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Downregulation of LPXN expression by siRNA decreases the malignant proliferation and transmembrane invasion of SHI‑1 cells

  • Authors:
    • Guo‑Hua Zhu
    • Hai‑Ping Dai
    • Qun Shen
    • Qi Zhang
  • View Affiliations / Copyright

    Affiliations: First Clinical College, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, P.R. China, Leukemia Research Unit, Jiangsu Institute of Hematology, First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China
    Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 135-140
    |
    Published online on: October 22, 2018
       https://doi.org/10.3892/ol.2018.9605
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Abstract

The aim of the present study was to investigate the effects of decreasing leupaxin (LPXN) expression on the proliferation and invasion of human acute monocytic leukemia SHI‑1 cells. The transfection efficiency of fluorescein amidite (FAM)‑small interfering RNA (siRNA) was determined using flow cytometry, and the protein expression levels of LPXN, phosphorylated (p)‑c‑Jun N‑terminal kinase (JNK), p‑p38 mitogen‑activated protein kinase (p38 MAPK) and p‑extracellular‑signal‑regulated kinase (ERK) were detected by western blot analysis. Proliferation was determined using the cell counting kit‑8 reagent and cellular transmembrane invasion ability was determined using a Transwell chamber system. The gelatinase levels of matrix metalloproteinase (MMP)‑2 and MMP‑9 in the cell culture supernatant were also analyzed by gelatin zymography. In SHI‑1 cells, the optimal transfection conditions of siRNA were a cell density of 4x105 cells/ml and a ratio of siRNA/Lipofectamine® 2000 of 200 pmol/1 µl. The highest transfection efficiency of FAM‑siRNA was 74.5%. In the present study, L2‑siRNA was selected to effectively decrease the expression of LPXN. Following downregulation of LPXN expression by L2‑siRNA, proliferation inhibition rates increased to 27.043±2.051 and cell transmembrane invasion rates decreased to 25.270±2.145 (P<0.05). The results of the western blot analysis and the gelatin zymography indicated that downregulation of LPXN expression increased the expression of p‑p38 MAPK and p‑JNK, and attenuated the secretion levels of MMP‑2 and MMP‑9. However, downregulation of LPXN expression had no effect on p‑ERK expression in SHI‑1 cells. The results of the present study indicated that downregulation of LPXN expression decreased the malignant proliferation and transmembrane invasion of SHI‑1 cells by activating JNK and p38 MAPK, and inhibiting MMP‑2 and MMP‑9 secretion.
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Copy and paste a formatted citation
Spandidos Publications style
Zhu GH, Dai HP, Shen Q and Zhang Q: Downregulation of LPXN expression by siRNA decreases the malignant proliferation and transmembrane invasion of SHI‑1 cells. Oncol Lett 17: 135-140, 2019.
APA
Zhu, G., Dai, H., Shen, Q., & Zhang, Q. (2019). Downregulation of LPXN expression by siRNA decreases the malignant proliferation and transmembrane invasion of SHI‑1 cells. Oncology Letters, 17, 135-140. https://doi.org/10.3892/ol.2018.9605
MLA
Zhu, G., Dai, H., Shen, Q., Zhang, Q."Downregulation of LPXN expression by siRNA decreases the malignant proliferation and transmembrane invasion of SHI‑1 cells". Oncology Letters 17.1 (2019): 135-140.
Chicago
Zhu, G., Dai, H., Shen, Q., Zhang, Q."Downregulation of LPXN expression by siRNA decreases the malignant proliferation and transmembrane invasion of SHI‑1 cells". Oncology Letters 17, no. 1 (2019): 135-140. https://doi.org/10.3892/ol.2018.9605
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu GH, Dai HP, Shen Q and Zhang Q: Downregulation of LPXN expression by siRNA decreases the malignant proliferation and transmembrane invasion of SHI‑1 cells. Oncol Lett 17: 135-140, 2019.
APA
Zhu, G., Dai, H., Shen, Q., & Zhang, Q. (2019). Downregulation of LPXN expression by siRNA decreases the malignant proliferation and transmembrane invasion of SHI‑1 cells. Oncology Letters, 17, 135-140. https://doi.org/10.3892/ol.2018.9605
MLA
Zhu, G., Dai, H., Shen, Q., Zhang, Q."Downregulation of LPXN expression by siRNA decreases the malignant proliferation and transmembrane invasion of SHI‑1 cells". Oncology Letters 17.1 (2019): 135-140.
Chicago
Zhu, G., Dai, H., Shen, Q., Zhang, Q."Downregulation of LPXN expression by siRNA decreases the malignant proliferation and transmembrane invasion of SHI‑1 cells". Oncology Letters 17, no. 1 (2019): 135-140. https://doi.org/10.3892/ol.2018.9605
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