The development of simultaneous measurement of viral load and physical status for human papillomavirus 16 and 18 co‑infection using multiplex quantitative polymerase chain reaction

Prasongdee,Prinya; Tippayawat,Patcharaporn; Limpaiboon,Temduang; Leelayuwat,Chanvit; Wongwattanakul,Molin; Jearanaikoon,Patcharee

doi:10.3892/ol.2018.9549

The development of simultaneous measurement of viral load and physical status for human papillomavirus 16 and 18 co‑infection using multiplex quantitative polymerase chain reaction

Authors:
- Prinya Prasongdee
- Patcharaporn Tippayawat
- Temduang Limpaiboon
- Chanvit Leelayuwat
- Molin Wongwattanakul
- Patcharee Jearanaikoon
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Affiliations: Center for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand
Published online on: October 4, 2018 https://doi.org/10.3892/ol.2018.9549
Pages: 6977-6987
Copyright: © Prasongdee et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Persistent infection with human papillomavirus (HPV) type 16 and 18 is known to be a major risk factor for cervical cancer. Increased prevalence of co‑infection with these high‑risk types has been observed in pre‑cancerous and cancerous tissues. The determination of physical status and copy numbers of viruses is therefore useful in clinical settings. A simple multiplex quantitative polymerase chain reaction (qPCR) for HPV16/HPV18 co‑infection in one tube reaction was established in the present study using TaqMan®‑based PCR for E2 and E6 viral DNA. The detection range was up to 106 copies with 100% specificity and high precision (CV of cycle time <0.5%). The analytical accuracy and robustness were verified by competitive assay using an unequal mixture of HPV16/HPV18 DNA. No significant effect was demonstrated when compared with the simplex qPCR. The detection of physical status was evaluated in cervical samples, including 5 pre‑cancerous and 15 cancerous samples. No significant difference was observed between simplex and multiplex qPCR (P=0.372). In conclusion, the developed multiplex qPCR method successfully demonstrated the viral status of the common HPV types in one tube. This assay will facilitate viral assessment and monitoring of cervical cancer associated with HPV16 and HPV18 co‑infection.

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