic cells to differentiate into regulatory dendritic cells. Nat Immunol. 5:1124–1133. 2004. View Article : Google Scholar : PubMed/NCBI
20	Tang H, Guo Z, Zhang M, Wang J, Chen G and Cao X: Endothelial stroma programs hematopoietic stem cells to differentiate into regulatory dendritic cells through IL-10. Blood. 108:1189–1197. 2006. View Article : Google Scholar : PubMed/NCBI
21	Xia S, Guo Z, Xu X, Yi H, Wang Q and Cao X: Hepatic microenvironment programs hematopoietic progenitor differentiation into regulatory dendritic cells, maintaining liver tolerance. Blood. 112:3175–3185. 2008. View Article : Google Scholar : PubMed/NCBI
22	Li Q, Guo Z, Xu X, Xia S and Cao X: Pulmonary stromal cells induce the generation of regulatory DC attenuating T-cell-mediated lung inflammation. Eur J Immunol. 38:2751–2761. 2008. View Article : Google Scholar : PubMed/NCBI
23	Lutz MB, Kukutsch N, Ogilvie AL, Rössner S, Koch F, Romani N and Schuler G: An advanced culture method for generating large quantities of highly pure dendritic cells from mouse bone marrow. J Immunol Methods. 223:77–92. 1999. View Article : Google Scholar : PubMed/NCBI
24	Morrison TB, Weis JJ and Wittwer CT: Quantification of low-copy transcripts by continuous SYBR Green I monitoring during amplification. Biotechniques. 24:954–958, 960, 962. 1998.PubMed/NCBI
25	Chidlow G, Wood JP and Casson RJ: Expression of inducible heat shock proteins Hsp27 and Hsp70 in the visual pathway of rats subjected to various models of retinal ganglion cell injury. PloS One. 9:e1148382014. View Article : Google Scholar : PubMed/NCBI
26	Dorak MT: Real-time PCR. Taylor & Francis; 2007, View Article : Google Scholar
27	Fraga D, Meulia T and Fenster S: Real-time PCR. Current protocols essential laboratory techniques: 10.13. 11-10.13. 40. 2008. View Article : Google Scholar
28	Floto RA, MacAry PA, Boname JM, Mien TS, Kampmann B, Hair JR, Huey OS, Houben EN, Pieters J, Day C, et al: Dendritic cell stimulation by mycobacterial Hsp70 is mediated through CCR5. Science. 314:454–458. 2006. View Article : Google Scholar : PubMed/NCBI
29	Mac Ary PA, Javid B, Floto RA, Smith KG, Oehlmann W, Singh M and Lehner PJ: HSP70 peptide binding mutants separate antigen delivery from dendritic cell stimulation. Immunity. 20:95–106. 2004. View Article : Google Scholar : PubMed/NCBI
30	Wu Y, Wan T, Zhou X, Wang B, Yang F, Li N, Chen G, Dai S, Liu S, Zhang M and Cao X: Hsp70-like protein 1 fusion protein enhances induction of carcinoembryonic antigen-specific CD8+ CTL response by dendritic cell vaccine. Cancer Res. 65:4947–4954. 2005. View Article : Google Scholar : PubMed/NCBI
31	Liu Q, Zhang C, Sun A, Zheng Y, Wang L and Cao X: Tumor-educated CD11bhighIalow regulatory dendritic cells suppress T cell response through arginase I. J Immunol. 182:6207–6216. 2009. View Article : Google Scholar : PubMed/NCBI
32	Kansara M, Teng MW, Smyth MJ and Thomas DM: Translational biology of osteosarcoma. Nat Rev Cancer. 14:722–735. 2014. View Article : Google Scholar : PubMed/NCBI
33	Chen X, Bahrami A, Pappo A, Easton J, Dalton J, Hedlund E, Ellison D, Shurtleff S, Wu G, Wei L, et al: Recurrent somatic structural variations contribute to tumorigenesis in pediatric osteosarcoma. Cell Rep. 7:104–112. 2014. View Article : Google Scholar : PubMed/NCBI