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Article

miR‑30a regulates the proliferation and invasion of breast cancer cells by targeting Snail

  • Authors:
    • Baoqiang Xiao
    • Xuejing Shi
    • Jianping Bai
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, No. 254 Hospital of The People's Liberation Army, Tianjin 300142, P.R. China, Department of Breast Surgery, Tianjin Central Obstetrics and Gynecology Hospital, Tianjin 300142, P.R. China
  • Pages: 406-413
    |
    Published online on: October 5, 2018
       https://doi.org/10.3892/ol.2018.9552
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Abstract

The present study aims to investigate the effect of miR‑30a on the proliferative and invasive abilities of breast cancer cells, and to observe the role of miR‑30a in the pathogenesis of breast cancer. With the increase of pathological grade and malignant degree of breast cancer cells, the miR‑30a expression level gradually decreased (P<0.01). Transfection with miR‑30a mimic significantly inhibited the proliferative and invasive ability of SK‑BR‑3 cells (P<0.01), while transfection with anti‑miR‑30a significantly improved the proliferative and invasive ability of these cells (P<0.01). It was revealed using bioinformatic methods that Snail was the functional target gene of miR‑30a, and this was verified by the results of a luciferase reporter gene assay. The results of analysis of Snail expression in breast cancer tissues and breast cancer cells revealed that with the increase in pathological grade and malignant degree of breast cancer cells, Snail expression levels gradually increased (P<0.01). Western blotting revealed that miR‑30a significantly inhibited Snail expression in SK‑BR‑3 cells, upregulated the expression of EMT‑associated E‑cadherin, and downregulated the expression of EMT‑associated N‑cadherin and Vimentin. MiR‑30a was able to affect the proliferation and invasion of breast cancer cells by regulating Snail expression, and therefore has a regulatory effect on the occurrence and development of breast cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Xiao B, Shi X and Bai J: miR‑30a regulates the proliferation and invasion of breast cancer cells by targeting Snail. Oncol Lett 17: 406-413, 2019.
APA
Xiao, B., Shi, X., & Bai, J. (2019). miR‑30a regulates the proliferation and invasion of breast cancer cells by targeting Snail. Oncology Letters, 17, 406-413. https://doi.org/10.3892/ol.2018.9552
MLA
Xiao, B., Shi, X., Bai, J."miR‑30a regulates the proliferation and invasion of breast cancer cells by targeting Snail". Oncology Letters 17.1 (2019): 406-413.
Chicago
Xiao, B., Shi, X., Bai, J."miR‑30a regulates the proliferation and invasion of breast cancer cells by targeting Snail". Oncology Letters 17, no. 1 (2019): 406-413. https://doi.org/10.3892/ol.2018.9552
Copy and paste a formatted citation
x
Spandidos Publications style
Xiao B, Shi X and Bai J: miR‑30a regulates the proliferation and invasion of breast cancer cells by targeting Snail. Oncol Lett 17: 406-413, 2019.
APA
Xiao, B., Shi, X., & Bai, J. (2019). miR‑30a regulates the proliferation and invasion of breast cancer cells by targeting Snail. Oncology Letters, 17, 406-413. https://doi.org/10.3892/ol.2018.9552
MLA
Xiao, B., Shi, X., Bai, J."miR‑30a regulates the proliferation and invasion of breast cancer cells by targeting Snail". Oncology Letters 17.1 (2019): 406-413.
Chicago
Xiao, B., Shi, X., Bai, J."miR‑30a regulates the proliferation and invasion of breast cancer cells by targeting Snail". Oncology Letters 17, no. 1 (2019): 406-413. https://doi.org/10.3892/ol.2018.9552
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