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DNMTs inhibitor SGI‑1027 induces apoptosis in Huh7 human hepatocellular carcinoma cells

  • Authors:
    • Ning Sun
    • Jialin Zhang
    • Chengshuo Zhang
    • Bochao Zhao
    • Ao Jiao
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary and Transplantation Surgery, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China
    Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 4.0].
  • Pages: 5799-5806
    |
    Published online on: September 4, 2018
       https://doi.org/10.3892/ol.2018.9390
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Abstract

SGI‑1027, a novel class of relatively stable, highly lipophilic quinoline‑based small‑molecule inhibitors of DNA methyltransferase enzymes (DNMTs), is able to inhibit DNMTs activity, and reactivate tumor suppressor genes. However, the potential anticancer mechanisms of SGI‑1027 on human hepatocellular carcinoma (HCC) cells are still not clearly understood. Thus, the objective of the present study was to clarify the inhibitory effect of SGI‑1027 on the cell cycle and apoptosis of the Huh7 cell line. The results revealed that treatment with SGI‑1027 resulted in a significant dose‑dependent decrease in cell viability. Flow cytometric analysis identified that a 24 h treatment of SGI‑1027 resulted in cell apoptosis, and typical apoptotic nucleic alterations were observed with fluorescence microscopy following terminal deoxynucleotidyl‑transferase‑mediated dUTP nick end labeling staining. Immunoblot analysis further demonstrated that SGI‑1027 downregulated the expression of B cell lymphoma‑2 and upregulated the expression of Bcl‑associated X protein. However, no significant alterations of the cell cycle phases were observed. Overall, it is demonstrated that SGI‑1027 causes cell apoptosis via the mitochondrial‑mediated pathway, which advances current understanding of the molecular mechanisms of SGI‑1027 in HCC management.
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Copy and paste a formatted citation
Spandidos Publications style
Sun N, Zhang J, Zhang C, Zhao B and Jiao A: DNMTs inhibitor SGI‑1027 induces apoptosis in Huh7 human hepatocellular carcinoma cells. Oncol Lett 16: 5799-5806, 2018.
APA
Sun, N., Zhang, J., Zhang, C., Zhao, B., & Jiao, A. (2018). DNMTs inhibitor SGI‑1027 induces apoptosis in Huh7 human hepatocellular carcinoma cells. Oncology Letters, 16, 5799-5806. https://doi.org/10.3892/ol.2018.9390
MLA
Sun, N., Zhang, J., Zhang, C., Zhao, B., Jiao, A."DNMTs inhibitor SGI‑1027 induces apoptosis in Huh7 human hepatocellular carcinoma cells". Oncology Letters 16.5 (2018): 5799-5806.
Chicago
Sun, N., Zhang, J., Zhang, C., Zhao, B., Jiao, A."DNMTs inhibitor SGI‑1027 induces apoptosis in Huh7 human hepatocellular carcinoma cells". Oncology Letters 16, no. 5 (2018): 5799-5806. https://doi.org/10.3892/ol.2018.9390
Copy and paste a formatted citation
x
Spandidos Publications style
Sun N, Zhang J, Zhang C, Zhao B and Jiao A: DNMTs inhibitor SGI‑1027 induces apoptosis in Huh7 human hepatocellular carcinoma cells. Oncol Lett 16: 5799-5806, 2018.
APA
Sun, N., Zhang, J., Zhang, C., Zhao, B., & Jiao, A. (2018). DNMTs inhibitor SGI‑1027 induces apoptosis in Huh7 human hepatocellular carcinoma cells. Oncology Letters, 16, 5799-5806. https://doi.org/10.3892/ol.2018.9390
MLA
Sun, N., Zhang, J., Zhang, C., Zhao, B., Jiao, A."DNMTs inhibitor SGI‑1027 induces apoptosis in Huh7 human hepatocellular carcinoma cells". Oncology Letters 16.5 (2018): 5799-5806.
Chicago
Sun, N., Zhang, J., Zhang, C., Zhao, B., Jiao, A."DNMTs inhibitor SGI‑1027 induces apoptosis in Huh7 human hepatocellular carcinoma cells". Oncology Letters 16, no. 5 (2018): 5799-5806. https://doi.org/10.3892/ol.2018.9390
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