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Identification of plasma RGS18 and PPBP mRNAs as potential biomarkers for gastric cancer using transcriptome arrays

  • Authors:
    • Chen Su
    • Hanwei Li
    • Zheng Peng
    • Dong Ke
    • Hanjiang Fu
    • Xiaofei Zheng
  • View Affiliations / Copyright

    Affiliations: Beijing Key Laboratory for Radiobiology, The Beijing Institute of Radiation Medicine, Beijing 100850, P.R. China, Department of General Surgery, The General Hospital of Chinese People's Liberation Army, Beijing 100851, P.R. China, Department of Gastrointestinal Surgery, The Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China
    Copyright: © Su et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 247-255
    |
    Published online on: October 23, 2018
       https://doi.org/10.3892/ol.2018.9608
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Abstract

Coding and noncoding RNAs serve a crucial role in tumorigenesis. Circulating RNAs have been recognized as a novel category of biomarkers for a variety of physiological and pathological conditions. To identify plasma RNA biomarkers for gastric cancer (GC), a genome‑wide transcriptome analysis using GeneChip® Human Transcriptome Array, which contains probe sets covering exons of ~67500 coding and noncoding transcripts of annotated genes, was performed to screen for the RNAs that exhibited differential expression in the plasma samples of patients with GC and controls. The expression levels of 6 candidate RNAs, including regulator of G‑protein signaling 18 (RGS18), integral membrane protein 2B, pro‑platelet basic protein (PPBP), nucleosome assembly protein1‑like 1, n324674 and ENST00000442382 were assessed in the plasma samples of 81 patients with GC and 77 healthy participants using reverse transcription‑quantitative polymerase chain reaction. Furthermore, the expression levels of RGS18 and PPBP mRNAs were indicated to be significantly differentially expressed (P<0.0001) in an independent panel of plasma samples of 36 patients with GC compared with 34 healthy participants. The potential association of RGS18 and PPBP mRNA expression levels with clinicopathological features was subsequently analyzed. Receiver operating characteristic analysis indicated that the combination of these 2 mRNAs with an area under curve <0.812 was an improved indicator for gastric cancer compared with respective individual levels. The results of the present study indicate that RGS18 and PPBP mRNA expression was significantly downregulated in the plasma of patients with GC, and the combination of these 2 mRNAs may be a useful diagnostic or prognostic marker for GC.
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Copy and paste a formatted citation
Spandidos Publications style
Su C, Li H, Peng Z, Ke D, Fu H and Zheng X: Identification of plasma RGS18 and PPBP mRNAs as potential biomarkers for gastric cancer using transcriptome arrays. Oncol Lett 17: 247-255, 2019.
APA
Su, C., Li, H., Peng, Z., Ke, D., Fu, H., & Zheng, X. (2019). Identification of plasma RGS18 and PPBP mRNAs as potential biomarkers for gastric cancer using transcriptome arrays. Oncology Letters, 17, 247-255. https://doi.org/10.3892/ol.2018.9608
MLA
Su, C., Li, H., Peng, Z., Ke, D., Fu, H., Zheng, X."Identification of plasma RGS18 and PPBP mRNAs as potential biomarkers for gastric cancer using transcriptome arrays". Oncology Letters 17.1 (2019): 247-255.
Chicago
Su, C., Li, H., Peng, Z., Ke, D., Fu, H., Zheng, X."Identification of plasma RGS18 and PPBP mRNAs as potential biomarkers for gastric cancer using transcriptome arrays". Oncology Letters 17, no. 1 (2019): 247-255. https://doi.org/10.3892/ol.2018.9608
Copy and paste a formatted citation
x
Spandidos Publications style
Su C, Li H, Peng Z, Ke D, Fu H and Zheng X: Identification of plasma RGS18 and PPBP mRNAs as potential biomarkers for gastric cancer using transcriptome arrays. Oncol Lett 17: 247-255, 2019.
APA
Su, C., Li, H., Peng, Z., Ke, D., Fu, H., & Zheng, X. (2019). Identification of plasma RGS18 and PPBP mRNAs as potential biomarkers for gastric cancer using transcriptome arrays. Oncology Letters, 17, 247-255. https://doi.org/10.3892/ol.2018.9608
MLA
Su, C., Li, H., Peng, Z., Ke, D., Fu, H., Zheng, X."Identification of plasma RGS18 and PPBP mRNAs as potential biomarkers for gastric cancer using transcriptome arrays". Oncology Letters 17.1 (2019): 247-255.
Chicago
Su, C., Li, H., Peng, Z., Ke, D., Fu, H., Zheng, X."Identification of plasma RGS18 and PPBP mRNAs as potential biomarkers for gastric cancer using transcriptome arrays". Oncology Letters 17, no. 1 (2019): 247-255. https://doi.org/10.3892/ol.2018.9608
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