Open Access

Effects of miR-101, miR-345 on HBV replication regulation and on the growth of liver cancer cells

  • Authors:
    • Aikun Ding
    • Cuiyun Wang
    • Lihua Zhang
  • View Affiliations

  • Published online on: November 5, 2018     https://doi.org/10.3892/ol.2018.9669
  • Pages: 1167-1171
  • Copyright: © Ding et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the study was to investigate the effects of miRNA-101 and miRNA-345 on HBV replication and liver cancer cell growth. qPCR was performed to detect the expression of miRNA-101 and miRNA-345. The expression of HBV RNA was detected by PCR. The expression of HbsAg was detected using ELISA. BEL-7404 cell line proliferation was detected by MTT assay. The expression levels of miR-101 and miR-345 in BEL-7404 pSUPER.neo-miR-101 group and BEL-7404 pSUPER.neo-miR-345 group were significantly higher than those in BEL-7404 pSUPER.neo group (P<0.05). The expression levels of miR-101 and miR-345 in MHCC97-L pSUPER.neo-miR-101 group and MHCC97-L pSUPER.neo-miR-345 group were significantly higher than those in MHCC97-L pSUPER.neo group (P<0.05). The expression of HBV DNA in MHCC97-L pSUPER.neo-miR-101 group was significantly lower than that in MHCC97-L pSUPER.neo group (P<0.05), and the expression of HBV DNA in MHCC97-L pSUPER.neo-miR-345 group was significantly higher than that in MHCC97-L pSUPER.neo group (P<0.05). The expression of HbsAg in MHCC97-L pSUPER.neo-miR‑101 group was significantly lower than that in MHCC97-L pSUPER.neo group (P<0.05), and the expression of HbsAg in MHCC97-L pSUPER.neo-miR-345 group was significantly higher than that in MHCC97-L pSUPER.neo group (P<0.05). There was a significant difference in terms of HbsAg expression between the MHCC97-L pSUPER.neo-miR-101 and MHCC97-L pSUPER.neo-miR-345 groups (P<0.05). The proliferation of BEL-7404 cells in the BEL-7404 pSUPER.neo-miR-101 group was significantly lower than that in the BEL-7404 pSUPER.neo group (P<0.05). The proliferation of BEL-7404 cells in the BEL-7404 pSUPER.neo-miR-345 group was significantly higher than that in the BEL-7404 pSUPER.neo group (P<0.05). The proliferation of BEL-7404 cells in BEL-7404 pSUPER.neo-miR-101 group was different from that in BEL-7404 pSUPER.neo-miR-345 group (P<0.05). miR-101 reduced the level of HBV replication, and inhibited the proliferation of liver cancer cells. miR-345 also upregulated the level of HBV replication, and promoted the proliferation of liver cancer cells.
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January-2019
Volume 17 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Ding A, Wang C and Zhang L: Effects of miR-101, miR-345 on HBV replication regulation and on the growth of liver cancer cells. Oncol Lett 17: 1167-1171, 2019
APA
Ding, A., Wang, C., & Zhang, L. (2019). Effects of miR-101, miR-345 on HBV replication regulation and on the growth of liver cancer cells. Oncology Letters, 17, 1167-1171. https://doi.org/10.3892/ol.2018.9669
MLA
Ding, A., Wang, C., Zhang, L."Effects of miR-101, miR-345 on HBV replication regulation and on the growth of liver cancer cells". Oncology Letters 17.1 (2019): 1167-1171.
Chicago
Ding, A., Wang, C., Zhang, L."Effects of miR-101, miR-345 on HBV replication regulation and on the growth of liver cancer cells". Oncology Letters 17, no. 1 (2019): 1167-1171. https://doi.org/10.3892/ol.2018.9669