AQP9 promotes astrocytoma cell invasion and motility via the AKT pathway

Lv,Yao; Huang,Qiang; Dai,Weimin; Jie,Yuanqing; Yu,Guofeng; Fan,Xiaofeng; Wu,An; Miao,Qian

doi:10.3892/ol.2018.9361

November-2018 Volume 16 Issue 5

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November-2018 Volume 16 Issue 5

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Article

AQP9 promotes astrocytoma cell invasion and motility via the AKT pathway

Authors:
- Yao Lv
- Qiang Huang
- Weimin Dai
- Yuanqing Jie
- Guofeng Yu
- Xiaofeng Fan
- An Wu
- Qian Miao
View Affiliations / Copyright

Affiliations: Department of Neurosurgery, Quzhou People's Hospital, Quzhou, Zhejiang 324000, P.R. China, Department of Oncology, Quzhou People's Hospital, Quzhou, Zhejiang 324000, P.R. China
Pages: 6059-6064
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Published online on: August 23, 2018

https://doi.org/10.3892/ol.2018.9361
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Abstract

The aquaporin (AQP) family, which includes 13 members identified in mammalian cells, is involved in cancer development and progression. AQP9 expression is upregulated in several tumor tissue types. However, the functions of AQP9 in astrocytoma remain elusive. The present study identified that AQP9 was expressed in astrocytoma cells. AQP9 expression was silenced by transfection with small interfering RNAs and increased by transfection with a plasmid containing the AQP9 gene. Using invasion and wound‑healing assays, it was revealed that the knockdown of AQP9 suppressed astrocytoma cell invasion and motility, whereas overexpression of AQP9 promoted the invasion and motility of astrocytoma cells. It was further revealed that AQP9 could induce RAC serine/threonine‑protein kinase (AKT) activation and decrease E‑cadherin expression in astrocytoma cells. Inhibition of the AKT pathway attenuated AQP9‑mediated invasion, motility and E‑cadherin expression. Taken together, the results of the present study indicated that AQP9 promoted the invasion and motility of cells via the AKT pathway. Therefore, AQP9 may represent a potential target for therapeutic use of astrocytoma.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

AQP9 promotes astrocytoma cell invasion and motility via the AKT pathway

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