Open Access

Antitumor effect of ginsenoside Rg3 on gallbladder cancer by inducing endoplasmic reticulum stress‑mediated apoptosis in vitro and in vivo

  • Authors:
    • Keren Wu
    • Jie Huang
    • Ning Li
    • Tao Xu
    • Wenyu Cai
    • Zhipeng Ye
  • View Affiliations

  • Published online on: August 20, 2018     https://doi.org/10.3892/ol.2018.9331
  • Pages: 5687-5696
  • Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Recent studies have highlighted the importance of the endoplasmic reticulum (ER) in apoptotic processes. In the present study, the traditional herbal medicine ginsenoside Rg3 was used to treat gallbladder cancer in vitro and in vivo. The underlying signaling mechanisms were investigated using various molecular biology techniques, including flow cytometry, western blot analysis, ELISA and reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). It was indicated that Rg3 exerted pro‑apoptotic activity against the gallbladder cancer cell line GBC‑SD through the ER stress‑mediated signaling pathway. This was demonstrated by increased expression of phosphorylation of eukaryotic translation‑initiation factor 2α (eIF2α), activating transcription factor 4 (ATF4), CCAAT/enhancer‑binding protein homologous protein and lipocalin 2. In addition, eIF2α and ATF4 knockdown attenuated the pro‑apoptotic effect of Rg3 by inhibiting reactive oxygen species. Furthermore, the results of RT‑qPCR analysis indicated that long intergenic non‑protein coding RNA‑p21 was significantly upregulated following Rg3 treatment. In conclusion, the results of the present study demonstrated that Rg3 inhibited tumor growth in a GBC‑SD gallbladder cancer xenograft, by upregulating the ER stress‑mediated signaling pathway. Therefore, ER stress activation is suggested to mediate the antitumor effect of Rg3 in gallbladder cancer activity in vitro and in vivo.
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November-2018
Volume 16 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Wu K, Huang J, Li N, Xu T, Cai W and Ye Z: Antitumor effect of ginsenoside Rg3 on gallbladder cancer by inducing endoplasmic reticulum stress‑mediated apoptosis in vitro and in vivo. Oncol Lett 16: 5687-5696, 2018
APA
Wu, K., Huang, J., Li, N., Xu, T., Cai, W., & Ye, Z. (2018). Antitumor effect of ginsenoside Rg3 on gallbladder cancer by inducing endoplasmic reticulum stress‑mediated apoptosis in vitro and in vivo. Oncology Letters, 16, 5687-5696. https://doi.org/10.3892/ol.2018.9331
MLA
Wu, K., Huang, J., Li, N., Xu, T., Cai, W., Ye, Z."Antitumor effect of ginsenoside Rg3 on gallbladder cancer by inducing endoplasmic reticulum stress‑mediated apoptosis in vitro and in vivo". Oncology Letters 16.5 (2018): 5687-5696.
Chicago
Wu, K., Huang, J., Li, N., Xu, T., Cai, W., Ye, Z."Antitumor effect of ginsenoside Rg3 on gallbladder cancer by inducing endoplasmic reticulum stress‑mediated apoptosis in vitro and in vivo". Oncology Letters 16, no. 5 (2018): 5687-5696. https://doi.org/10.3892/ol.2018.9331