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Extracellular matrix differences in glioblastoma patients with different prognoses

  • Authors:
    • József Virga
    • László Szivos
    • Tibor Hortobágyi
    • Mahan Kouhsari Chalsaraei
    • Gábor Zahuczky
    • László Steiner
    • Judit Tóth
    • Judit Reményi‑Puskár
    • László Bognár
    • Almos Klekner
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, Faculty of Medicine, University of Debrecen, H-4032 Debrecen, Hungary, MTA‑DE Cerebrovascular and Neurodegenerative Research Group, Department of Neuropathology, Institute of Pathology, University of Debrecen, H-4032 Debrecen, Hungary, UD Genomed Ltd., H-4032 Debrecen, Hungary, Department of Oncology, Faculty of Medicine, University of Debrecen, H-4032 Debrecen, Hungary
    Copyright: © Virga et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY 4.0].
  • Pages: 797-806
    |
    Published online on: October 31, 2018
       https://doi.org/10.3892/ol.2018.9649
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Abstract

Glioblastoma is the most common malignant central nervous system tumor. Patient outcome remains poor despite the development of therapy and increased understanding of the disease in the past decades. Glioma cells invade the peritumoral brain, which results in inevitable tumor recurrence. Previous studies have demonstrated that the extracellular matrix (ECM) is altered in gliomas and serves a major role in glioma invasion. The present study focuses on differences in the ECM composition of tumors in patients with poor and improved prognosis. The mRNA and protein expression of 16 invasion‑associated ECM molecules was determined using reverse trascription‑quantitiative polymerase chain reaction and immunohistochemistry, respectively. Clinical factors of patients with different prognoses was also analyzed. It was determined that age and postoperative Karnofsky performance score were associated with patient survival. Furthermore, Fms‑related tyrosine kinase 4/vascular endothelial growth factor receptor 3 (FLT4/VEGFR3), murine double minute 2 (MDM2) and matrix metallopeptidase 2 (MMP2) mRNA levels were significantly different between the two prognostic groups. Additionally, brevican, cluster of differentiation 44, hyaluronan mediated motility receptor, integrin‑αV and ‑β1, and MDM2 protein expression were indicated to be significantly different in immunohistochemistry slides. Using the expression profile, including the invasion spectrum of the samples, it was possible to identify the prognostic group of the sample with high efficacy, particularly in cases with poor prognosis. In conclusion, it was determined that ECM components exhibit different expression levels in tumors with different prognoses and thus the invasion spectrum can be used as a prognostic factor in glioblastoma.
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Spandidos Publications style
Virga J, Szivos L, Hortobágyi T, Chalsaraei MK, Zahuczky G, Steiner L, Tóth J, Reményi‑Puskár J, Bognár L, Klekner A, Klekner A, et al: Extracellular matrix differences in glioblastoma patients with different prognoses. Oncol Lett 17: 797-806, 2019.
APA
Virga, J., Szivos, L., Hortobágyi, T., Chalsaraei, M.K., Zahuczky, G., Steiner, L. ... Klekner, A. (2019). Extracellular matrix differences in glioblastoma patients with different prognoses. Oncology Letters, 17, 797-806. https://doi.org/10.3892/ol.2018.9649
MLA
Virga, J., Szivos, L., Hortobágyi, T., Chalsaraei, M. K., Zahuczky, G., Steiner, L., Tóth, J., Reményi‑Puskár, J., Bognár, L., Klekner, A."Extracellular matrix differences in glioblastoma patients with different prognoses". Oncology Letters 17.1 (2019): 797-806.
Chicago
Virga, J., Szivos, L., Hortobágyi, T., Chalsaraei, M. K., Zahuczky, G., Steiner, L., Tóth, J., Reményi‑Puskár, J., Bognár, L., Klekner, A."Extracellular matrix differences in glioblastoma patients with different prognoses". Oncology Letters 17, no. 1 (2019): 797-806. https://doi.org/10.3892/ol.2018.9649
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Spandidos Publications style
Virga J, Szivos L, Hortobágyi T, Chalsaraei MK, Zahuczky G, Steiner L, Tóth J, Reményi‑Puskár J, Bognár L, Klekner A, Klekner A, et al: Extracellular matrix differences in glioblastoma patients with different prognoses. Oncol Lett 17: 797-806, 2019.
APA
Virga, J., Szivos, L., Hortobágyi, T., Chalsaraei, M.K., Zahuczky, G., Steiner, L. ... Klekner, A. (2019). Extracellular matrix differences in glioblastoma patients with different prognoses. Oncology Letters, 17, 797-806. https://doi.org/10.3892/ol.2018.9649
MLA
Virga, J., Szivos, L., Hortobágyi, T., Chalsaraei, M. K., Zahuczky, G., Steiner, L., Tóth, J., Reményi‑Puskár, J., Bognár, L., Klekner, A."Extracellular matrix differences in glioblastoma patients with different prognoses". Oncology Letters 17.1 (2019): 797-806.
Chicago
Virga, J., Szivos, L., Hortobágyi, T., Chalsaraei, M. K., Zahuczky, G., Steiner, L., Tóth, J., Reményi‑Puskár, J., Bognár, L., Klekner, A."Extracellular matrix differences in glioblastoma patients with different prognoses". Oncology Letters 17, no. 1 (2019): 797-806. https://doi.org/10.3892/ol.2018.9649
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