MIR31HG promotes cell proliferation and invasion by activating the Wnt/β‑catenin signaling pathway in non‑small cell lung cancer

Zheng,Shuaiyu; Zhang,Xiaojin; Wang,Xian; Li,Jiyuan

doi:10.3892/ol.2018.9607

MIR31HG promotes cell proliferation and invasion by activating the Wnt/β‑catenin signaling pathway in non‑small cell lung cancer

Authors:
- Shuaiyu Zheng
- Xiaojin Zhang
- Xian Wang
- Jiyuan Li
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Affiliations: Department of Thoracic Surgery, The First Affiliated Hospital and College of Clinical Medicine of Henan University of Science and Technology, Luoyang, Henan 471003, P.R. China
Published online on: October 23, 2018 https://doi.org/10.3892/ol.2018.9607
Pages: 221-229
Copyright: © Zheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 4.0].

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Abstract

Long non‑coding RNAs (lncRNAs) have recently been demonstrated to serve crucial roles in various diseases including tumor initiation and progression. However, the role of the lncRNA MIR31HG in non‑small cell lung cancer (NSCLC) was not well established. The present study demonstrated that MIR31HG was significantly increased in tumor tissues compared with adjacent normal tissues, and increased MIR31HG expression levels were associated with histological differentiation grade, lymph node metastasis and Tumor‑node metastasis (TNM) stage in patients with NSCLC. Patients who had a higher MIR31HG expression level, were predicted a shorter over survival (OS) time. Using in vitro assays, the present study demonstrated that the downregulation of MIR31HG expression significantly inhibited cell proliferation and cell invasion abilities. Furthermore, it was identified that knockdown of MIR31HG expression suppressed the cell epithelial‑mesenchymal transition (EMT) phenotype by reducing the expression levels of Twist1 and Vimentin, but also increased the expression level of E‑cadherin in NSCLC cells. Furthermore, the results of the present study demonstrated that downregulated MIR31HG inhibited the Wnt/β‑catenin signaling pathway by decreasing the expression of glycogen synthase kinase 3β (GSK3β) and β‑catenin, but increasing the phosphorylated (p)‑GSK3β expression in NSCLC cells. Together, these data demonstrated that MIR31HG could be identified as a poor prognostic biomarker and a novel therapeutic target for patients with NSCLC.

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