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Article Open Access

Gadofosveset‑enhanced MRI as simple surrogate parameter for real‑time evaluation of the initial tumour vessel infarction by retargeted tissue factor tTF‑NGR

  • Authors:
    • Anna Höink
    • Thorsten Persigehl
    • Robert Kwiecien
    • Martin Balthasar
    • Rolf Mesters
    • Wolfgang Berdel
    • Walter Heindel
    • Christoph Bremer
    • Christian Schwöppe
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Radiology, University Hospital Münster, D‑48149 Münster, Germany, Institute of Biostatistics and Clinical Research, University of Münster, D‑48149 Münster, Germany, Department of Diagnostic and Interventional Radiology, University Hospital Cologne, D‑50937 Cologne, Germany, Department of Medicine A ‑ Haematology and Oncology, University Hospital Münster, D‑48149 Münster, Germany, Department of Radiology, St. Franziskus‑Hospital Münster, D‑48145 Münster, Germany
    Copyright: © Höink et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 270-280
    |
    Published online on: October 29, 2018
       https://doi.org/10.3892/ol.2018.9638
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Abstract

Truncated tissue factor (tTF)‑NGR consists of the extracellular domain of the human TF and the binding motif NGR. tTF‑NGR activates blood coagulation within the tumour vasculature following binding to CD13, and is overexpressed in the endothelial cells of tumour vessels, resulting in tumour vessel infarction and subsequent retardation/regression of tumour growth. The aim of the present study was to investigate gadofosveset‑based real‑time dynamic contrast-enhanced magnetic resonance imaging (DCE‑MRI) in evaluating the initial therapeutic effects of the anti‑vascular tTF‑NGR approach. DCE‑MRI (3.0 T) was performed in human U87‑glioblastoma tumour‑bearing nude mice. During a dynamic T1w GE‑sequence, a gadolinium‑based blood pool contrast agent (gadofosveset) was injected via a tail vein catheter. Following the maximum contrast intensity inside the tumour being obtained, tTF‑NGR was injected (controls received NaCl) and the contrast behaviour of the tumour was monitored by ROI analysis. The slope difference of signal intensities between controls and the tTF‑NGR group was investigated, as well as the differences between the average area under the curve (AUC) of the two groups. The association between intensity, group (control vs. tTF‑NGR group) and time was analysed by fitting a linear mixed model. Following the injection of tTF‑NGR, the signal intensity inside the tumours exhibited a statistically significantly stronger average slope decrease compared with the signal intensity of the tumours in the NaCl group. Furthermore, the initial average AUC values of mice treated with tTF‑NGR were 5.7% lower than the average AUC of the control animals (P<0.05). Gadofosveset‑enhanced MRI enables the visualization of the initial tumour response to anti‑vascular treatment in real‑time. Considering the clinical application of tTF‑NGR, this method may provide a simple alternative parameter for monitoring the tumour response to vascular disrupting agents and certain vascular targeting agents in humans.
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Copy and paste a formatted citation
Spandidos Publications style
Höink A, Persigehl T, Kwiecien R, Balthasar M, Mesters R, Berdel W, Heindel W, Bremer C and Schwöppe C: Gadofosveset‑enhanced MRI as simple surrogate parameter for real‑time evaluation of the initial tumour vessel infarction by retargeted tissue factor tTF‑NGR. Oncol Lett 17: 270-280, 2019.
APA
Höink, A., Persigehl, T., Kwiecien, R., Balthasar, M., Mesters, R., Berdel, W. ... Schwöppe, C. (2019). Gadofosveset‑enhanced MRI as simple surrogate parameter for real‑time evaluation of the initial tumour vessel infarction by retargeted tissue factor tTF‑NGR. Oncology Letters, 17, 270-280. https://doi.org/10.3892/ol.2018.9638
MLA
Höink, A., Persigehl, T., Kwiecien, R., Balthasar, M., Mesters, R., Berdel, W., Heindel, W., Bremer, C., Schwöppe, C."Gadofosveset‑enhanced MRI as simple surrogate parameter for real‑time evaluation of the initial tumour vessel infarction by retargeted tissue factor tTF‑NGR". Oncology Letters 17.1 (2019): 270-280.
Chicago
Höink, A., Persigehl, T., Kwiecien, R., Balthasar, M., Mesters, R., Berdel, W., Heindel, W., Bremer, C., Schwöppe, C."Gadofosveset‑enhanced MRI as simple surrogate parameter for real‑time evaluation of the initial tumour vessel infarction by retargeted tissue factor tTF‑NGR". Oncology Letters 17, no. 1 (2019): 270-280. https://doi.org/10.3892/ol.2018.9638
Copy and paste a formatted citation
x
Spandidos Publications style
Höink A, Persigehl T, Kwiecien R, Balthasar M, Mesters R, Berdel W, Heindel W, Bremer C and Schwöppe C: Gadofosveset‑enhanced MRI as simple surrogate parameter for real‑time evaluation of the initial tumour vessel infarction by retargeted tissue factor tTF‑NGR. Oncol Lett 17: 270-280, 2019.
APA
Höink, A., Persigehl, T., Kwiecien, R., Balthasar, M., Mesters, R., Berdel, W. ... Schwöppe, C. (2019). Gadofosveset‑enhanced MRI as simple surrogate parameter for real‑time evaluation of the initial tumour vessel infarction by retargeted tissue factor tTF‑NGR. Oncology Letters, 17, 270-280. https://doi.org/10.3892/ol.2018.9638
MLA
Höink, A., Persigehl, T., Kwiecien, R., Balthasar, M., Mesters, R., Berdel, W., Heindel, W., Bremer, C., Schwöppe, C."Gadofosveset‑enhanced MRI as simple surrogate parameter for real‑time evaluation of the initial tumour vessel infarction by retargeted tissue factor tTF‑NGR". Oncology Letters 17.1 (2019): 270-280.
Chicago
Höink, A., Persigehl, T., Kwiecien, R., Balthasar, M., Mesters, R., Berdel, W., Heindel, W., Bremer, C., Schwöppe, C."Gadofosveset‑enhanced MRI as simple surrogate parameter for real‑time evaluation of the initial tumour vessel infarction by retargeted tissue factor tTF‑NGR". Oncology Letters 17, no. 1 (2019): 270-280. https://doi.org/10.3892/ol.2018.9638
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