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Arsenic trioxide induces the apoptosis and decreases NF‑κB expression in lymphoma cell lines

  • Authors:
    • Lu Zhong
    • Fei Xu
    • Fangyuan Chen
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    Affiliations: Department of Hematology, Renji Hospital Affiliated to Shanghai Jiaotong University, Shanghai 200001, P.R. China, Department of Ultrasound, The Affiliated Shuhuang Hospital of University of Shanghai Chinese Medicine, Shanghai 201111, P.R. China
    Copyright: © Zhong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 6267-6274
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    Published online on: September 7, 2018
       https://doi.org/10.3892/ol.2018.9424
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Abstract

Lymphoma is a type of cancer that develops from certain immune system cells. Arsenic trioxide (ATO) has attracted wide attention owing to its antitumor activities. However, the role of ATO in tumorigenesis and progression remains to be investigated. In the present study, the antitumor function of ATO was investigated in in lymphoma Raji and Jurkat cell lines and the effect of ATO on nuclear factor (NF)‑κB expression levels. A Cell Counting kit‑8 assay was used to assess cellular proliferation and the degree of cell apoptosis was measured by flow cytometric analysis; these assays demonstrated that ATO inhibited proliferation and promoted the apoptosis of Raji and Jurkat cells in a dose‑ and time‑dependent manner. Western blot analysis revealed that ATO treatment affected the expression of apoptosis‑associated proteins by downregulating the anti‑apoptotic protein B‑cell lymphoma‑2 (Bcl‑2) and upregulating the pro‑apoptotic protein Bcl‑2‑associatedX and the degree of caspase‑3 cleavage. In addition, reverse transcription‑quantitative polymerase chain reaction and western blot analysis showed that the mRNA and protein expression levels of NF‑κB were downregulated significantly following treatment with 2 µM ATO for 24, 48 and 72 h in the two cell lines. Additionally, immunofluorescence staining indicated that NF‑κB expression diminished following ATO treatment in a time‑dependent manner. These data indicated that ATO inhibited the proliferation of lymphoma cells by inducing cell apoptosis, which may be associated with the inhibition of the NF‑κB signaling pathway. The findings of the present study may lay the foundation for developing a personalized medicine strategy using ATO via targeting of the NF‑κB signaling pathway in lymphoma.
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Copy and paste a formatted citation
Spandidos Publications style
Zhong L, Xu F and Chen F: Arsenic trioxide induces the apoptosis and decreases NF‑κB expression in lymphoma cell lines. Oncol Lett 16: 6267-6274, 2018.
APA
Zhong, L., Xu, F., & Chen, F. (2018). Arsenic trioxide induces the apoptosis and decreases NF‑κB expression in lymphoma cell lines. Oncology Letters, 16, 6267-6274. https://doi.org/10.3892/ol.2018.9424
MLA
Zhong, L., Xu, F., Chen, F."Arsenic trioxide induces the apoptosis and decreases NF‑κB expression in lymphoma cell lines". Oncology Letters 16.5 (2018): 6267-6274.
Chicago
Zhong, L., Xu, F., Chen, F."Arsenic trioxide induces the apoptosis and decreases NF‑κB expression in lymphoma cell lines". Oncology Letters 16, no. 5 (2018): 6267-6274. https://doi.org/10.3892/ol.2018.9424
Copy and paste a formatted citation
x
Spandidos Publications style
Zhong L, Xu F and Chen F: Arsenic trioxide induces the apoptosis and decreases NF‑κB expression in lymphoma cell lines. Oncol Lett 16: 6267-6274, 2018.
APA
Zhong, L., Xu, F., & Chen, F. (2018). Arsenic trioxide induces the apoptosis and decreases NF‑κB expression in lymphoma cell lines. Oncology Letters, 16, 6267-6274. https://doi.org/10.3892/ol.2018.9424
MLA
Zhong, L., Xu, F., Chen, F."Arsenic trioxide induces the apoptosis and decreases NF‑κB expression in lymphoma cell lines". Oncology Letters 16.5 (2018): 6267-6274.
Chicago
Zhong, L., Xu, F., Chen, F."Arsenic trioxide induces the apoptosis and decreases NF‑κB expression in lymphoma cell lines". Oncology Letters 16, no. 5 (2018): 6267-6274. https://doi.org/10.3892/ol.2018.9424
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