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International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Article

Anti‑IL‑20 monoclonal antibody suppresses hepatocellular carcinoma progression

  • Authors:
    • Wen‑Zhou Ding
    • Guo‑Yong Han
    • Hui‑Han Jin
    • Chuan‑Fei Zhan
    • Yuan Ji
    • Xin‑Li Huang
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary Surgery, Nanjing Medical University Affiliated Wuxi Second Hospital, Wuxi, Jiangsu 214002, P.R. China, Key Laboratory of Living Donor Liver Transplantation, National Health and Family Planning Commission, Department of Liver Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China, Department of Hepatobiliary Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, Jiangsu 210008, P.R. China
  • Pages: 6156-6162
    |
    Published online on: September 5, 2018
       https://doi.org/10.3892/ol.2018.9402
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Abstract

Interleukin (IL)‑20 is a member of the IL‑10 family of cytokines, which has been reported to participate in autoimmune inflammatory diseases. However, the potential role of IL‑20 in hepatocellular carcinoma (HCC) progression has not yet been investigated. In the present study, it was observed that IL‑20 mRNA and protein levels were markedly increased in the HCC tissues examined via reverse transcription‑quantitative polymerase chain reaction and immunohistochemical staining. In addition, IL‑20 expression was significantly associated with tumor size, metastasis, TNM stage and poor prognosis in patients with HCC. Mouse recombinant IL‑20 (mIL‑20) enhanced liver cancer cell proliferation, migration and invasion in vitro, while the anti‑IL‑20 monoclonal antibody (mAb) attenuated the effect of mIL‑20, inhibiting cancer cell migration and invasion in vitro and suppressing cell growth in vitro and in vivo. This was detected by Cell Counting Kit‑8, colony formation, Transwell assays and a xenograft tumor nude mouse model. Western blotting revealed that IL‑20 promoted HCC progression through inducing transforming growth factor‑β and matrix metalloproteinase 9 expression and enhancing the phosphorylation of Jun N‑terminal kinase and signal transducer and activator of transcription 3. The results of the present study indicated that IL‑20 promotes HCC development. In addition, anti‑IL‑20 mAb may attenuate the effect of IL‑20 and suppress liver tumorigenesis in vitro and in vivo, indicating that anti‑IL‑20 mAbs may potentially serve as effective therapeutic agents for HCC.
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Copy and paste a formatted citation
Spandidos Publications style
Ding WZ, Han GY, Jin HH, Zhan CF, Ji Y and Huang XL: Anti‑IL‑20 monoclonal antibody suppresses hepatocellular carcinoma progression. Oncol Lett 16: 6156-6162, 2018.
APA
Ding, W., Han, G., Jin, H., Zhan, C., Ji, Y., & Huang, X. (2018). Anti‑IL‑20 monoclonal antibody suppresses hepatocellular carcinoma progression. Oncology Letters, 16, 6156-6162. https://doi.org/10.3892/ol.2018.9402
MLA
Ding, W., Han, G., Jin, H., Zhan, C., Ji, Y., Huang, X."Anti‑IL‑20 monoclonal antibody suppresses hepatocellular carcinoma progression". Oncology Letters 16.5 (2018): 6156-6162.
Chicago
Ding, W., Han, G., Jin, H., Zhan, C., Ji, Y., Huang, X."Anti‑IL‑20 monoclonal antibody suppresses hepatocellular carcinoma progression". Oncology Letters 16, no. 5 (2018): 6156-6162. https://doi.org/10.3892/ol.2018.9402
Copy and paste a formatted citation
x
Spandidos Publications style
Ding WZ, Han GY, Jin HH, Zhan CF, Ji Y and Huang XL: Anti‑IL‑20 monoclonal antibody suppresses hepatocellular carcinoma progression. Oncol Lett 16: 6156-6162, 2018.
APA
Ding, W., Han, G., Jin, H., Zhan, C., Ji, Y., & Huang, X. (2018). Anti‑IL‑20 monoclonal antibody suppresses hepatocellular carcinoma progression. Oncology Letters, 16, 6156-6162. https://doi.org/10.3892/ol.2018.9402
MLA
Ding, W., Han, G., Jin, H., Zhan, C., Ji, Y., Huang, X."Anti‑IL‑20 monoclonal antibody suppresses hepatocellular carcinoma progression". Oncology Letters 16.5 (2018): 6156-6162.
Chicago
Ding, W., Han, G., Jin, H., Zhan, C., Ji, Y., Huang, X."Anti‑IL‑20 monoclonal antibody suppresses hepatocellular carcinoma progression". Oncology Letters 16, no. 5 (2018): 6156-6162. https://doi.org/10.3892/ol.2018.9402
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