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Article

The SOX2OT/miR‑194‑5p axis regulates cell proliferation and mobility of gastric cancer through suppressing epithelial‑mesenchymal transition

  • Authors:
    • Ruqiong Wei
    • Can Ding
    • Raquel Alarcòn Rodrìguez
    • Marìa del Mar Requena Mullor
  • View Affiliations / Copyright

    Affiliations: Department of Nursing, Physiotherapy and Medicine, Universidad de Almería, Almería 04120, Spain
    Copyright: © Wei et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 6361-6368
    |
    Published online on: September 12, 2018
       https://doi.org/10.3892/ol.2018.9433
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Abstract

Recent studies reported that long noncoding RNAs (LncRNAs) were involved in tumorigenesis of various human cancer types, including gastric cancer (GC) through targeting microRNAs (miRNAs/miRs). The present study investigated the biological functions of LncRNA SOX2 overlapping transcript (SOX2OT)/miR‑194‑5p axis and its underlying mechanism in the tumor progression of GC. The results showed that relative expression of LncRNA SOX2OT was highly upregulated while the expression of miR‑194‑5p was down‑regulated in GC tissues and cell lines (MGC‑803, SGC‑7901, MKN‑74). Knockdown of SOX2OT inhibited cell proliferation, invasion and migration of GC cells (MGC803, MKN‑74) through reducing epithelial‑mesenchymal transition (EMT). Moreover, miR‑194‑5p was predicted to be one of the targets of SOX2OT through bioinformatics analysis and was verified by luciferase reporter assay. miR‑194‑5p expression was negatively regulated by SOX2OT expression in GC cells and miR‑194‑5p inhibitor was found to counteract the inhibitory effects of SOX2OT short hairpin (sh)RNA on cell proliferation and mobility through enhancing EMT in GC cells. Taken together, the in vitro experiments revealed that knockdown of SOX2OT inhibited cell proliferation and mobility through suppressing EMT via targeting miR‑194‑5p in GC. In addition, results from in vivo experiments showed that knockdown of SOX2OT suppressed GC tumor growth and matrix metalloproteinase (MMP)‑2 and MMP‑9 expression through inhibiting EMT. Besides that, relative expression of miR‑194‑5p was increased in sh‑SOX2OT group compared with sh‑NC group. In summary, our study elucidated that the SOX2OT/miR‑194‑5p axis participated in the tumor progression of GC through regulation of EMT both in vitro and in vivo. Hence, targeting the SOX2OT/miR‑194‑5p axis may aid in establishing novel strategies for therapy of GC.
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Copy and paste a formatted citation
Spandidos Publications style
Wei R, Ding C, Rodrìguez RA and Requena Mullor Md: The SOX2OT/miR‑194‑5p axis regulates cell proliferation and mobility of gastric cancer through suppressing epithelial‑mesenchymal transition. Oncol Lett 16: 6361-6368, 2018.
APA
Wei, R., Ding, C., Rodrìguez, R.A., & Requena Mullor, M.d. (2018). The SOX2OT/miR‑194‑5p axis regulates cell proliferation and mobility of gastric cancer through suppressing epithelial‑mesenchymal transition. Oncology Letters, 16, 6361-6368. https://doi.org/10.3892/ol.2018.9433
MLA
Wei, R., Ding, C., Rodrìguez, R. A., Requena Mullor, M. d."The SOX2OT/miR‑194‑5p axis regulates cell proliferation and mobility of gastric cancer through suppressing epithelial‑mesenchymal transition". Oncology Letters 16.5 (2018): 6361-6368.
Chicago
Wei, R., Ding, C., Rodrìguez, R. A., Requena Mullor, M. d."The SOX2OT/miR‑194‑5p axis regulates cell proliferation and mobility of gastric cancer through suppressing epithelial‑mesenchymal transition". Oncology Letters 16, no. 5 (2018): 6361-6368. https://doi.org/10.3892/ol.2018.9433
Copy and paste a formatted citation
x
Spandidos Publications style
Wei R, Ding C, Rodrìguez RA and Requena Mullor Md: The SOX2OT/miR‑194‑5p axis regulates cell proliferation and mobility of gastric cancer through suppressing epithelial‑mesenchymal transition. Oncol Lett 16: 6361-6368, 2018.
APA
Wei, R., Ding, C., Rodrìguez, R.A., & Requena Mullor, M.d. (2018). The SOX2OT/miR‑194‑5p axis regulates cell proliferation and mobility of gastric cancer through suppressing epithelial‑mesenchymal transition. Oncology Letters, 16, 6361-6368. https://doi.org/10.3892/ol.2018.9433
MLA
Wei, R., Ding, C., Rodrìguez, R. A., Requena Mullor, M. d."The SOX2OT/miR‑194‑5p axis regulates cell proliferation and mobility of gastric cancer through suppressing epithelial‑mesenchymal transition". Oncology Letters 16.5 (2018): 6361-6368.
Chicago
Wei, R., Ding, C., Rodrìguez, R. A., Requena Mullor, M. d."The SOX2OT/miR‑194‑5p axis regulates cell proliferation and mobility of gastric cancer through suppressing epithelial‑mesenchymal transition". Oncology Letters 16, no. 5 (2018): 6361-6368. https://doi.org/10.3892/ol.2018.9433
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