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Article Open Access

Doxorubicin resistance induces IL6 activation in the colon cancer cell line LS180

  • Authors:
    • Xiao‑Yun Li
    • Xiao‑Feng Liao
    • Hong‑Bo Wang
    • Jian Zhang
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Xiangyang Central Hospital, Affiliated Hospital of Hubei College of Arts and Science, Xiangyang, Hubei 441021, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5923-5929
    |
    Published online on: August 23, 2018
       https://doi.org/10.3892/ol.2018.9360
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Abstract

Despite improvements in the development of drugs for the treatment of cancer, drug resistance remains a major obstacle. In colon cancer, following an initially promising response,, patients develop drug resistance, which impacts the efficacy and halts the response of cancerous cells towards drugs. In the present study, a phosphatase and tensin homolog (PTEN) knockdown model of LS180 cells, doxorubicin‑resistant models of LS180 cells as well as doxorubicin‑resistant LS180 (PTEN) knockdown model were established. The present study demonstrated that doxorubicin resistance led to the activation of interleukin (IL)6 signalling pathway which was enhanced by knockdown of PTEN. There was also an increase in the levels of IL8 and IL2 which were further enchanced by knockdown of PTEN. Doxorubicin resistance also led to an increase in the population of cancer stem cells in LS180 and shPTEN‑treated LS180 cells. Notably, doxorubicin resistance also induced epithelial to mesenchymal transition and increased the formation of mammospheres. Furthermore, the present study also reported that IL6 receptor antibody not only decreased IL6 levels but also led to a significant decreased number of cancer stem cell like population and mammosphere formation. In conclusion, in the present study it was demonstrated that doxorubicin resistance led to activation of IL6 signalling pathway which was further elevated by the knockdown of PTEN in the colon cancer cell line LS180. Thus, inhibiting the IL6 loop may provide an alternative pathway to tackle doxorubicin resistance.
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Copy and paste a formatted citation
Spandidos Publications style
Li XY, Liao XF, Wang HB and Zhang J: Doxorubicin resistance induces IL6 activation in the colon cancer cell line LS180. Oncol Lett 16: 5923-5929, 2018.
APA
Li, X., Liao, X., Wang, H., & Zhang, J. (2018). Doxorubicin resistance induces IL6 activation in the colon cancer cell line LS180. Oncology Letters, 16, 5923-5929. https://doi.org/10.3892/ol.2018.9360
MLA
Li, X., Liao, X., Wang, H., Zhang, J."Doxorubicin resistance induces IL6 activation in the colon cancer cell line LS180". Oncology Letters 16.5 (2018): 5923-5929.
Chicago
Li, X., Liao, X., Wang, H., Zhang, J."Doxorubicin resistance induces IL6 activation in the colon cancer cell line LS180". Oncology Letters 16, no. 5 (2018): 5923-5929. https://doi.org/10.3892/ol.2018.9360
Copy and paste a formatted citation
x
Spandidos Publications style
Li XY, Liao XF, Wang HB and Zhang J: Doxorubicin resistance induces IL6 activation in the colon cancer cell line LS180. Oncol Lett 16: 5923-5929, 2018.
APA
Li, X., Liao, X., Wang, H., & Zhang, J. (2018). Doxorubicin resistance induces IL6 activation in the colon cancer cell line LS180. Oncology Letters, 16, 5923-5929. https://doi.org/10.3892/ol.2018.9360
MLA
Li, X., Liao, X., Wang, H., Zhang, J."Doxorubicin resistance induces IL6 activation in the colon cancer cell line LS180". Oncology Letters 16.5 (2018): 5923-5929.
Chicago
Li, X., Liao, X., Wang, H., Zhang, J."Doxorubicin resistance induces IL6 activation in the colon cancer cell line LS180". Oncology Letters 16, no. 5 (2018): 5923-5929. https://doi.org/10.3892/ol.2018.9360
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