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Article Open Access

Ganoderic acid A exerts antitumor activity against MDA‑MB‑231 human breast cancer cells by inhibiting the Janus kinase 2/signal transducer and activator of transcription 3 signaling pathway

  • Authors:
    • Yuguang Yang
    • Hongfeng Zhou
    • Wenming Liu
    • Jin Wu
    • Xiaolong Yue
    • Jincai Wang
    • Lina Quan
    • Hang Liu
    • Li Guo
    • Zhipeng Wang
    • Xin Lian
    • Qingyuan Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Medical Oncology, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang 150081, P.R. China, Department of Medical Oncology, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China, Department of Medical Oncology, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China
    Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 6515-6521
    |
    Published online on: September 21, 2018
       https://doi.org/10.3892/ol.2018.9475
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Abstract

Breast cancer is a common malignant tumor among females, with triple‑negative breast cancer being an important type accounting for 15‑20% of all breast cancer cases. Triple‑negative breast cancer is one of the most aggressive types of cancer without standard adjuvant chemotherapy. Ganoderic acid A (GA‑A) is one of the major bioactive Ganoderma triterpenoids isolated from Ganoderma, which are recognized for their preventative and therapeutic effects. In the present study, the antineoplastic effect of GA‑A on human breast cancer was investigated and the pro‑apoptotic function of Janus kinase (JAK)2 and signal transducer and activator of transcription (STAT)3 on the function of GA‑A was revealed. GA‑A treatment inhibited the invasion of MDA‑MB‑231 cells. In addition, GA‑A exhibited significant antitumor activity by enhancing the apoptotic index and reactive oxygen species production. In the present study, GA‑A was identified to directly inhibit JAK2 phosphorylation and STAT3 downstream activation. In addition, GA‑A suppressed STAT3 target gene expression, including B cell lymphoma‑extra‑large and Myeloid cell leukemia 1, resulting in elevated levels of proteins associated with mitochondrial apoptosis in addition to inhibitors of cyclin‑dependent kinase. GA‑A, in combination with AG490, a JAK2/STAT3 inhibitor, further decreased MDA‑MB‑231 cell viability. In conclusion, GA‑A treatment inhibited breast cancer cell viability via JAK2/STAT3 downregulation and may regulate associated targets to serve an anti‑MDA‑MB‑231 role, including mitochondrial apoptosis and regulating the expression of cell‑cycle‑associated factors.
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Copy and paste a formatted citation
Spandidos Publications style
Yang Y, Zhou H, Liu W, Wu J, Yue X, Wang J, Quan L, Liu H, Guo L, Wang Z, Wang Z, et al: Ganoderic acid A exerts antitumor activity against MDA‑MB‑231 human breast cancer cells by inhibiting the Janus kinase 2/signal transducer and activator of transcription 3 signaling pathway. Oncol Lett 16: 6515-6521, 2018.
APA
Yang, Y., Zhou, H., Liu, W., Wu, J., Yue, X., Wang, J. ... Zhang, Q. (2018). Ganoderic acid A exerts antitumor activity against MDA‑MB‑231 human breast cancer cells by inhibiting the Janus kinase 2/signal transducer and activator of transcription 3 signaling pathway. Oncology Letters, 16, 6515-6521. https://doi.org/10.3892/ol.2018.9475
MLA
Yang, Y., Zhou, H., Liu, W., Wu, J., Yue, X., Wang, J., Quan, L., Liu, H., Guo, L., Wang, Z., Lian, X., Zhang, Q."Ganoderic acid A exerts antitumor activity against MDA‑MB‑231 human breast cancer cells by inhibiting the Janus kinase 2/signal transducer and activator of transcription 3 signaling pathway". Oncology Letters 16.5 (2018): 6515-6521.
Chicago
Yang, Y., Zhou, H., Liu, W., Wu, J., Yue, X., Wang, J., Quan, L., Liu, H., Guo, L., Wang, Z., Lian, X., Zhang, Q."Ganoderic acid A exerts antitumor activity against MDA‑MB‑231 human breast cancer cells by inhibiting the Janus kinase 2/signal transducer and activator of transcription 3 signaling pathway". Oncology Letters 16, no. 5 (2018): 6515-6521. https://doi.org/10.3892/ol.2018.9475
Copy and paste a formatted citation
x
Spandidos Publications style
Yang Y, Zhou H, Liu W, Wu J, Yue X, Wang J, Quan L, Liu H, Guo L, Wang Z, Wang Z, et al: Ganoderic acid A exerts antitumor activity against MDA‑MB‑231 human breast cancer cells by inhibiting the Janus kinase 2/signal transducer and activator of transcription 3 signaling pathway. Oncol Lett 16: 6515-6521, 2018.
APA
Yang, Y., Zhou, H., Liu, W., Wu, J., Yue, X., Wang, J. ... Zhang, Q. (2018). Ganoderic acid A exerts antitumor activity against MDA‑MB‑231 human breast cancer cells by inhibiting the Janus kinase 2/signal transducer and activator of transcription 3 signaling pathway. Oncology Letters, 16, 6515-6521. https://doi.org/10.3892/ol.2018.9475
MLA
Yang, Y., Zhou, H., Liu, W., Wu, J., Yue, X., Wang, J., Quan, L., Liu, H., Guo, L., Wang, Z., Lian, X., Zhang, Q."Ganoderic acid A exerts antitumor activity against MDA‑MB‑231 human breast cancer cells by inhibiting the Janus kinase 2/signal transducer and activator of transcription 3 signaling pathway". Oncology Letters 16.5 (2018): 6515-6521.
Chicago
Yang, Y., Zhou, H., Liu, W., Wu, J., Yue, X., Wang, J., Quan, L., Liu, H., Guo, L., Wang, Z., Lian, X., Zhang, Q."Ganoderic acid A exerts antitumor activity against MDA‑MB‑231 human breast cancer cells by inhibiting the Janus kinase 2/signal transducer and activator of transcription 3 signaling pathway". Oncology Letters 16, no. 5 (2018): 6515-6521. https://doi.org/10.3892/ol.2018.9475
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