Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
January-2019 Volume 17 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
January-2019 Volume 17 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Correlation between microsatellite instability and RAS gene mutation and stage Ⅲ colorectal cancer

  • Authors:
    • Wenbo Niu
    • Guiying Wang
    • Jun Feng
    • Zheng Li
    • Chenhui Li
    • Baoen Shan
  • View Affiliations / Copyright

    Affiliations: Department of Surgery Ⅱ, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, P.R. China, Center of Scientific Research, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, P.R. China
    Copyright: © Niu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 332-338
    |
    Published online on: October 23, 2018
       https://doi.org/10.3892/ol.2018.9611
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Correlation between RAS gene mutation and microsatellite instabi­lity (MSI) status in cancer tissues and clinicopathological parameters of patients with stage Ⅲ colorectal cancer (CRC) were investigated. Tissues were collected from 180 patients diagnosed with stage Ⅲ CRC in the Department of Gastrointestinal Surgery of the Fourth Hospital of Hebei Medical University from 2012 to 2016. RAS gene mutations in paraffin sections were detected by PCR and Sanger sequencing. Expression of mismatch repair proteins MLH1, MSH2, MSH6 and PMS2 was detected by immunohistochemistry, and MSI status was determined based on the positive and negative expression combinations of the above proteins, and the correlation with clinicopathological parameters of CRC was analyzed. Mutation rates of KRAS and NRAS were 48.33% (87/180) and 2.78% (5/180), respectively. Mutation rate of p.G12D in codon 12 of exon 2 in KRAS gene was the highest (31/87, 35.63%). Mutation rate of p.G12D in codon 12 of exon 2 in NRAS gene was the highest (2/5, 40%). Mutation rate of KRAS gene in right colon was higher than that in left colon and rectum (p<0.05), and mutation rate in N2b phase was higher than that in N2a and N1 phases (p<0.01). In low degree of microsatellite instability (MSI‑L) and high degree of microsatellite instability (MSI‑H) status, negative MKH1 protein expression was dominant (18/32, 56.25%). MSI‑H in CRC patients aged ≥50 years was higher than that of CRC patients <50 years. Rates of MSI‑H in N1, N2a, and N2b were 1.75, 12.82, and 1.11% (p<0.05). Mutation rate of KRAS gene in MSI‑H status of stage Ⅲ CRC patients was significantly higher than that in MSI‑L/microsatellite stability (MSS) (p<0.05). Mutation of RAS gene and the status of MSI are involved in the occurrence and development of stage Ⅲ CRC. Detection of RAS gene has important significance for the individual treatment of CRC in clinic.
View Figures

Figure 1

View References

1 

Martins C, Sousa P, Araújo T, Castro-Poças F and Pedroto I: Mediastinal mass in a patient with colorectal cancer: A diagnostic challenge. GE Port J Gastroenterol. 24:193–197. 2017. View Article : Google Scholar : PubMed/NCBI

2 

Cermak K, Thill V, Simoens CH, Smets D, Ngongang CH and da Costa PM: Surgical resection for colon cancer: laparoscopic assisted vs. open colectomy. Hepatogastroenterology. 55:412–417. 2008.PubMed/NCBI

3 

Heo JH, Ryu CG, Jung EJ, Paik JH and Hwang DY: Clinical significance of preoperative virtual colonoscopy for evaluation of the proximal colon in patient with obstructive colorectal cancer. Ann Coloproctol. 33:130–133. 2017. View Article : Google Scholar : PubMed/NCBI

4 

Wicha MS: Targeting self-renewal, an Achilles' heel of cancer stem cells. Nat Med. 20:14–15. 2014. View Article : Google Scholar : PubMed/NCBI

5 

Jiricny J: The multifaceted mismatch-repair system. Nat Rev Mol Cell Biol. 7:335–346. 2006. View Article : Google Scholar : PubMed/NCBI

6 

Iyer RR, Pluciennik A, Burdett V and Modrich PL: DNA mismatch repair: Functions and mechanisms. Chem Rev. 106:302–323. 2006. View Article : Google Scholar : PubMed/NCBI

7 

Barberán S, Fraguas S and Cebrià F: The EGFR signaling pathway controls gut progenitor differentiation during planarian regeneration and homeostasis. Development. 143:2089–2102. 2016. View Article : Google Scholar : PubMed/NCBI

8 

Saafan H, Foerster S, Parra-Guillen ZP, Hammer E, Michaelis M, Cinatl J Jr, Völker U, Fröhlich H, Kloft C and Ritter CA: Utilising the EGFR interactome to identify mechanisms of drug resistance in non-small cell lung cancer - proof of concept towards a systems pharmacology approach. Eur J Pharm Sci. 94:20–32. 2016. View Article : Google Scholar : PubMed/NCBI

9 

Tang Y, Zhou H, Chen A, Pittman RN and Field J: The Akt proto-oncogene links Ras to Pak and cell survival signals. J Biol Chem. 275:9106–9109. 2000. View Article : Google Scholar : PubMed/NCBI

10 

Di Nicolantonio F, Martini M, Molinari F, Sartore-Bianchi A, Arena S, Saletti P, De Dosso S, Mazzucchelli L, Frattini M, Siena S, et al: Wild-type BRAF is required for response to panitumumab or cetuximab in metastatic colorectal cancer. J Clin Oncol. 26:5705–5712. 2008. View Article : Google Scholar : PubMed/NCBI

11 

Trojan J, Mineur L, Tomášek J, Rouleau E, Fabian P, de Maglio G, García-Alfonso P, Aprile G, Taylor A, Kafatos G, et al: Panitumumab use in metastatic colorectal cancer and patterns of KRAS testing: Results from a Europe-wide physician survey and medical records review. PLoS One. 10:e01407172015. View Article : Google Scholar : PubMed/NCBI

12 

Hu J, Yan WY, Xie L, Cheng L, Yang M, Li L, Shi J, Liu BR and Qian XP: Coexistence of MSI with KRAS mutation is associated with worse prognosis in colorectal cancer. Medicine (Baltimore). 95:e56492016. View Article : Google Scholar : PubMed/NCBI

13 

Sideris M and Papagrigoriadis S: Molecular biomarkers and classification models in the evaluation of the prognosis of colorectal cancer. Anticancer Res. 34:2061–2068. 2014.PubMed/NCBI

14 

Slik K, Kurki S, Korpela T, Carpén O, Korkeila E and Sundström J: Ezrin expression combined with MSI status in prognostication of stage II colorectal cancer. PLoS One. 12:e01854362017. View Article : Google Scholar : PubMed/NCBI

15 

Ciardiello F and Tortora G: EGFR antagonists in cancer treatment. N Engl J Med. 358:1160–1174. 2008. View Article : Google Scholar : PubMed/NCBI

16 

Neumann J, Zeindl-Eberhart E, Kirchner T and Jung A: Frequency and type of KRAS mutations in routine diagnostic analysis of metastatic colorectal cancer. Pathol Res Pract. 205:858–862. 2009. View Article : Google Scholar : PubMed/NCBI

17 

Shemirani AI, Haghighi MM, Milanizadeh S, Taleghani MY, Fatemi SR, Damavand B, Akbari Z and Zali MR: The role of kras mutations and MSI status in diagnosis of colorectal cancer. Gastroenterol Hepatol Bed Bench. 4:70–75. 2011.PubMed/NCBI

18 

Vilar E and Gruber SB: Microsatellite instability in colorectal cancer-the stable evidence. Nat Rev Clin Oncol. 7:153–162. 2010. View Article : Google Scholar : PubMed/NCBI

19 

Philip P, Ernst P and Wantzin GL: Karyotypes in infectious mononucleosis. Scand J Haematol. 15:201–206. 1975. View Article : Google Scholar : PubMed/NCBI

20 

Kambara T, Simms LA, Whitehall VL, Spring KJ, Wynter CV, Walsh MD, Barker MA, Arnold S, McGivern A, Matsubara N, et al: BRAF mutation is associated with DNA methylation in serrated polyps and cancers of the colorectum. Gut. 53:1137–1144. 2004. View Article : Google Scholar : PubMed/NCBI

21 

Ye JX, Liu Y, Qin Y, Zhong HH, Yi WN and Shi XY: KRAS and BRAF gene mutations and DNA mismatch repair status in Chinese colorectal carcinoma patients. World J Gastroenterol. 21:1595–1605. 2015. View Article : Google Scholar : PubMed/NCBI

22 

Omidifar N, Geramizadeh B and Mirzai M: K-ras mutation in colorectal cancer, a report from Southern Iran. Iran J Med Sci. 40:454–460. 2015.PubMed/NCBI

23 

Fujiyoshi K, Yamamoto G, Takahashi A, Arai Y, Yamada M, Kakuta M, Yamaguchi K, Akagi Y, Nishimura Y, Sakamoto H, et al: High concordance rate of KRAS/BRAF mutations and MSI-H between primary colorectal cancer and corresponding metastases. Oncol Rep. 37:785–792. 2017. View Article : Google Scholar : PubMed/NCBI

24 

Martinetti D, Costanzo R, Kadare S, Alimehmeti M, Colarossi C, Canzonieri V, Berretta M and Memeo L: KRAS and BRAF mutational status in colon cancer from Albanian patients. Diagn Pathol. 9:1872014. View Article : Google Scholar : PubMed/NCBI

25 

Kawazoe A, Shitara K, Fukuoka S, Kuboki Y, Bando H, Okamoto W, Kojima T, Fuse N, Yamanaka T, Doi T, et al: A retrospective observational study of clinicopathological features of KRAS NRAS, BRAF and PIK3CA mutations in Japanese patients with metastatic colorectal cancer. BMC Cancer. 15:2582015. View Article : Google Scholar : PubMed/NCBI

26 

Ogura T, Kakuta M, Yatsuoka T, Nishimura Y, Sakamoto H, Yamaguchi K, Tanabe M, Tanaka Y and Akagi K: Clinicopathological characteristics and prognostic impact of colorectal cancers with NRAS mutations. Oncol Rep. 32:50–56. 2014. View Article : Google Scholar : PubMed/NCBI

27 

Yamane LS, Scapulatempo-Neto C, Alvarenga L, Oliveira CZ, Berardinelli GN, Almodova E, Cunha TR, Fava G, Colaiacovo W, Melani A, et al: KRAS and BRAF mutations and MSI status in precursor lesions of colorectal cancer detected by colonoscopy. Oncol Rep. 32:1419–1426. 2014. View Article : Google Scholar : PubMed/NCBI

28 

Fleming M, Ravula S, Tatishchev SF and Wang HL: Colorectal carcinoma: Pathologic aspects. J Gastrointest Oncol. 3:153–173. 2012.PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Niu W, Wang G, Feng J, Li Z, Li C and Shan B: Correlation between microsatellite instability and RAS gene mutation and stage Ⅲ colorectal cancer. Oncol Lett 17: 332-338, 2019.
APA
Niu, W., Wang, G., Feng, J., Li, Z., Li, C., & Shan, B. (2019). Correlation between microsatellite instability and RAS gene mutation and stage Ⅲ colorectal cancer. Oncology Letters, 17, 332-338. https://doi.org/10.3892/ol.2018.9611
MLA
Niu, W., Wang, G., Feng, J., Li, Z., Li, C., Shan, B."Correlation between microsatellite instability and RAS gene mutation and stage Ⅲ colorectal cancer". Oncology Letters 17.1 (2019): 332-338.
Chicago
Niu, W., Wang, G., Feng, J., Li, Z., Li, C., Shan, B."Correlation between microsatellite instability and RAS gene mutation and stage Ⅲ colorectal cancer". Oncology Letters 17, no. 1 (2019): 332-338. https://doi.org/10.3892/ol.2018.9611
Copy and paste a formatted citation
x
Spandidos Publications style
Niu W, Wang G, Feng J, Li Z, Li C and Shan B: Correlation between microsatellite instability and RAS gene mutation and stage Ⅲ colorectal cancer. Oncol Lett 17: 332-338, 2019.
APA
Niu, W., Wang, G., Feng, J., Li, Z., Li, C., & Shan, B. (2019). Correlation between microsatellite instability and RAS gene mutation and stage Ⅲ colorectal cancer. Oncology Letters, 17, 332-338. https://doi.org/10.3892/ol.2018.9611
MLA
Niu, W., Wang, G., Feng, J., Li, Z., Li, C., Shan, B."Correlation between microsatellite instability and RAS gene mutation and stage Ⅲ colorectal cancer". Oncology Letters 17.1 (2019): 332-338.
Chicago
Niu, W., Wang, G., Feng, J., Li, Z., Li, C., Shan, B."Correlation between microsatellite instability and RAS gene mutation and stage Ⅲ colorectal cancer". Oncology Letters 17, no. 1 (2019): 332-338. https://doi.org/10.3892/ol.2018.9611
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team