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Serum levels of candidate microRNA diagnostic markers differ among the stages of non‑small‑cell lung cancer

  • Authors:
    • Toshiko Aiso
    • Kouki Ohtsuka
    • Makiko Ueda
    • Shin Karita
    • Takuma Yokoyama
    • Saori Takata
    • Naoko Matsuki
    • Haruhiko Kondo
    • Hajime Takizawa
    • Annabelle A. Okada
    • Takashi Watanabe
    • Hiroaki Ohnishi
  • View Affiliations / Copyright

    Affiliations: Department of Medical Technology, Faculty of Health Sciences, Kyorin University, Tokyo 181‑8612, Japan, Department of Laboratory Medicine, School of Medicine, Kyorin University, Tokyo 181‑8611, Japan, Department of General Thoracic Surgery, School of Medicine, Kyorin University, Tokyo 181‑8611, Japan, Department of Respiratory Medicine, School of Medicine, Kyorin University, Tokyo 181‑8611, Japan, Department of Ophthalmology, School of Medicine, Kyorin University, Tokyo 181‑8611, Japan
    Copyright: © Aiso et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 6643-6651
    |
    Published online on: September 20, 2018
       https://doi.org/10.3892/ol.2018.9464
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Abstract

Circulating microRNAs (miRNAs) are promising markers for cancer diagnosis and prognosis. Numerous studies evaluating miRNAs as markers for non‑small cell lung cancer (NSCLC) have been conducted in recent years; however, the majority of candidate markers proposed via individual studies were inconsistent and no marker miRNAs for the diagnosis of early stage NSCLC have been established. In the present study, miR‑145, miR‑20a, miR‑21 and miR‑223, which were previously reported as candidate diagnostic markers of NSCLC, were re‑evaluated. The serum levels of these miRNAs were quantified in 56 patients with stage I‑IV NSCLC using the TaqMan microRNA assays and separately compared the levels at each stage with those in 26 control patients. The level of miR‑145 was significantly reduced in patients with NSCLC, regardless of clinical stage, and its level increased following tumor resection in patients with stage I‑II disease. These results indicate that miR‑145 is relevant as a diagnostic marker for stages I‑IV NSCLC. Additionally, the levels of miR‑20a and miR‑21 demonstrated notable differences among patients at different clinical stages. These miRNAs distinguished patients in a number of, but not all, stages of NSCLC from cancer‑free control patients. These results indicated that it is essential to analyze miRNA levels at each stage separately in order to evaluate marker miRNAs for NSCLC diagnosis.
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Copy and paste a formatted citation
Spandidos Publications style
Aiso T, Ohtsuka K, Ueda M, Karita S, Yokoyama T, Takata S, Matsuki N, Kondo H, Takizawa H, Okada AA, Okada AA, et al: Serum levels of candidate microRNA diagnostic markers differ among the stages of non‑small‑cell lung cancer. Oncol Lett 16: 6643-6651, 2018.
APA
Aiso, T., Ohtsuka, K., Ueda, M., Karita, S., Yokoyama, T., Takata, S. ... Ohnishi, H. (2018). Serum levels of candidate microRNA diagnostic markers differ among the stages of non‑small‑cell lung cancer. Oncology Letters, 16, 6643-6651. https://doi.org/10.3892/ol.2018.9464
MLA
Aiso, T., Ohtsuka, K., Ueda, M., Karita, S., Yokoyama, T., Takata, S., Matsuki, N., Kondo, H., Takizawa, H., Okada, A. A., Watanabe, T., Ohnishi, H."Serum levels of candidate microRNA diagnostic markers differ among the stages of non‑small‑cell lung cancer". Oncology Letters 16.5 (2018): 6643-6651.
Chicago
Aiso, T., Ohtsuka, K., Ueda, M., Karita, S., Yokoyama, T., Takata, S., Matsuki, N., Kondo, H., Takizawa, H., Okada, A. A., Watanabe, T., Ohnishi, H."Serum levels of candidate microRNA diagnostic markers differ among the stages of non‑small‑cell lung cancer". Oncology Letters 16, no. 5 (2018): 6643-6651. https://doi.org/10.3892/ol.2018.9464
Copy and paste a formatted citation
x
Spandidos Publications style
Aiso T, Ohtsuka K, Ueda M, Karita S, Yokoyama T, Takata S, Matsuki N, Kondo H, Takizawa H, Okada AA, Okada AA, et al: Serum levels of candidate microRNA diagnostic markers differ among the stages of non‑small‑cell lung cancer. Oncol Lett 16: 6643-6651, 2018.
APA
Aiso, T., Ohtsuka, K., Ueda, M., Karita, S., Yokoyama, T., Takata, S. ... Ohnishi, H. (2018). Serum levels of candidate microRNA diagnostic markers differ among the stages of non‑small‑cell lung cancer. Oncology Letters, 16, 6643-6651. https://doi.org/10.3892/ol.2018.9464
MLA
Aiso, T., Ohtsuka, K., Ueda, M., Karita, S., Yokoyama, T., Takata, S., Matsuki, N., Kondo, H., Takizawa, H., Okada, A. A., Watanabe, T., Ohnishi, H."Serum levels of candidate microRNA diagnostic markers differ among the stages of non‑small‑cell lung cancer". Oncology Letters 16.5 (2018): 6643-6651.
Chicago
Aiso, T., Ohtsuka, K., Ueda, M., Karita, S., Yokoyama, T., Takata, S., Matsuki, N., Kondo, H., Takizawa, H., Okada, A. A., Watanabe, T., Ohnishi, H."Serum levels of candidate microRNA diagnostic markers differ among the stages of non‑small‑cell lung cancer". Oncology Letters 16, no. 5 (2018): 6643-6651. https://doi.org/10.3892/ol.2018.9464
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