Lipid rafts regulate the lamellipodia formation of melanoma A375 cells via actin cytoskeleton‑mediated recruitment of β1 and β3 integrin

Bi,Jiajia; Wang,Ruifei; Zeng,Xianlu

doi:10.3892/ol.2018.9466

Lipid rafts regulate the lamellipodia formation of melanoma A375 cells via actin cytoskeleton‑mediated recruitment of β1 and β3 integrin

Authors:
- Jiajia Bi
- Ruifei Wang
- Xianlu Zeng
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Affiliations: Synthetic Biology Engineering Lab of Henan Province, School of Life Sciences and Technology, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China, Key Laboratory for Microorganisms and Functional Molecules, College of Life Sciences, Henan Normal University, Xinxiang, Henan 453007, P.R. China, Institute of Genetics and Cytology, School of Life Sciences, Northeast Normal University, Changchun, Jilin 130024, P.R. China
Published online on: September 20, 2018 https://doi.org/10.3892/ol.2018.9466
Pages: 6540-6546
Copyright: © Bi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Lipid rafts, distinct liquid‑ordered plasma membrane microdomains, have been shown to regulate tumor cell migration by internalizing and recycling cell‑surface proteins. The present study reports that lipid rafts are a prerequisite for lamellipodia formation, which is the first step in the processes of tumor cell migration. The results from the wound‑healing assay and immunostaining indicated that lipid rafts were asymmetrically distributed to the leading edge of migrating melanoma A375 cells during lamellipodia formation. When the integrity of lipids rafts was disrupted, lamellipodia formation was inhibited. The investigation of possible molecular mechanisms indicated that lipid rafts recruited β1 and β3 integrins, two important adhesion proteins for cell migration, to the lamellipodia. However, the different distribution characteristics of β1 and β3 integrins implied disparate functions in lamellipodia formation. Further immunostaining experiments showed that the actin cytoskeleton was responsible for lipid raft‑mediated β1 and β3 integrin distribution in the lamellipodia. Together, these findings provide novel insights into the regulation of lipid rafts in lamellipodia formation, and suggest that lipid rafts may be novel and attractive targets for cancer therapy.

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