MicroRNA‑143 regulates cell migration and invasion by targeting GOLM1 in cervical cancer

Zhou,Meiying; Chen,Xiaohong; Wu,Jian; He,Xiaoyan; Ren,Rui

doi:10.3892/ol.2018.9441

MicroRNA‑143 regulates cell migration and invasion by targeting GOLM1 in cervical cancer

Authors:
- Meiying Zhou
- Xiaohong Chen
- Jian Wu
- Xiaoyan He
- Rui Ren
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Affiliations: Department of Gynecology, Gansu Provincial Hospital, Lanzhou, Gansu 730000, P.R. China, Department of Burn, Gansu Provincial Hospital, Lanzhou, Gansu 730000, P.R. China
Published online on: September 17, 2018 https://doi.org/10.3892/ol.2018.9441
Pages: 6393-6400
Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Accumulated research has revealed that the abnormal expression of microRNAs play a crucial role in tumorigenesis, potentially serving as therapeutic biomarkers in multiple tumors including cervical cancer. However, the expression level, biological role and the underlying mechanism of miRNA‑143 in cervical cancer remain unclear. In the current study, we analyzed the miRNA‑143 and golgi membrane protein 1 (GOLM1) expression in cervical cancer tissues and cells to explore their effects on cervical cancer occurrence and metastasis. Reverse transcription-quantitative PCR (RT‑qPCR) was used to detect the miRNA‑143 expression in cervical cancer tissues and cells. Following transfection, cell Transwell assays, western blot analysis and luciferase reporter assays were carried out in human cervical cancer cells. Results demonstrated that the miRNA‑143 expression was dramatically decreased in both cervical cancer tissue samples and cells in contrast with the control group. We also found that the miRNA‑143 expression negatively correlated with the GOLM1 expression in cervical cancer tissues and miRNA‑143 inhibited cell invasion and migration via targeting GOLM1 in cervical cancer.

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