Effects of let‑7c on the proliferation of ovarian carcinoma cells by targeted regulation of CDC25a gene expression

Zhang,Wei; Zeng,Qingru; Ban,Zhenying; Cao,Jing; Chu,Tianjiao; Lei,Dongmei; Liu,Chi; Guo,Wentao; Zeng,Xianxu

doi:10.3892/ol.2018.9327

Effects of let‑7c on the proliferation of ovarian carcinoma cells by targeted regulation of CDC25a gene expression

Authors:
- Wei Zhang
- Qingru Zeng
- Zhenying Ban
- Jing Cao
- Tianjiao Chu
- Dongmei Lei
- Chi Liu
- Wentao Guo
- Xianxu Zeng
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Affiliations: Department of Pathology, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China, Department of Ultrasound, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China, Division of Transplantation Immunology, National Research Institute for Child Health and Development, Tokyo 157‑8535, Japan, Pathogen Biology Laboratory, The Basic Medical College of Guangdong Medical University, Dongguan, Guangdong 523000, P.R. China
Published online on: August 20, 2018 https://doi.org/10.3892/ol.2018.9327
Pages: 5543-5550
Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

MicroRNAs serve a role in the development of ovarian cancer (OC). The present study investigated whether let‑7c is able to regulate the proliferation of OC cells by targeting cell division cycle 25A (CDC25a). The reverse transcription‑quantitative polymerase chain reaction was performed to detect the expression of let‑7c in OC specimens. Let‑7c agomir was transfected into OC cells, and the proliferation and apoptosis of OC cells were detected. A dual‑luciferase assay and western blotting were performed to analyze whether CDC25a was the target gene of let‑7c as well as its interaction site. The results revealed that, in OC tissue, let‑7c was downregulated when compared with normal ovarian tissue. A Cell Counting Kit‑8 (CCK8) assay, colony formation assay and flow cytometry demonstrated that increased expression of let‑7c was able to inhibit the proliferation and increase the apoptosis of OC cells. Western blotting revealed that upregulated let‑7c is able to decrease the expression of CDC25a, and a dual‑luciferase assay and a recovery assay demonstrated that let‑7c was able to regulate the expression of the 3' untranslated region of CDC25a. Therefore, the roles of let‑7c in inhibiting the proliferation and promoting the apoptosis of OC cells may be realized through the regulation of the expression of CDC25a. The results of the present study revealed that let‑7c may be a novel target in the diagnosis and treatment of OC.

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