miR-34a targets BCL-2 to suppress the migration and invasion of sinonasal squamous cell carcinoma

Zhao,Yigang; Wang,Xianzhi

doi:10.3892/ol.2018.9427

miR-34a targets BCL-2 to suppress the migration and invasion of sinonasal squamous cell carcinoma

Authors:
- Yigang Zhao
- Xianzhi Wang
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Affiliations: Department of Otolaryngology, Linyi People's Hospital, Linyi, Shandong 276000, P.R. China
Published online on: September 11, 2018 https://doi.org/10.3892/ol.2018.9427
Pages: 6566-6572
Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Sinonasal squamous cell carcinomas (SN-SCC) are rare tumors with low survival rate. It was reported that miR-34a expression is low in many cancers and acted as a tumor suppressor. But the biological function of miR-34a in SN-SCC has hardly been reported. Therefore, we explored the role and underlying mechanism of miR-34a in the migration and invasion of SN-SCC. Western blot analysis and RT-PCR were carried out to examine B-cell lymphoma-2 (BCL-2) and miR-34a expression in SN-SCC. Transwell assay was performed to test the SN-SCC migratory and invasive ability. Luciferase reporter assay was carried out to verify the target of miR-34a. Results demonstrated that miR-34a expression was lower in SN-SCC tissues and cells than normal SN-SCC. Re-expression of miR-34a inhibited cell migration and invasion, while had the opposite effect on inhibition of miR-34a. We also found that BCL-2 expression was higher in SN-SCC and silencing BCL-2 curbed the development of SN-SCC. BCL-2 was found to be a target of miR-34a and negatively correlated with miR-34a expression. Furthermore, BCL-2 attenuated the miR-34a inhibitory effect on SN-SCC cell migration and invasion. In short, these data demonstrated that miR-34a inhibited SN-SCC cell migration and invasion through targeting BCL-2.

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