AQP9 promotes astrocytoma cell invasion and motility via the AKT pathway

  • Authors:
    • Yao Lv
    • Qiang Huang
    • Weimin Dai
    • Yuanqing Jie
    • Guofeng Yu
    • Xiaofeng Fan
    • An Wu
    • Qian Miao
  • View Affiliations

  • Published online on: August 23, 2018     https://doi.org/10.3892/ol.2018.9361
  • Pages: 6059-6064
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Abstract

The aquaporin (AQP) family, which includes 13 members identified in mammalian cells, is involved in cancer development and progression. AQP9 expression is upregulated in several tumor tissue types. However, the functions of AQP9 in astrocytoma remain elusive. The present study identified that AQP9 was expressed in astrocytoma cells. AQP9 expression was silenced by transfection with small interfering RNAs and increased by transfection with a plasmid containing the AQP9 gene. Using invasion and wound‑healing assays, it was revealed that the knockdown of AQP9 suppressed astrocytoma cell invasion and motility, whereas overexpression of AQP9 promoted the invasion and motility of astrocytoma cells. It was further revealed that AQP9 could induce RAC serine/threonine‑protein kinase (AKT) activation and decrease E‑cadherin expression in astrocytoma cells. Inhibition of the AKT pathway attenuated AQP9‑mediated invasion, motility and E‑cadherin expression. Taken together, the results of the present study indicated that AQP9 promoted the invasion and motility of cells via the AKT pathway. Therefore, AQP9 may represent a potential target for therapeutic use of astrocytoma.
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November-2018
Volume 16 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Lv Y, Huang Q, Dai W, Jie Y, Yu G, Fan X, Wu A and Miao Q: AQP9 promotes astrocytoma cell invasion and motility via the AKT pathway. Oncol Lett 16: 6059-6064, 2018
APA
Lv, Y., Huang, Q., Dai, W., Jie, Y., Yu, G., Fan, X. ... Miao, Q. (2018). AQP9 promotes astrocytoma cell invasion and motility via the AKT pathway. Oncology Letters, 16, 6059-6064. https://doi.org/10.3892/ol.2018.9361
MLA
Lv, Y., Huang, Q., Dai, W., Jie, Y., Yu, G., Fan, X., Wu, A., Miao, Q."AQP9 promotes astrocytoma cell invasion and motility via the AKT pathway". Oncology Letters 16.5 (2018): 6059-6064.
Chicago
Lv, Y., Huang, Q., Dai, W., Jie, Y., Yu, G., Fan, X., Wu, A., Miao, Q."AQP9 promotes astrocytoma cell invasion and motility via the AKT pathway". Oncology Letters 16, no. 5 (2018): 6059-6064. https://doi.org/10.3892/ol.2018.9361