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Article Open Access

Effect of HMGN2 on proliferation and apoptosis of MCF-7 breast cancer cells

Retraction in: /10.3892/ol.2025.15005
  • Authors:
    • Bo Fan
    • Sifeng Shi
    • Xiaofei Shen
    • Xiaolong Yang
    • Na Liu
    • Guixia Wu
    • Xiaojuan Guo
    • Ning Huang
  • View Affiliations / Copyright

    Affiliations: Department of Pathophysiology, Research Unit of Infection and Immunity, West China College of Basic and Forensic Medicine, Sichuan University, Chengdu, Sichuan 610041, P.R. China, Department of Orthopedics, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221000, P.R. China, Department of Physiology, Xinjiang Medical University, Urumqi, Xinjiang Uygur Autonomous Region 830011, P.R. China
    Copyright: © Fan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1160-1166
    |
    Published online on: November 5, 2018
       https://doi.org/10.3892/ol.2018.9668
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Abstract

We investigated the effect of high mobility group protein N2 (HMGN2) on the proliferation and apoptosis of the human MCF-7 breast cancer cell line, and its effect on tumor growth in a subcutaneous heterotopic transplantation tumor model of breast cancer. The cell viability assay was used to verify the effect of the recombinant human HMGN2 on MCF-7 cell proliferation. The Transwell chamber assay was used to verify the effect of HMGN2 on MCF-7 cell migration. Flow cytometry and Hoechst staining were used to detect the effect of HMGN2 on MCF-7 cell apoptosis. MCF-7 was injected to establish a subcutaneous heterotopic transplantation tumor model of breast cancer in nude mice. The size, weight and volume of tumor in each group were compared after the administration of different concentrations of HMGN2 solution around the tumor tissue at day 1, 3, 5 and 7. The tumor tissue was removed and cut into sections, and the apoptotic cells in tumors of nude mice were detected by a TUNEL kit. The CCK-8 assay showed that HMGN2 at different concentrations inhibited the proliferation of the MCF-7 breast cancer cells, and the proliferation of MCF-7 cells were significantly inhibited when the concentration of HMGN2 reached 3 µg/ml (P<0.01). The Transwell chamber assay showed that 3 µg/ml of HMGN2 significantly decreased the migration capacity of MCF-7 cells (P<0.01). Flow cytometry and Hoechst staining showed that 3 µg/ml of HMGN2 significantly increased apoptosis of MCF-7 cells (P<0.01). After the nude mouse model of breast cancer was established, HMGN2 at different concentrations was injected around the tumor tissue at day 1, 3, 5 and 7. We demonstrated that the growth of breast cancer was significantly inhibited when the concentration of HMGN2 reached 15 µg/ml. TUNEL staining showed that the number of apoptotic cells in the 15 µg/ml dose group was significantly higher than that in the control group (P<0.01). Therefore, in vitro and in vivo experiments proved that recombinant human HMGN2 could significantly inhibit the proliferation and migration of breast cancer cells, which increased the apoptosis of breast cancer cells and exerted anti-breast cancer effects, which enriched our understanding of the biological roles of HMGN2.
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Copy and paste a formatted citation
Spandidos Publications style
Fan B, Shi S, Shen X, Yang X, Liu N, Wu G, Guo X and Huang N: Effect of HMGN2 on proliferation and apoptosis of MCF-7 breast cancer cells Retraction in /10.3892/ol.2025.15005. Oncol Lett 17: 1160-1166, 2019.
APA
Fan, B., Shi, S., Shen, X., Yang, X., Liu, N., Wu, G. ... Huang, N. (2019). Effect of HMGN2 on proliferation and apoptosis of MCF-7 breast cancer cells Retraction in /10.3892/ol.2025.15005. Oncology Letters, 17, 1160-1166. https://doi.org/10.3892/ol.2018.9668
MLA
Fan, B., Shi, S., Shen, X., Yang, X., Liu, N., Wu, G., Guo, X., Huang, N."Effect of HMGN2 on proliferation and apoptosis of MCF-7 breast cancer cells Retraction in /10.3892/ol.2025.15005". Oncology Letters 17.1 (2019): 1160-1166.
Chicago
Fan, B., Shi, S., Shen, X., Yang, X., Liu, N., Wu, G., Guo, X., Huang, N."Effect of HMGN2 on proliferation and apoptosis of MCF-7 breast cancer cells Retraction in /10.3892/ol.2025.15005". Oncology Letters 17, no. 1 (2019): 1160-1166. https://doi.org/10.3892/ol.2018.9668
Copy and paste a formatted citation
x
Spandidos Publications style
Fan B, Shi S, Shen X, Yang X, Liu N, Wu G, Guo X and Huang N: Effect of HMGN2 on proliferation and apoptosis of MCF-7 breast cancer cells Retraction in /10.3892/ol.2025.15005. Oncol Lett 17: 1160-1166, 2019.
APA
Fan, B., Shi, S., Shen, X., Yang, X., Liu, N., Wu, G. ... Huang, N. (2019). Effect of HMGN2 on proliferation and apoptosis of MCF-7 breast cancer cells Retraction in /10.3892/ol.2025.15005. Oncology Letters, 17, 1160-1166. https://doi.org/10.3892/ol.2018.9668
MLA
Fan, B., Shi, S., Shen, X., Yang, X., Liu, N., Wu, G., Guo, X., Huang, N."Effect of HMGN2 on proliferation and apoptosis of MCF-7 breast cancer cells Retraction in /10.3892/ol.2025.15005". Oncology Letters 17.1 (2019): 1160-1166.
Chicago
Fan, B., Shi, S., Shen, X., Yang, X., Liu, N., Wu, G., Guo, X., Huang, N."Effect of HMGN2 on proliferation and apoptosis of MCF-7 breast cancer cells Retraction in /10.3892/ol.2025.15005". Oncology Letters 17, no. 1 (2019): 1160-1166. https://doi.org/10.3892/ol.2018.9668
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