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Article

Utilizing ethacrynic acid and ciclopirox olamine in liver cancer

  • Authors:
    • Ahmad M. Al‑Dali
    • Hans Weiher
    • Ingo G.H. Schmidt‑Wolf
  • View Affiliations / Copyright

    Affiliations: Center for Integrated Oncology, University Hospital Bonn, Bonn D‑53105, Germany, Department of Immunology and Cell Biology, Bonn‑Rhein‑Sieg University of Applied Sciences, Rheinbach D‑53359, Germany
  • Pages: 6854-6860
    |
    Published online on: September 20, 2018
       https://doi.org/10.3892/ol.2018.9472
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Abstract

Once aberrantly activated, the Wnt/β‑catenin pathway may result in uncontrolled proliferation and eventually cancer. Efforts to counter and inhibit this pathway are mainly directed against β‑catenin, as it serves a role on the cytoplasm and the nucleus. In addition, specially‑generated lymphocytes are recruited for the purpose of treating liver cancer. Peripheral blood mononuclear lymphocytes are expanded by the timely addition of interferon γ, interleukin (IL)‑1β, IL‑2 and anti‑cluster of differentiation 3 antibody. The resulting cells are called cytokine‑induced killer (CIK) cells. The present study utilised these cells and combine them with drugs inhibiting the Wnt pathway in order to examine whether this resulted in an improvement in the killing ability of CIK cells against liver cancer cells. Drugs including ethacrynic acid (EA) and ciclopirox olamine (CPX) were determined to be suitable candidates, as determined by previous studies. Drugs were administered on their own and combined with CIK cells and then a cell viability assay was performed. These results suggest that EA‑treated cells demonstrated apoptosis and were significantly affected compared with untreated cells. Unlike EA, CPX killed normal and cancerous cells even at low concentrations. Subsequent to combining EA with CIK cells, the potency of killing was increased and a greater number of cells died, which proves a synergistic action. In summary, EA may be used as an anti‑hepatocellular carcinoma drug, while CPX possesses a high toxicity to cancerous as well as to normal cells. It was proposed that EA should be integrated into present therapeutic methods for cancer.
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1 

Forner A, Llovet JM and Bruix J: Hepatocellular carcinoma. Lancet. 379:1245–1255. 2012. View Article : Google Scholar : PubMed/NCBI

2 

Llovet JM, Burroughs A and Bruix J: Hepatocellular carcinoma. Lancet. 362:1907–1917. 2003. View Article : Google Scholar : PubMed/NCBI

3 

Venook AP, Papandreou C, Furuse J and de Guevara LL: The incidence and epidemiology of hepatocellular carcinoma: A global and regional perspective. Oncologist. 15 Suppl 4:S5–S13. 2010. View Article : Google Scholar

4 

Bialecki ES and Di Bisceglie AM: Diagnosis of hepatocellular carcinoma. HPB Oxf. 7:26–34. 2005. View Article : Google Scholar

5 

Lin S, Hoffmann K and Schemmer P: Treatment of hepatocellular carcinoma: A systematic review. Liver Cancer. 1:144–158. 2012. View Article : Google Scholar : PubMed/NCBI

6 

Tang B, Tang F, Wang Z, Qi G, Liang X, Li B, Yuan S, Liu J, Yu S and He S: Overexpression of CTNND1 in hepatocellular carcinoma promotes carcinous characters through activation of Wnt/β-catenin signaling. J Exp Clin Cancer Res. 35:822016. View Article : Google Scholar : PubMed/NCBI

7 

Clevers H: Wnt/beta-catenin signaling in development and disease. Cell. 127:469–480. 2006. View Article : Google Scholar : PubMed/NCBI

8 

Lu D, Liu JX, Endo T, Zhou H, Yao S, Willert K, Schmidt-Wolf IG, Kipps TJ and Carson DA: Ethacrynic acid exhibits selective toxicity to chronic lymphocytic leukemia cells by inhibition of the Wnt/beta-catenin pathway. PLoS One. 4:e82942009. View Article : Google Scholar : PubMed/NCBI

9 

Zhou H, Shen T, Luo Y, Liu L, Chen W, Xu B, Han X, Pang J, Rivera CA and Huang S: The antitumor activity of the fungicide ciclopirox. Int J Cancer. 127:2467–2477. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Schmidt-Wolf IG, Negrin RS, Kiem HP, Blume KG and Weissman IL: Use of a SCID mouse/human lymphoma model to evaluate cytokine-induced killer cells with potent antitumor cell activity. J Exp Med. 174:139–149. 1991. View Article : Google Scholar : PubMed/NCBI

11 

Sangiolo D: Cytokine induced killer cells as promising immunotherapy for solid tumors. J Cancer. 2:363–368. 2011. View Article : Google Scholar : PubMed/NCBI

12 

Technologies DM: Inc., . Cell Counting Kit-8. Jan 24–2017http://www.dojindo.com/store/p/456-Cell-Counting-Kit-8.html

13 

Schmidt M, Kim Y, Gast SM, Endo T, Lu D, Carson D and Schmidt-Wolf IG: Increased in vivo efficacy of lenalidomide and thalidomide by addition of ethacrynic acid. In Vivo. 25:325–334. 2011.PubMed/NCBI

14 

Vilchez V, Turcios L, Marti F and Gedaly R: Targeting Wnt/β-catenin pathway in hepatocellular carcinoma treatment. World J Gastroenterol. 22:823–832. 2016. View Article : Google Scholar : PubMed/NCBI

15 

Cieply B, Zeng G, Proverbs-Singh T, Geller DA and Monga SP: Unique phenotype of hepatocellular cancers with exon-3 mutations in beta-catenin gene. Hepatology. 49:821–831. 2009. View Article : Google Scholar : PubMed/NCBI

16 

Von S, chulz-Hausmann SA, Schmeel LC, Schmeel FC and Schmidt-Wolf IG: Targeting the Wnt/beta-catenin pathway in renal cell carcinoma. Anticancer Res. 34:4101–4108. 2014.PubMed/NCBI

17 

Schmeel LC, Schmeel FC, Kim Y, Endo T, Lu D and Schmidt-Wolf IG: Targeting the Wnt/beta-catenin pathway in multiple myeloma. Anticancer Res. 33:4719–4726. 2013.PubMed/NCBI

18 

Weir SJ, Patton L, Castle K, Rajewski L, Kasper J and Schimmer AD: The repositioning of the anti-fungal agent ciclopirox olamine as a novel therapeutic agent for the treatment of haematologic malignancy. J Clin Pharm Ther. 36:128–134. 2011. View Article : Google Scholar : PubMed/NCBI

19 

Lu D, Zhao Y, Tawatao R, Cottam HB, Sen M, Leoni LM, Kipps TJ, Corr M and Carson DA: Activation of the Wnt signaling pathway in chronic lymphocytic leukemia. Proc Natl Acad Sci USA. 101:3118–3123. 2004. View Article : Google Scholar : PubMed/NCBI

20 

Kim Y, Alpmann P, Blaum-Feder S, Krämer S, Endo T, Lu D, Carson D and Schmidt-Wolf IG: Increased in vivo efficacy of lenalidomide by addition of piroctone olamine. In Vivo. 25:99–103. 2011.PubMed/NCBI

21 

Kim Y, Alpmann P, Blaum-Feder S, Krämer S, Endo T, Lu D, Carson D and Schmidt-Wolf IG: In vivo efficacy of griseofulvin against multiple myeloma. Leuk Res. 35:1070–1073. 2011. View Article : Google Scholar : PubMed/NCBI

22 

Guo Y and Han W: Cytokine-induced killer (CIK) cells: From basic research to clinical translation. Chin J Cancer. 34:99–107. 2015. View Article : Google Scholar : PubMed/NCBI

23 

Song S, Christova T, Perusini S, Alizadeh S, Bao RY, Miller BW, Hurren R, Jitkova Y, Gronda M, Isaac M, et al: Wnt inhibitor screen reveals iron dependence of β-catenin signaling in cancers. Cancer Res. 71:7628–7639. 2011. View Article : Google Scholar : PubMed/NCBI

24 

Jiang X, Hao HX, Growney JD, Woolfenden S, Bottiglio C, Ng N, Lu B, Hsieh MH, Bagdasarian L, Meyer R, et al: Inactivating mutations of RNF43 confer Wnt dependency in pancreatic ductal adenocarcinoma. Proc Natl Acad Sci USA. 110:12649–12654. 2013. View Article : Google Scholar : PubMed/NCBI

25 

Bar-Yehuda S, Stemmer SM, Madi L, Castel D, Ochaion A, Cohen S, Barer F, Zabutti A, Perez-Liz G, Del Valle L, et al: The A3 adenosine receptor agonist CF102 induces apoptosis of hepatocellular carcinoma via de-regulation of the Wnt and NF-kappaB signal transduction pathways. Int J Oncol. 33:287–295. 2008.PubMed/NCBI

26 

Stemmer SM, Benjaminov O, Medalia G, Ciuraru NB, Silverman MH, Bar-Yehuda S, Fishman S, Harpaz Z, Farbstein M, Cohen S, et al: CF102 for the treatment of hepatocellular carcinoma: A phase I/II, open-label, dose-escalation study. Oncologist. 18:25–26. 2013. View Article : Google Scholar : PubMed/NCBI

27 

López-Terrada D, Cheung SW, Finegold MJ and Knowles BB: Hep G2 is a hepatoblastoma-derived cell line. Human Pathol. 40:1512–1515. 2009. View Article : Google Scholar

28 

Buendia MA: Genetic alterations in hepatoblastoma and hepatocellular carcinoma: Common and distinctive aspects. Med Pediatr Oncol. 39:530–535. 2002. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Al‑Dali AM, Weiher H and Schmidt‑Wolf IG: Utilizing ethacrynic acid and ciclopirox olamine in liver cancer. Oncol Lett 16: 6854-6860, 2018.
APA
Al‑Dali, A.M., Weiher, H., & Schmidt‑Wolf, I.G. (2018). Utilizing ethacrynic acid and ciclopirox olamine in liver cancer. Oncology Letters, 16, 6854-6860. https://doi.org/10.3892/ol.2018.9472
MLA
Al‑Dali, A. M., Weiher, H., Schmidt‑Wolf, I. G."Utilizing ethacrynic acid and ciclopirox olamine in liver cancer". Oncology Letters 16.5 (2018): 6854-6860.
Chicago
Al‑Dali, A. M., Weiher, H., Schmidt‑Wolf, I. G."Utilizing ethacrynic acid and ciclopirox olamine in liver cancer". Oncology Letters 16, no. 5 (2018): 6854-6860. https://doi.org/10.3892/ol.2018.9472
Copy and paste a formatted citation
x
Spandidos Publications style
Al‑Dali AM, Weiher H and Schmidt‑Wolf IG: Utilizing ethacrynic acid and ciclopirox olamine in liver cancer. Oncol Lett 16: 6854-6860, 2018.
APA
Al‑Dali, A.M., Weiher, H., & Schmidt‑Wolf, I.G. (2018). Utilizing ethacrynic acid and ciclopirox olamine in liver cancer. Oncology Letters, 16, 6854-6860. https://doi.org/10.3892/ol.2018.9472
MLA
Al‑Dali, A. M., Weiher, H., Schmidt‑Wolf, I. G."Utilizing ethacrynic acid and ciclopirox olamine in liver cancer". Oncology Letters 16.5 (2018): 6854-6860.
Chicago
Al‑Dali, A. M., Weiher, H., Schmidt‑Wolf, I. G."Utilizing ethacrynic acid and ciclopirox olamine in liver cancer". Oncology Letters 16, no. 5 (2018): 6854-6860. https://doi.org/10.3892/ol.2018.9472
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