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Article Open Access

Never‑in‑mitosis A‑related kinase 8, a novel target of von‑Hippel‑Lindau tumor suppressor protein, promotes gastric cancer cell proliferation

  • Authors:
    • Xiao‑Fei Ding
    • Jie Chen
    • Jun Zhou
    • Guang Chen
    • Ying‑Liang Wu
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacology, Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, P.R. China, Laboratory for Biological Medicine, School of Medicine, Taizhou University, Taizhou, Zhejiang 318000, P.R. China, Institute of Tumor, Taizhou University, Taizhou, Zhejiang 318000, P.R. China
    Copyright: © Ding et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5900-5906
    |
    Published online on: August 20, 2018
       https://doi.org/10.3892/ol.2018.9328
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Abstract

Previous research has revealed that the von‑Hippel‑Lindau tumor suppressor protein (pVHL) may downregulate never‑in‑mitosis A‑related kinase 8 (NEK8) via hypoxia‑inducible factor‑α (HIF‑α). The HIF‑independent functions of pVHL also serve an important role in its tumor‑suppressor action. In the present study, the association between pVHL and NEK8 was demonstrated in the human gastric cancer cell line, SGC‑7901, indicating a direct interaction of pVHL with NEK8. Subsequently, it was reported that MG‑132, a specific proteasome inhibitor, may attenuate pVHL overexpression‑induced reductions in NEK8 protein expression levels. In addition, the present study revealed that pVHL may stimulate the rapid degradation of NEK8 protein and promote its ubiquitination. The association between the expression profile of NEK8 and the survival status of patients with gastric cancer was analyzed from an online database. Kaplan‑Meier survival plots indicated that higher expression levels of NEK8 may lead to poor survival, as suggested by the transcriptomic data of 1,065 patients with gastric cancer. It was found that NEK8‑knockdown mediated by RNA interference inhibited SGC‑7901 and SNU‑1 proliferation, colony formation and migration in vitro, and tumor growth in vivo. Collectively, the present study proposed that NEK8 may be a novel target of pVHL as a ubiquitin E3 ligase, and may serve a role as a potential oncoprotein in human gastric cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Ding XF, Chen J, Zhou J, Chen G and Wu YL: Never‑in‑mitosis A‑related kinase 8, a novel target of von‑Hippel‑Lindau tumor suppressor protein, promotes gastric cancer cell proliferation. Oncol Lett 16: 5900-5906, 2018.
APA
Ding, X., Chen, J., Zhou, J., Chen, G., & Wu, Y. (2018). Never‑in‑mitosis A‑related kinase 8, a novel target of von‑Hippel‑Lindau tumor suppressor protein, promotes gastric cancer cell proliferation. Oncology Letters, 16, 5900-5906. https://doi.org/10.3892/ol.2018.9328
MLA
Ding, X., Chen, J., Zhou, J., Chen, G., Wu, Y."Never‑in‑mitosis A‑related kinase 8, a novel target of von‑Hippel‑Lindau tumor suppressor protein, promotes gastric cancer cell proliferation". Oncology Letters 16.5 (2018): 5900-5906.
Chicago
Ding, X., Chen, J., Zhou, J., Chen, G., Wu, Y."Never‑in‑mitosis A‑related kinase 8, a novel target of von‑Hippel‑Lindau tumor suppressor protein, promotes gastric cancer cell proliferation". Oncology Letters 16, no. 5 (2018): 5900-5906. https://doi.org/10.3892/ol.2018.9328
Copy and paste a formatted citation
x
Spandidos Publications style
Ding XF, Chen J, Zhou J, Chen G and Wu YL: Never‑in‑mitosis A‑related kinase 8, a novel target of von‑Hippel‑Lindau tumor suppressor protein, promotes gastric cancer cell proliferation. Oncol Lett 16: 5900-5906, 2018.
APA
Ding, X., Chen, J., Zhou, J., Chen, G., & Wu, Y. (2018). Never‑in‑mitosis A‑related kinase 8, a novel target of von‑Hippel‑Lindau tumor suppressor protein, promotes gastric cancer cell proliferation. Oncology Letters, 16, 5900-5906. https://doi.org/10.3892/ol.2018.9328
MLA
Ding, X., Chen, J., Zhou, J., Chen, G., Wu, Y."Never‑in‑mitosis A‑related kinase 8, a novel target of von‑Hippel‑Lindau tumor suppressor protein, promotes gastric cancer cell proliferation". Oncology Letters 16.5 (2018): 5900-5906.
Chicago
Ding, X., Chen, J., Zhou, J., Chen, G., Wu, Y."Never‑in‑mitosis A‑related kinase 8, a novel target of von‑Hippel‑Lindau tumor suppressor protein, promotes gastric cancer cell proliferation". Oncology Letters 16, no. 5 (2018): 5900-5906. https://doi.org/10.3892/ol.2018.9328
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